BIO-APPLICABLE METAL NANOPARTICLES

D 2021

BIO-APPLICABLE METAL NANOPARTICLES

VYKOUKAL, Vít, Radka DOPITOVÁ, Martin POLÁK a Jiří NOVÁČEK

Základní údaje

Originální název

BIO-APPLICABLE METAL NANOPARTICLES

Autoři

VYKOUKAL, Vít (203 Česká republika, domácí), Radka DOPITOVÁ (203 Česká republika, domácí), Martin POLÁK (203 Česká republika, domácí) a Jiří NOVÁČEK (203 Česká republika, domácí)

Vydání

SLEZSKA, CONFERENCE PROCEEDINGS - NANOCON 2020, od s. 347-354, 8 s. 2021

Nakladatel

TANGER LTD

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10300 1.3 Physical sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Kód RIV

RIV/00216224:14740/21:00120018

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-87294-98-7

ISSN

DOI

http://dx.doi.org/10.37904/nanocon.2020.3736

UT WoS

000664505500059

Klíčová slova anglicky

Apoferritin; metal nanoparticles; cryo-electron microscopy

Štítky

CF CRYO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 14:40, Ing. Martina Blahová

Anotace

V originále

Metal nanoparticles find wide application potential in the biological research due to their unique optical, magnetic, electrical properties. Utilization of the nanoparticles prepared by chemical synthesis for biological research is limited by its solubility in water and toxicity. One potential solution to overcome this limitation is encapsulation of metal nanoparticles into protein or lipidic shell. Apoferritin is highly symmetric 12 nm protein cage composed of 24 apoferritin monomers which forms hollow structure with 8 nm cavity. Protein shell specifically interacts with the receptor on the cell membrane which facilitates uptake of the apoferritin from the extra-cellular environment into the cell. Due to the high stability of the protein cage and inherent capacity to encompass metal nanoparticles, apoferritin is widely focused as a potentionally optimal and general system for delivery of the metal nanoparticles into the organism. In addition, apoferritin can be also used as a nanoreactor for the nanoparticle production. We have generated a set of different nanoparticles encapsulated in apoferritin cage which we have intended to use as a label for structural and cellular biology research by cryo-electron microscopy. Here, we show that despite the 8 nm cavity, single 6-8 nm nanoparticle is not formed inside apoferritin cage. Instead, larger number of 2 nm or smaller nanoparticles is present inside apoferritin reducing overall number of atoms which can be incorporated into the molecule and thus the attainable contrast during electron microscopy imaging. Despite its wide utilization in life-science research, we conclude, that apoferritin derived nanoparticle system is not an optimal labeling probe for cryo-electron microscopy.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

TN01000008, projekt VaV

Název: Centrum elektronové a fotonové optiky

Investor: Technologická agentura ČR, Centrum elektronové a fotonové optiky

Citovat

VYKOUKAL, Vít, Radka DOPITOVÁ, Martin POLÁK a Jiří NOVÁČEK. BIO-APPLICABLE METAL NANOPARTICLES. Online. In CONFERENCE PROCEEDINGS - NANOCON 2020. SLEZSKA: TANGER LTD, 2021, s. 347-354. ISBN 978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3736.

@inproceedings{1842666,
   author = {Vykoukal, Vít and Dopitová, Radka and Polák, Martin and Nováček, Jiří},
   address = {SLEZSKA},
   booktitle = {CONFERENCE PROCEEDINGS - NANOCON 2020},
   doi = {http://dx.doi.org/10.37904/nanocon.2020.3736},
   keywords = {Apoferritin; metal nanoparticles; cryo-electron microscopy},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {SLEZSKA},
   isbn = {978-80-87294-98-7},
   pages = {347-354},
   publisher = {TANGER LTD},
   title = {BIO-APPLICABLE METAL NANOPARTICLES},
   url = {https://www.confer.cz/nanocon/2020},
   year = {2021}
}

TY  - JOUR
ID  - 1842666
AU  - Vykoukal, Vít - Dopitová, Radka - Polák, Martin - Nováček, Jiří
PY  - 2021
TI  - BIO-APPLICABLE METAL NANOPARTICLES
PB  - TANGER LTD
CY  - SLEZSKA
SN  - 9788087294987
KW  - Apoferritin
KW  - metal nanoparticles
KW  - cryo-electron microscopy
UR  - https://www.confer.cz/nanocon/2020
N2  - Metal nanoparticles find wide application potential in the biological research due to their unique optical, magnetic, electrical properties. Utilization of the nanoparticles prepared by chemical synthesis for biological research is limited by its solubility in water and toxicity. One potential solution to overcome this limitation is encapsulation of metal nanoparticles into protein or lipidic shell. Apoferritin is highly symmetric 12 nm protein cage composed of 24 apoferritin monomers which forms hollow structure with 8 nm cavity. Protein shell specifically interacts with the receptor on the cell membrane which facilitates uptake of the apoferritin from the extra-cellular environment into the cell. Due to the high stability of the protein cage and inherent capacity to encompass metal nanoparticles, apoferritin is widely focused as a potentionally optimal and general system for delivery of the metal nanoparticles into the organism. In addition, apoferritin can be also used as a nanoreactor for the nanoparticle production. We have generated a set of different nanoparticles encapsulated in apoferritin cage which we have intended to use as a label for structural and cellular biology research by cryo-electron microscopy. Here, we show that despite the 8 nm cavity, single 6-8 nm nanoparticle is not formed inside apoferritin cage. Instead, larger number of 2 nm or smaller nanoparticles is present inside apoferritin reducing overall number of atoms which can be incorporated into the molecule and thus the attainable contrast during electron microscopy imaging. Despite its wide utilization in life-science research, we conclude, that apoferritin derived nanoparticle system is not an optimal labeling probe for cryo-electron microscopy.
ER  -

VYKOUKAL, Vít, Radka DOPITOVÁ, Martin POLÁK a Jiří NOVÁČEK. BIO-APPLICABLE METAL NANOPARTICLES. Online. In \textit{CONFERENCE PROCEEDINGS - NANOCON 2020}. SLEZSKA: TANGER LTD, 2021, s.~347-354. ISBN~978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3736.

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