TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

D 2021

TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ, I. KRATOCHVILOVA et. al.

Základní údaje

Originální název

TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES

Autoři

PŘIBYL, Jan (203 Česká republika, domácí), Vladimír ROTREKL (203 Česká republika, domácí), Martin PEŠL (203 Česká republika, domácí), Šárka JELÍNKOVÁ (203 Česká republika, domácí) a I. KRATOCHVILOVA

Vydání

SLEZSKA, CONFERENCE PROCEEDINGS - NANOCON 2020, od s. 416-421, 6 s. 2021

Nakladatel

TANGER LTD

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10601 Cell biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14740/21:00120237

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-87294-98-7

ISSN

DOI

http://dx.doi.org/10.37904/nanocon.2020.3740

UT WoS

000664505500071

Klíčová slova anglicky

Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 3. 2022 13:41, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after thawing; they, when the dynamics of development of the cell stiffness were monitored in time up to 24 hours. Moreover, the AFM spectroscopy was combined with fluorescence-based staining of the cytoskeleton, thus enabling to directly correlate cytoskeleton development with stiffness mapping.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

NU20-06-00156, projekt VaV

Název: Vliv pneumologické medikace na funkce lidských kardiomyocytů.

Investor: Ministerstvo zdravotnictví ČR, Vliv pneumologické medikace na funkce lidských kardiomyocytů, Podprogram 1 - standardní

Citovat

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ a I. KRATOCHVILOVA. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In CONFERENCE PROCEEDINGS - NANOCON 2020. SLEZSKA: TANGER LTD, 2021, s. 416-421. ISBN 978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3740.

@inproceedings{1842662,
   author = {Přibyl, Jan and Rotrekl, Vladimír and Pešl, Martin and Jelínková, Šárka and Kratochvilova, I.},
   address = {SLEZSKA},
   booktitle = {CONFERENCE PROCEEDINGS - NANOCON 2020},
   doi = {http://dx.doi.org/10.37904/nanocon.2020.3740},
   keywords = {Atomic Force Microscopy; Mechanical Mapping; Cell stiffness; Cryopreservation},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {SLEZSKA},
   isbn = {978-80-87294-98-7},
   pages = {416-421},
   publisher = {TANGER LTD},
   title = {TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES},
   url = {https://www.confer.cz/nanocon/2020},
   year = {2021}
}

TY  - JOUR
ID  - 1842662
AU  - Přibyl, Jan - Rotrekl, Vladimír - Pešl, Martin - Jelínková, Šárka - Kratochvilova, I.
PY  - 2021
TI  - TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES
PB  - TANGER LTD
CY  - SLEZSKA
SN  - 9788087294987
KW  - Atomic Force Microscopy
KW  - Mechanical Mapping
KW  - Cell stiffness
KW  - Cryopreservation
UR  - https://www.confer.cz/nanocon/2020
N2  - Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM. Cell stiffness was mapped and thus monitored in time and space under nearly physiological conditions (i.e., in culture medium and at elevated temperature). In AFM force spectroscopy mode, cells are indented at many sites, and their complete elastic responses are recorded, enabling them to reconstruct a stiffness map. We measured the frozen cell surface stiffness immediately after thawing; they, when the dynamics of development of the cell stiffness were monitored in time up to 24 hours. Moreover, the AFM spectroscopy was combined with fluorescence-based staining of the cytoskeleton, thus enabling to directly correlate cytoskeleton development with stiffness mapping.
ER  -

PŘIBYL, Jan, Vladimír ROTREKL, Martin PEŠL, Šárka JELÍNKOVÁ a I. KRATOCHVILOVA. TIME-LAPSE MONITORING OF CELL MECHANICAL PROPERTIES. Online. In \textit{CONFERENCE PROCEEDINGS - NANOCON 2020}. SLEZSKA: TANGER LTD, 2021, s.~416-421. ISBN~978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3740.

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