COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS
AS BETTER SURFACE FOR CELL CULTURE

D 2021

COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE

KLIMOVIČ, Šimon, Jan PŘIBYL, Vladimír ROTREKL, Šárka JELÍNKOVÁ, H. VICAROVA et. al.

Základní údaje

Originální název

COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE

Autoři

KLIMOVIČ, Šimon (203 Česká republika, domácí), Jan PŘIBYL (203 Česká republika, domácí), Vladimír ROTREKL (203 Česká republika, domácí), Šárka JELÍNKOVÁ (203 Česká republika, domácí) a H. VICAROVA

Vydání

SLEZSKA, CONFERENCE PROCEEDINGS - NANOCON 2020, od s. 366-372, 7 s. 2021

Nakladatel

TANGER LTD

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10601 Cell biology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14740/21:00124449

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-87294-98-7

ISSN

DOI

http://dx.doi.org/10.37904/nanocon.2020.3739

UT WoS

000664505500062

Klíčová slova anglicky

AFM; Hydrogel; Actin structure; hyaluronic acid

Štítky

CF NANO, rivok

Změněno: 23. 3. 2022 13:46, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The mechanical and biomechanical properties of the cell's extracellular matrix is a factor in their later development. In this regard, elastic biocompatible materials as a surface for cultivating cells are in recent years, becoming an exciting field of study. Hydrogels are a hydrophilic 3D network of synthetic polymers or biopolymers. They can have tunable mechanical properties, controllable degradability, or they can be designed for long-time release of treatments. Here, hydrogels from Hyaluronic acid (HA), Bovine serum albumin (BSA) and gelatin was prepared, via carbodiimide chemistry. Morphology and mechanical properties of these hydrogels was characterized via Atomic Force Microscopy (AFM). Young's modulus of HA/BSA and HA/BSA/gel were 124.7 +/- 15.9 and 20.3 +/- 0.5 kPa, respectively. Mouse embryonic fibroblasts were then cultivated on hydrogels and on the glass surface as a reference. Mechanical and morphological properties of fibroblasts were examined by AFM and via phalloidin-TRITC staying. Relationship between the structure of cells and the type of surface was seen. Cells grown on glass were more flattened with an evenly linear actin structure. On hydrogels, fibroblasts were more star-shaped with non-linear actin structures and many filopodia. Furthermore, height of cells was higher was lower compared to cells on both types of hydrogels (0.36 mu m vs. 0.56 mu m vs. 1.03 mu m). Young's modulus of cells on fibroblast was highest, which relates to the whole range of structural changes linked with surface stiffness. In conclusion, this type of hydrogel might be utilized in the future as a better surface material for cell cultivation.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

Citovat

KLIMOVIČ, Šimon, Jan PŘIBYL, Vladimír ROTREKL, Šárka JELÍNKOVÁ a H. VICAROVA. COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE. Online. In CONFERENCE PROCEEDINGS - NANOCON 2020. SLEZSKA: TANGER LTD, 2021, s. 366-372. ISBN 978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3739.

@inproceedings{1842664,
   author = {Klimovič, Šimon and Přibyl, Jan and Rotrekl, Vladimír and Jelínková, Šárka and Vicarova, H.},
   address = {SLEZSKA},
   booktitle = {CONFERENCE PROCEEDINGS - NANOCON 2020},
   doi = {http://dx.doi.org/10.37904/nanocon.2020.3739},
   keywords = {AFM; Hydrogel; Actin structure; hyaluronic acid},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {SLEZSKA},
   isbn = {978-80-87294-98-7},
   pages = {366-372},
   publisher = {TANGER LTD},
   title = {COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE},
   url = {https://www.confer.cz/nanocon/2020},
   year = {2021}
}

TY  - JOUR
ID  - 1842664
AU  - Klimovič, Šimon - Přibyl, Jan - Rotrekl, Vladimír - Jelínková, Šárka - Vicarova, H.
PY  - 2021
TI  - COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE
PB  - TANGER LTD
CY  - SLEZSKA
SN  - 9788087294987
KW  - AFM
KW  - Hydrogel
KW  - Actin structure
KW  - hyaluronic acid
UR  - https://www.confer.cz/nanocon/2020
N2  - The mechanical and biomechanical properties of the cell's extracellular matrix is a factor in their later development. In this regard, elastic biocompatible materials as a surface for cultivating cells are in recent years, becoming an exciting field of study. Hydrogels are a hydrophilic 3D network of synthetic polymers or biopolymers. They can have tunable mechanical properties, controllable degradability, or they can be designed for long-time release of treatments. Here, hydrogels from Hyaluronic acid (HA), Bovine serum albumin (BSA) and gelatin was prepared, via carbodiimide chemistry. Morphology and mechanical properties of these hydrogels was characterized via Atomic Force Microscopy (AFM). Young's modulus of HA/BSA and HA/BSA/gel were 124.7 +/- 15.9 and 20.3 +/- 0.5 kPa, respectively. Mouse embryonic fibroblasts were then cultivated on hydrogels and on the glass surface as a reference. Mechanical and morphological properties of fibroblasts were examined by AFM and via phalloidin-TRITC staying. Relationship between the structure of cells and the type of surface was seen. Cells grown on glass were more flattened with an evenly linear actin structure. On hydrogels, fibroblasts were more star-shaped with non-linear actin structures and many filopodia. Furthermore, height of cells was higher was lower compared to cells on both types of hydrogels (0.36 mu m vs. 0.56 mu m vs. 1.03 mu m). Young's modulus of cells on fibroblast was highest, which relates to the whole range of structural changes linked with surface stiffness. In conclusion, this type of hydrogel might be utilized in the future as a better surface material for cell cultivation.
ER  -

KLIMOVIČ, Šimon, Jan PŘIBYL, Vladimír ROTREKL, Šárka JELÍNKOVÁ a H. VICAROVA. COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE. Online. In \textit{CONFERENCE PROCEEDINGS - NANOCON 2020}. SLEZSKA: TANGER LTD, 2021, s.~366-372. ISBN~978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3739.

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