Pedobacter fastidiosus sp. nov., isolated from glacial habitats
of maritime Antarctica

J 2022

Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

ŠVEC, Pavel, Stanislava KRÁLOVÁ, Eva STAŇKOVÁ, Pavla HOLOCHOVÁ, Karel SEDLÁŘ et. al.

Základní údaje

Originální název

Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica

Autoři

ŠVEC, Pavel (203 Česká republika, domácí), Stanislava KRÁLOVÁ (703 Slovensko, garant, domácí), Eva STAŇKOVÁ (203 Česká republika, domácí), Pavla HOLOCHOVÁ (203 Česká republika, domácí), Karel SEDLÁŘ, Sylva KOUDELKOVÁ (203 Česká republika, domácí), Daniel KRSEK, Jakub GRZESIAK, Ondrej ŠEDO (203 Česká republika, domácí), Peter VÁCZI (703 Slovensko, domácí), Urvashi SAH, Vipin GUPTA, Utkarsh SOOD, Rup LAL, Suresh KORPOLE a Ivo SEDLÁČEK (203 Česká republika, domácí)

Vydání

International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2022, 1466-5026

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.800

Kód RIV

RIV/00216224:14310/22:00125779

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1099/ijsem.0.005309

UT WoS

000800144400027

Klíčová slova anglicky

Pedobacter fastidiosus sp. nov.; proglacial stream; glacier; Antarctica; taxonomy

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 21:08, Ing. Martina Blahová

Anotace

V originále

Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island and King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies and exhibited identical 16S rRNA gene sequences and highly similar MALDI TOF mass spectra, and hence were assigned as representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences assigned both isolates to the genus Pedobacter and showed Pedobacter frigidisoli and Pedobacter terrae to be their closest phylogenetic neighbours, with 97.4 and 97.2 % 16S rRNA gene sequence similarities, respectively. These low similarity values were below the threshold similarity value of 98.7%, confirming the delineation of a new bacterial species. Further genomic characterization included whole-genome sequencing accompanied by average nucleotide identity (ANI) and digital DNA–DNA hybridization calculations, and characterization of the genome features. The ANI values between P8930T and P. frigidisoli RP-3-11T and P. terrae DSM 17933T were 79.7 and 77.6 %, respectively, and the value between P. frigidisoli RP-3-11T and P. terrae DSM 17933T was 77.7 %, clearly demonstrating the phylogenetic distance and the novelty of strain P8930T. Further characterization included analysis of cellular fatty acids, quinones and polar lipids, and comprehensive biotyping. All the obtained results proved the separation of strains P8930T and 478 from the other validly named Pedobacter species, and confirmed that they represent a new species for which the name Pedobacter fastidiosus sp. nov. is proposed. The type strain is P8930T (=CCM 8938T=LMG 32098T).

Návaznosti

EF16_013/0001708, projekt VaV

Název: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2015078, projekt VaV

Název: Česká polární výzkumná infrastruktura (Akronym: CzechPolar2)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar Research Infrastructure

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

ŠVEC, Pavel, Stanislava KRÁLOVÁ, Eva STAŇKOVÁ, Pavla HOLOCHOVÁ, Karel SEDLÁŘ, Sylva KOUDELKOVÁ, Daniel KRSEK, Jakub GRZESIAK, Ondrej ŠEDO, Peter VÁCZI, Urvashi SAH, Vipin GUPTA, Utkarsh SOOD, Rup LAL, Suresh KORPOLE a Ivo SEDLÁČEK. Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica. International Journal of Systematic and Evolutionary Microbiology. Microbiology Society, 2022, roč. 72, č. 4, s. 1-9. ISSN 1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.005309.

@article{1851997,
   author = {Švec, Pavel and Králová, Stanislava and Staňková, Eva and Holochová, Pavla and Sedlář, Karel and Koudelková, Sylva and Krsek, Daniel and Grzesiak, Jakub and Šedo, Ondrej and Váczi, Peter and Sah, Urvashi and Gupta, Vipin and Sood, Utkarsh and Lal, Rup and Korpole, Suresh and Sedláček, Ivo},
   article_number = {4},
   doi = {http://dx.doi.org/10.1099/ijsem.0.005309},
   keywords = {Pedobacter fastidiosus sp. nov.; proglacial stream; glacier; Antarctica; taxonomy},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica},
   url = {https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005309},
   volume = {72},
   year = {2022}
}

TY  - JOUR
ID  - 1851997
AU  - Švec, Pavel - Králová, Stanislava - Staňková, Eva - Holochová, Pavla - Sedlář, Karel - Koudelková, Sylva - Krsek, Daniel - Grzesiak, Jakub - Šedo, Ondrej - Váczi, Peter - Sah, Urvashi - Gupta, Vipin - Sood, Utkarsh - Lal, Rup - Korpole, Suresh - Sedláček, Ivo
PY  - 2022
TI  - Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 72
IS  - 4
SP  - 1-9
EP  - 1-9
PB  - Microbiology Society
SN  - 14665026
KW  - Pedobacter fastidiosus sp. nov.
KW  - proglacial stream
KW  - glacier
KW  - Antarctica
KW  - taxonomy
UR  - https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005309
N2  - Strains P8930T and 478 were isolated from Antarctic glaciers located on James Ross Island and King George Island, respectively. They comprised Gram-stain-negative short rod-shaped cells forming pink pigmented colonies and exhibited identical 16S rRNA gene sequences and highly similar MALDI TOF mass spectra, and hence were assigned as representatives of the same species. Phylogenetic analysis based on 16S rRNA gene sequences assigned both isolates to the genus Pedobacter and showed Pedobacter frigidisoli and Pedobacter terrae to be their closest phylogenetic neighbours, with 97.4 and 97.2 % 16S rRNA gene sequence similarities, respectively. These low similarity values were below the threshold similarity value of 98.7%, confirming the delineation of a new bacterial species. Further genomic characterization included whole-genome sequencing accompanied by average nucleotide identity (ANI) and digital DNA–DNA hybridization calculations, and characterization of the genome features. The ANI values between P8930T and P. frigidisoli RP-3-11T and P. terrae DSM 17933T were 79.7 and 77.6 %, respectively, and the value between P. frigidisoli RP-3-11T and P. terrae DSM 17933T was 77.7 %, clearly demonstrating the phylogenetic distance and the novelty of strain P8930T. Further characterization included analysis of cellular fatty acids, quinones and polar lipids, and comprehensive biotyping. All the obtained results proved the separation of strains P8930T and 478 from the other validly named Pedobacter species, and confirmed that they represent a new species for which the name Pedobacter fastidiosus sp. nov. is proposed. The type strain is P8930T (=CCM 8938T=LMG 32098T).
ER  -

ŠVEC, Pavel, Stanislava KRÁLOVÁ, Eva STAŇKOVÁ, Pavla HOLOCHOVÁ, Karel SEDLÁŘ, Sylva KOUDELKOVÁ, Daniel KRSEK, Jakub GRZESIAK, Ondrej ŠEDO, Peter VÁCZI, Urvashi SAH, Vipin GUPTA, Utkarsh SOOD, Rup LAL, Suresh KORPOLE a Ivo SEDLÁČEK. Pedobacter fastidiosus sp. nov., isolated from glacial habitats of maritime Antarctica. \textit{International Journal of Systematic and Evolutionary Microbiology}. Microbiology Society, 2022, roč.~72, č.~4, s.~1-9. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.005309.

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