PRE-FORMULATION DESIGN OF SUSTAINED-RELEASE GnRHa-LOADED PLGA
MICROSPHERES AND ASSOCIATED FORMULATIONS FOR CONTROLLING
REPRODUCTION IN AQUACULTURE

J 2021

PRE-FORMULATION DESIGN OF SUSTAINED-RELEASE GnRHa-LOADED PLGA MICROSPHERES AND ASSOCIATED FORMULATIONS FOR CONTROLLING REPRODUCTION IN AQUACULTURE

HOLICKÁ, Martina, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Jan MUSELÍK, Eva RADINOVÁ et. al.

Basic information

Original name

PRE-FORMULATION DESIGN OF SUSTAINED-RELEASE GnRHa-LOADED PLGA MICROSPHERES AND ASSOCIATED FORMULATIONS FOR CONTROLLING REPRODUCTION IN AQUACULTURE

Authors

HOLICKÁ, Martina (203 Czech Republic, belonging to the institution), Jakub VYSLOUŽIL (203 Czech Republic, belonging to the institution), Kateřina KUBOVÁ (203 Czech Republic, guarantor, belonging to the institution), Jan MUSELÍK (203 Czech Republic, belonging to the institution), Eva RADINOVÁ (203 Czech Republic, belonging to the institution), David VETCHÝ (203 Czech Republic, belonging to the institution), Hana KOTOLOVÁ (203 Czech Republic, belonging to the institution), Tomáš HAMMER (203 Czech Republic, belonging to the institution), J. MAŠEK, P. PODHOREC and J. KNOWLES

Edition

ACTA POLONIAE PHARMACEUTICA, Warsaw, POLSKIE TOWARZYSTWO FARMACEUTYCZNE, 2021, 0001-6837

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.555

RIV identification code

RIV/00216224:14160/21:00119773

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.32383/appdr/146287

UT WoS

000772786500009

Keywords in English

GnRH analogsmicroparticlessolvent evaporationgelatinesustained drug releasefish reproduction

Abstract

V originále

Poly(lactide-co-glycolide) PLGA microparticles represent an efficient and modern tool to encapsulate peptide drugs, which enables their administration to live organisms with the benefit of prolongedrelease. One area that could make the best of this opportunity is fish breeding in aquaculture. The presented study was centered on the formulation of gonadotropin-releasing hormone (GnRH) analog loaded PLGA microparticles intended for fish breeding augmentation, using the double emulsion evaporation method. In this initial experiment, the influence of several input variables (drug, PLGA type, emulsifier concentration, gelatine concentration in internal phase) on observed parameters (morphology, particle size, drug content, drug release, resuspension index) was evaluated. It was found that at lower emulsifier concentration, the particle size is apparently lower (8.54 +/- 6.13-10.36 +/- 4.65 vs. 25.56 +/- 18.86), which is more advantageous in injection administration. Encapsulation efficiency ranged from 39.13 +/- 8.85 to 75.30 +/- 8.83, favoring lower emulsifier concentration, while resuspension index (70.99 +/- 6.47%) suggested the possibility of longer-term administration. Dissolution tests revealed prolonged release for ten days, with most of the drug released in 72-96 hrs. A follow-up study discerning polymer type/gelatine concentration was suggested. Accompanying studies of imaging agent coumarin-6 encapsulation for eventual distribution imaging and metoclopramide base drug release for potential adjuvant use were also successfully realized.

Links

QK1810221, research and development project

Name: Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.

Investor: Ministry of Agriculture of the CR

Citovat

HOLICKÁ, Martina, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Jan MUSELÍK, Eva RADINOVÁ, David VETCHÝ, Hana KOTOLOVÁ, Tomáš HAMMER, J. MAŠEK, P. PODHOREC and J. KNOWLES. PRE-FORMULATION DESIGN OF SUSTAINED-RELEASE GnRHa-LOADED PLGA MICROSPHERES AND ASSOCIATED FORMULATIONS FOR CONTROLLING REPRODUCTION IN AQUACULTURE. ACTA POLONIAE PHARMACEUTICA. Warsaw: POLSKIE TOWARZYSTWO FARMACEUTYCZNE, 2021, vol. 78, No 6, p. 801-812. ISSN 0001-6837. Available from: https://dx.doi.org/10.32383/appdr/146287.

