Functional expression and characterization of two laccases from
the brown rot Fomitopsis pinicola

J 2021

Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola

CSARMAN, F., T. OBERMANN, M.C. ZANJKO, P. MAN, P. HALADA et. al.

Základní údaje

Originální název

Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola

Autoři

CSARMAN, F., T. OBERMANN, M.C. ZANJKO, P. MAN, P. HALADA, B. SEIBOTH a R. LUDWIG

Vydání

ENZYME AND MICROBIAL TECHNOLOGY, 2021, 0141-0229

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.705

Kód RIV

RIV/00216224:14740/21:00124503

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.enzmictec.2021.109801

UT WoS

000663552500001

Klíčová slova anglicky

Biomass degradationBrown rotFomitopsis pinicolaLaccaseLigninTrichoderma reesei

Štítky

ne MU, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 5. 2022 15:03, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Laccase is predominantly found in lignin degrading filamentous white rot fungi, where it is involved in the oxidative degradation of this recalcitrant heteropolymer. In brown rot fungi it is much less prevalent: laccases from only a few brown rots have been detected and only two have been characterized. This study tries to understand the role of this ligninolytic enzyme in brown rots by investigating the catalytic properties of laccases secreted by Fomitopsis pinicola FP58527 SS1. When grown on either poplar or spruce wood blocks, several laccases were detected in the secretome. Two of them (FpLcc1 and FpLcc2) were heterologously produced using Trichoderma reesei QM9414 Delta xyr1 as expression host and purified to homogeneity by consecutive steps of hydrophobic interaction, anion exchange and size exclusion chromatography. With the substrates 2,2-azino-bis(3ethylthiazoline-6-sulfonate) (ABTS), 2,6-dimethoxyphenol (2,6-DMP) and guaiacol both laccases showed similar, low pH-optima below 3 for ABTS and 2,6-DMP and at pH 3.5 for guaiacol which is at the acidic end of laccases isolated from white rot fungi. The determined KM values were low while kcat values measured at acidic conditions were comparable to those reported for other laccases from white rot fungi. While both enzymes showed a moderate decrease in activity in the presence of oxalic and citric acid FpLcc2 was activated by acetic acid up to 3.7 times. This activation effect is much more pronounced at pH 5.0 compared to pH 3.0 and could already be observed at a concentration of 1 mM acetic acid.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

CSARMAN, F., T. OBERMANN, M.C. ZANJKO, P. MAN, P. HALADA, B. SEIBOTH a R. LUDWIG. Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola. ENZYME AND MICROBIAL TECHNOLOGY. 2021, roč. 148, AUG, s. 109801-109812. ISSN 0141-0229. Dostupné z: https://dx.doi.org/10.1016/j.enzmictec.2021.109801.

@article{1845723,
   author = {Csarman, F. and Obermann, T. and Zanjko, M.C. and Man, P. and Halada, P. and Seiboth, B. and Ludwig, R.},
   article_number = {AUG},
   doi = {http://dx.doi.org/10.1016/j.enzmictec.2021.109801},
   keywords = {Biomass degradationBrown rotFomitopsis pinicolaLaccaseLigninTrichoderma reesei},
   language = {eng},
   issn = {0141-0229},
   journal = {ENZYME AND MICROBIAL TECHNOLOGY},
   title = {Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola},
   url = {https://www.sciencedirect.com/science/article/pii/S0141022921000594?via%3Dihub},
   volume = {148},
   year = {2021}
}

TY  - JOUR
ID  - 1845723
AU  - Csarman, F. - Obermann, T. - Zanjko, M.C. - Man, P. - Halada, P. - Seiboth, B. - Ludwig, R.
PY  - 2021
TI  - Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola
JF  - ENZYME AND MICROBIAL TECHNOLOGY
VL  - 148
IS  - AUG
SP  - 109801
EP  - 109801
SN  - 01410229
KW  - Biomass degradationBrown rotFomitopsis pinicolaLaccaseLigninTrichoderma reesei
UR  - https://www.sciencedirect.com/science/article/pii/S0141022921000594?via%3Dihub
N2  - Laccase is predominantly found in lignin degrading filamentous white rot fungi, where it is involved in the oxidative degradation of this recalcitrant heteropolymer. In brown rot fungi it is much less prevalent: laccases from only a few brown rots have been detected and only two have been characterized. This study tries to understand the role of this ligninolytic enzyme in brown rots by investigating the catalytic properties of laccases secreted by Fomitopsis pinicola FP58527 SS1. When grown on either poplar or spruce wood blocks, several laccases were detected in the secretome. Two of them (FpLcc1 and FpLcc2) were heterologously produced using Trichoderma reesei QM9414 Delta xyr1 as expression host and purified to homogeneity by consecutive steps of hydrophobic interaction, anion exchange and size exclusion chromatography. With the substrates 2,2-azino-bis(3ethylthiazoline-6-sulfonate) (ABTS), 2,6-dimethoxyphenol (2,6-DMP) and guaiacol both laccases showed similar, low pH-optima below 3 for ABTS and 2,6-DMP and at pH 3.5 for guaiacol which is at the acidic end of laccases isolated from white rot fungi. The determined KM values were low while kcat values measured at acidic conditions were comparable to those reported for other laccases from white rot fungi. While both enzymes showed a moderate decrease in activity in the presence of oxalic and citric acid FpLcc2 was activated by acetic acid up to 3.7 times. This activation effect is much more pronounced at pH 5.0 compared to pH 3.0 and could already be observed at a concentration of 1 mM acetic acid.
ER  -

CSARMAN, F., T. OBERMANN, M.C. ZANJKO, P. MAN, P. HALADA, B. SEIBOTH a R. LUDWIG. Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola. \textit{ENZYME AND MICROBIAL TECHNOLOGY}. 2021, roč.~148, AUG, s.~109801-109812. ISSN~0141-0229. Dostupné z: https://dx.doi.org/10.1016/j.enzmictec.2021.109801.

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