J 2022

Starter feed for carnivorous species as a practical replacement of bloodworms for a vertebrate model organism in ageing, the turquoise killifish Nothobranchius furzeri

ŽÁK, Jakub, Koushik ROY, Iva DYKOVÁ, Jan MRÁZ, Martin REICHARD et. al.

Základní údaje

Originální název

Starter feed for carnivorous species as a practical replacement of bloodworms for a vertebrate model organism in ageing, the turquoise killifish Nothobranchius furzeri

Autoři

ŽÁK, Jakub (garant), Koushik ROY, Iva DYKOVÁ (203 Česká republika, domácí), Jan MRÁZ a Martin REICHARD (203 Česká republika, domácí)

Vydání

Journal of Fish Biology, Wiley, 2022, 0022-1112

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10617 Marine biology, freshwater biology, limnology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 2.000

Kód RIV

RIV/00216224:14310/22:00125770

Organizační jednotka

Přírodovědecká fakulta

DOI

UT WoS

000771139200001

Klíčová slova anglicky

African killifish; laboratory diet; nutritional ecology; practical diet

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 9. 8. 2022 14:26, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The absence of a controlled diet is unfortunate in a promising model organism for ageing, the turquoise killifish (Nothobranchius furzeri Jubb, 1971). Currently captive N. furzeri are fed bloodworms but it is not known whether this is an optimal diet. Replacing bloodworms with a practical dry feed would reduce diet variability. In the present study, we estimated the nutritional value of the diet ingested by wild fish and determined the fish-body amino acid profile as a proxy for their nutritional requirements. We compared the performance of fish fed four commercial feeds containing 46%–64% protein to that achieved with bloodworms and that of wild fish. Wild fish target a high-protein (60%) diet and this is supported by their superior performance on high-protein diets in captivity. In contrast, feeds for omnivores led to slower growth, lower fecundity and unnatural liver size. In comparison to wild fish, a bloodworm diet led to lower body condition, overfeeding and male liver enlargement. Out of the four dry feeds tested, the fish fed Aller matched wild fish in body condition and liver size, and was comparable to bloodworms in terms of growth and fecundity. A starter feed for carnivorous species appears to be a practical replacement for bloodworms for N. furzeri. The use of dry feeds improved performance in comparison to bloodworms and thus may contribute to reducing response variability and improving research reproducibility in N. furzeri research.