@article{1848069,
   author = {Holická, Martina and Vysloužil, Jakub and Kubová, Kateřina and Muselík, Jan and Radinová, Eva and Vetchý, David and Kotolová, Hana and Hammer, Tomáš and Mašek, J. and Podhorec, P. and Knowles, J.},
   article_location = {Warsaw},
   article_number = {6},
   doi = {http://dx.doi.org/10.32383/appdr/146287},
   keywords = {GnRH analogsmicroparticlessolvent evaporationgelatinesustained drug releasefish reproduction},
   language = {eng},
   issn = {0001-6837},
   journal = {ACTA POLONIAE PHARMACEUTICA},
   title = {PRE-FORMULATION DESIGN OF SUSTAINED-RELEASE GnRHa-LOADED PLGA MICROSPHERES AND ASSOCIATED FORMULATIONS FOR CONTROLLING REPRODUCTION IN AQUACULTURE},
   url = {https://www.ptfarm.pl/download/?file=File%2FActa_Poloniae%2F2021%2F6%2F801.pdf},
   volume = {78},
   year = {2021}
}

TY  - JOUR
ID  - 1848069
AU  - Holická, Martina - Vysloužil, Jakub - Kubová, Kateřina - Muselík, Jan - Radinová, Eva - Vetchý, David - Kotolová, Hana - Hammer, Tomáš - Mašek, J. - Podhorec, P. - Knowles, J.
PY  - 2021
TI  - PRE-FORMULATION DESIGN OF SUSTAINED-RELEASE GnRHa-LOADED PLGA MICROSPHERES AND ASSOCIATED FORMULATIONS FOR CONTROLLING REPRODUCTION IN AQUACULTURE
JF  - ACTA POLONIAE PHARMACEUTICA
VL  - 78
IS  - 6
SP  - 801-812
EP  - 801-812
PB  - POLSKIE TOWARZYSTWO FARMACEUTYCZNE
SN  - 00016837
KW  - GnRH analogsmicroparticlessolvent evaporationgelatinesustained drug releasefish reproduction
UR  - https://www.ptfarm.pl/download/?file=File%2FActa_Poloniae%2F2021%2F6%2F801.pdf
N2  - Poly(lactide-co-glycolide) PLGA microparticles represent an efficient and modern tool to encapsulate peptide drugs, which enables their administration to live organisms with the benefit of prolongedrelease. One area that could make the best of this opportunity is fish breeding in aquaculture. The presented study was centered on the formulation of gonadotropin-releasing hormone (GnRH) analog loaded PLGA microparticles intended for fish breeding augmentation, using the double emulsion evaporation method. In this initial experiment, the influence of several input variables (drug, PLGA type, emulsifier concentration, gelatine concentration in internal phase) on observed parameters (morphology, particle size, drug content, drug release, resuspension index) was evaluated. It was found that at lower emulsifier concentration, the particle size is apparently lower (8.54 +/- 6.13-10.36 +/- 4.65 vs. 25.56 +/- 18.86), which is more advantageous in injection administration. Encapsulation efficiency ranged from 39.13 +/- 8.85 to 75.30 +/- 8.83, favoring lower emulsifier concentration, while resuspension index (70.99 +/- 6.47%) suggested the possibility of longer-term administration. Dissolution tests revealed prolonged release for ten days, with most of the drug released in 72-96 hrs. A follow-up study discerning polymer type/gelatine concentration was suggested. Accompanying studies of imaging agent coumarin-6 encapsulation for eventual distribution imaging and metoclopramide base drug release for potential adjuvant use were also successfully realized.
ER  -

HOLICKÁ, Martina, Jakub VYSLOUŽIL, Kateřina KUBOVÁ, Jan MUSELÍK, Eva RADINOVÁ, David VETCHÝ, Hana KOTOLOVÁ, Tomáš HAMMER, J. MAŠEK, P. PODHOREC and J. KNOWLES. PRE-FORMULATION DESIGN OF SUSTAINED-RELEASE GnRHa-LOADED PLGA MICROSPHERES AND ASSOCIATED FORMULATIONS FOR CONTROLLING REPRODUCTION IN AQUACULTURE. \textit{ACTA POLONIAE PHARMACEUTICA}. Warsaw: POLSKIE TOWARZYSTWO FARMACEUTYCZNE, 2021, vol.~78, No~6, p.~801-812. ISSN~0001-6837. Available from: https://dx.doi.org/10.32383/appdr/146287.

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