| VÁCLAVEK, Tomáš a František FORET. Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds. Electrophoresis. Verlag Chemie, 2023, roč. 44, 1-2, s. 313-322. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.202100379. |
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@article{1850582,
author = {Václavek, Tomáš and Foret, František},
article_number = {1-2},
doi = {http://dx.doi.org/10.1002/elps.202100379},
keywords = {bioanalysis; hemoglobin; mass spectrometry; microfluidics; single-cell analysis},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds},
url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elps.202100379},
volume = {44},
year = {2023}
}
TY - JOUR
ID - 1850582
AU - Václavek, Tomáš - Foret, František
PY - 2023
TI - Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds
JF - Electrophoresis
VL - 44
IS - 1-2
SP - 313-322
EP - 313-322
PB - Verlag Chemie
SN - 01730835
KW - bioanalysis
KW - hemoglobin
KW - mass spectrometry
KW - microfluidics
KW - single-cell analysis
UR - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elps.202100379
N2 - Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates.
ER -
VÁCLAVEK, Tomáš a František FORET. Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds. \textit{Electrophoresis}. Verlag Chemie, 2023, roč.~44, 1-2, s.~313-322. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.202100379.
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