Viability of bovine in vitro matured oocytes following
ultra-rapid vitrification

J 2022

Viability of bovine in vitro matured oocytes following ultra-rapid vitrification

DUJÍČKOVÁ, Linda, Lucia OLEXIKOVÁ, Elena KUBOVIČOVÁ, Jiří BEZDÍČEK, Michal JEŠETA et. al.

Základní údaje

Originální název

Viability of bovine in vitro matured oocytes following ultra-rapid vitrification

Autoři

DUJÍČKOVÁ, Linda, Lucia OLEXIKOVÁ, Elena KUBOVIČOVÁ, Jiří BEZDÍČEK (203 Česká republika, garant), Michal JEŠETA (203 Česká republika, domácí) a Alexander MAKAREVICH

Vydání

Medical Journal of Cell Biology, Warwaw, Walter de Gruyter, 2022, 2544-3577

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/22:00125709

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.2478/acb-2022-0001

Klíčová slova anglicky

vitrification; bovine; oocyte; embryo; ultrastructure; apoptosis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 3. 2023 09:31, Mgr. Tereza Miškechová

Anotace

V originále

The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-ra-pid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P < 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.

Citovat

DUJÍČKOVÁ, Linda, Lucia OLEXIKOVÁ, Elena KUBOVIČOVÁ, Jiří BEZDÍČEK, Michal JEŠETA a Alexander MAKAREVICH. Viability of bovine in vitro matured oocytes following ultra-rapid vitrification. Medical Journal of Cell Biology. Warwaw: Walter de Gruyter, 2022, roč. 10, č. 1, s. 1-6. ISSN 2544-3577. Dostupné z: https://dx.doi.org/10.2478/acb-2022-0001.

@article{1849260,
   author = {Dujíčková, Linda and Olexiková, Lucia and Kubovičová, Elena and Bezdíček, Jiří and Ješeta, Michal and Makarevich, Alexander},
   article_location = {Warwaw},
   article_number = {1},
   doi = {http://dx.doi.org/10.2478/acb-2022-0001},
   keywords = {vitrification; bovine; oocyte; embryo; ultrastructure; apoptosis},
   language = {eng},
   issn = {2544-3577},
   journal = {Medical Journal of Cell Biology},
   title = {Viability of bovine in vitro matured oocytes following ultra-rapid vitrification},
   url = {https://sciendo.com/it/article/10.2478/acb-2022-0001},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 1849260
AU  - Dujíčková, Linda - Olexiková, Lucia - Kubovičová, Elena - Bezdíček, Jiří - Ješeta, Michal - Makarevich, Alexander
PY  - 2022
TI  - Viability of bovine in vitro matured oocytes following ultra-rapid vitrification
JF  - Medical Journal of Cell Biology
VL  - 10
IS  - 1
SP  - 1-6
EP  - 1-6
PB  - Walter de Gruyter
SN  - 25443577
KW  - vitrification
KW  - bovine
KW  - oocyte
KW  - embryo
KW  - ultrastructure
KW  - apoptosis
UR  - https://sciendo.com/it/article/10.2478/acb-2022-0001
N2  - The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-ra-pid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P < 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.
ER  -

DUJÍČKOVÁ, Linda, Lucia OLEXIKOVÁ, Elena KUBOVIČOVÁ, Jiří BEZDÍČEK, Michal JEŠETA a Alexander MAKAREVICH. Viability of bovine in vitro matured oocytes following ultra-rapid vitrification. \textit{Medical Journal of Cell Biology}. Warwaw: Walter de Gruyter, 2022, roč.~10, č.~1, s.~1-6. ISSN~2544-3577. Dostupné z: https://dx.doi.org/10.2478/acb-2022-0001.

Podrobný výpis o publikaci