Další formáty:
BibTeX
LaTeX
RIS
@article{1846838, author = {Hnizda, A. and Tesina, P. and Nguyen, T.B. and Kukacka, Z. and Kater, L. and Chaplin, A. and Beckmann, R. and Ascher, D.B. and Novak, P. and Blundell, T.L.}, article_number = {14}, doi = {http://dx.doi.org/10.1111/febs.15732}, keywords = {DNA double‐strand breakintegrative structural biologyKu7080nonhomologous end joiningSAP domain}, language = {eng}, issn = {1742-464X}, journal = {FEBS JOURNAL}, title = {SAP domain forms a flexible part of DNA aperture in Ku70/80}, url = {https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15732}, volume = {288}, year = {2021} }
TY - JOUR ID - 1846838 AU - Hnizda, A. - Tesina, P. - Nguyen, T.B. - Kukacka, Z. - Kater, L. - Chaplin, A. - Beckmann, R. - Ascher, D.B. - Novak, P. - Blundell, T.L. PY - 2021 TI - SAP domain forms a flexible part of DNA aperture in Ku70/80 JF - FEBS JOURNAL VL - 288 IS - 14 SP - 4382-4393 EP - 4382-4393 SN - 1742464X KW - DNA double‐strand breakintegrative structural biologyKu7080nonhomologous end joiningSAP domain UR - https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15732 N2 - Nonhomologous end joining (NHEJ) is a DNA repair mechanism that religates double-strand DNA breaks to maintain genomic integrity during the entire cell cycle. The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. Here, we describe intramolecular interactions of the Ku70 C-terminal domain, known as the SAP domain. Using single-particle cryo-electron microscopy, mass spectrometric analysis of intermolecular cross-linking and molecular modelling simulations, we captured variable positions of the SAP domain depending on DNA binding. The first position was localized at the DNA aperture in the Ku70/80 apo form but was not observed in the DNA-bound state. The second position, which was observed in both apo and DNA-bound states, was found below the DNA aperture, close to the helical arm of Ku70. The localization of the SAP domain in the DNA aperture suggests a function as a flexible entry gate for broken DNA. Databases EM maps have been deposited in EMDB (EMD-11933). Coordinates have been deposited in Protein Data Bank (PDB ). Other data are available from corresponding authors upon a request. ER -
HNIZDA, A., P. TESINA, T.B. NGUYEN, Z. KUKACKA, L. KATER, A. CHAPLIN, R. BECKMANN, D.B. ASCHER, P. NOVAK a T.L. BLUNDELL. SAP domain forms a flexible part of DNA aperture in Ku70/80. \textit{FEBS JOURNAL}. 2021, roč.~288, č.~14, s.~4382-4393. ISSN~1742-464X. Dostupné z: https://dx.doi.org/10.1111/febs.15732.
|