Lipid Polymorphism of the Subchloroplast-Granum and Stroma
Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy

J 2021

Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy

DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY et. al.

Základní údaje

Originální název

Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy

Autoři

DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY, V. SPUNDA, J. PLAVEC a G. GARAB

Vydání

Blood Cells, Molecules and Diseases, Orlando, Florida, Academic Press, 2021, 1079-9796

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.372

Kód RIV

RIV/00216224:14740/21:00124445

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/cells10092354

UT WoS

000699437700001

Klíčová slova anglicky

P-31-NMR; bilayer membrane; DEM-dynamic exchange model; grana; H-II phase; isotropic phase; non-bilayer lipids; non-lamellar lipid phases; thylakoid membranes; structural flexibility

Štítky

CF CRYO, ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 5. 2022 13:57, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been documented that fully functional thylakoid membranes, in addition to the bilayer, contain an inverted hexagonal (H-II) phase and two isotropic phases. To shed light on the origin of these non-lamellar phases, we performed P-31-NMR spectroscopy experiments on sub-chloroplast particles of spinach: stacked, granum and unstacked, stroma thylakoid membranes. These membranes exhibited similar lipid polymorphism as the whole thylakoids. Saturation transfer experiments, applying saturating pulses at characteristic frequencies at 5 degrees C, provided evidence for distinct lipid phases-with component spectra very similar to those derived from mathematical deconvolution of the P-31-NMR spectra. Wheat-germ lipase treatment of samples selectively eliminated the phases exhibiting sharp isotropic peaks, suggesting easier accessibility of these lipids compared to the bilayer and the H-II phases. Gradually increasing lipid exchanges were observed between the bilayer and the two isotropic phases upon gradually elevating the temperature from 5 to 35 degrees C, suggesting close connections between these lipid phases. Data concerning the identity and structural and functional roles of different lipid phases will be presented in the accompanying paper.

Návaznosti

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY, V. SPUNDA, J. PLAVEC a G. GARAB. Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy. Blood Cells, Molecules and Diseases. Orlando, Florida: Academic Press, 2021, roč. 10, č. 9, s. 2354-2369. ISSN 1079-9796. Dostupné z: https://dx.doi.org/10.3390/cells10092354.

@article{1842616,
   author = {Dlouhy, O. and Javornik, U. and Zsiros, O. and Sket, P. and Karlicky, V. and Spunda, V. and Plavec, J. and Garab, G.},
   article_location = {Orlando, Florida},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/cells10092354},
   keywords = {P-31-NMR; bilayer membrane; DEM-dynamic exchange model; grana; H-II phase; isotropic phase; non-bilayer lipids; non-lamellar lipid phases; thylakoid membranes; structural flexibility},
   language = {eng},
   issn = {1079-9796},
   journal = {Blood Cells, Molecules and Diseases},
   title = {Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy},
   url = {https://www.mdpi.com/2073-4409/10/9/2354},
   volume = {10},
   year = {2021}
}

TY  - JOUR
ID  - 1842616
AU  - Dlouhy, O. - Javornik, U. - Zsiros, O. - Sket, P. - Karlicky, V. - Spunda, V. - Plavec, J. - Garab, G.
PY  - 2021
TI  - Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy
JF  - Blood Cells, Molecules and Diseases
VL  - 10
IS  - 9
SP  - 2354
EP  - 2354
PB  - Academic Press
SN  - 10799796
KW  - P-31-NMR
KW  - bilayer membrane
KW  - DEM-dynamic exchange model
KW  - grana
KW  - H-II phase
KW  - isotropic phase
KW  - non-bilayer lipids
KW  - non-lamellar lipid phases
KW  - thylakoid membranes
KW  - structural flexibility
UR  - https://www.mdpi.com/2073-4409/10/9/2354
N2  - Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been documented that fully functional thylakoid membranes, in addition to the bilayer, contain an inverted hexagonal (H-II) phase and two isotropic phases. To shed light on the origin of these non-lamellar phases, we performed P-31-NMR spectroscopy experiments on sub-chloroplast particles of spinach: stacked, granum and unstacked, stroma thylakoid membranes. These membranes exhibited similar lipid polymorphism as the whole thylakoids. Saturation transfer experiments, applying saturating pulses at characteristic frequencies at 5 degrees C, provided evidence for distinct lipid phases-with component spectra very similar to those derived from mathematical deconvolution of the P-31-NMR spectra. Wheat-germ lipase treatment of samples selectively eliminated the phases exhibiting sharp isotropic peaks, suggesting easier accessibility of these lipids compared to the bilayer and the H-II phases. Gradually increasing lipid exchanges were observed between the bilayer and the two isotropic phases upon gradually elevating the temperature from 5 to 35 degrees C, suggesting close connections between these lipid phases. Data concerning the identity and structural and functional roles of different lipid phases will be presented in the accompanying paper.
ER  -

DLOUHY, O., U. JAVORNIK, O. ZSIROS, P. SKET, V. KARLICKY, V. SPUNDA, J. PLAVEC a G. GARAB. Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy. \textit{Blood Cells, Molecules and Diseases}. Orlando, Florida: Academic Press, 2021, roč.~10, č.~9, s.~2354-2369. ISSN~1079-9796. Dostupné z: https://dx.doi.org/10.3390/cells10092354.

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