Combination of Sildenafil and Ba2+ at a Low Concentration Show
a Significant Synergistic Inhibition of Inward Rectifier
Potassium Current Resulting in Action Potential Prolongation

J 2022

Combination of Sildenafil and Ba2+ at a Low Concentration Show a Significant Synergistic Inhibition of Inward Rectifier Potassium Current Resulting in Action Potential Prolongation

MACHÁČEK, Martin, Olga ŠVECOVÁ a Markéta BÉBAROVÁ

Základní údaje

Originální název

Combination of Sildenafil and Ba2+ at a Low Concentration Show a Significant Synergistic Inhibition of Inward Rectifier Potassium Current Resulting in Action Potential Prolongation

Autoři

MACHÁČEK, Martin (203 Česká republika, domácí), Olga ŠVECOVÁ (203 Česká republika, domácí) a Markéta BÉBAROVÁ (203 Česká republika, garant, domácí)

Vydání

Frontiers in Pharmacology, LAUSANNE, FRONTIERS MEDIA S, 2022, 1663-9812

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.600

Kód RIV

RIV/00216224:14110/22:00125819

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3389/fphar.2022.829952

UT WoS

000793832200001

Klíčová slova anglicky

arrhythmia; barium; cardiomyocytes; inward rectifier potassium current; sildenafil; synergy

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 7. 2022 08:40, Mgr. Tereza Miškechová

Anotace

V originále

Sildenafil (Viagra) is a vasodilator mainly used in the treatment of erectile dysfunction. Atrial or ventricular fibrillation may rarely occur as a side effect during sildenafil therapy. Although changes in inward rectifier potassium currents including IK1 are known to contribute to the pathogenesis of fibrillation, the effect of sildenafil on IK1 has not been studied. In experiments, Ba2+ is used as a specific inhibitor of IK1 at high concentrations (usually 100 µM). Being an environmental contaminant, it is also present in the human body; Ba2+ plasmatic concentrations up to 1.5 µM are usually reported in the general population. This study was primarily aimed to investigate changes of IK1 induced by sildenafil in a wide range of concentrations (0.1–100 µM). Additionally, the effect of combination of sildenafil and Ba2+ at selected clinically-relevant concentrations was tested, at 0.1 µM both on IK1 and on the action potential duration (APD). Experiments were performed by the whole-cell patch-clamp technique on enzymatically isolated rat ventricular cardiomyocytes, mostly at 23°C with the exception of APD measurements which were performed at 37°C as well. Sildenafil caused a significant, reversible, and concentration-dependent inhibition of IK1 that did not differ at −50 and −110 mV. Simultaneous application of sildenafil and Ba2+ at 0.1 µM revealed a massive inhibition of both inward and outward components of IK1 (this synergy was missing at other tested combinations). The combined effect at 0.1 µM (45.7 ± 5.7 and 43.0 ± 6.9% inhibition at −50 and −110 mV, respectively) was significantly higher than a simple sum of almost negligible effects of the individual substances and it led to a significant prolongation of APD at both 23 and 37°C. To our knowledge, similar potentiation of the drug-channel interaction has not been described. The observed massive inhibition of IK1 induced by a combined action of the vasodilator sildenafil and environmental contaminant Ba2+ at a low concentration and resulting in a significant APD prolongation may contribute to the genesis of arrhythmias observed in some patients treated with sildenafil.

Návaznosti

MUNI/A/1133/2021, interní kód MU

Název: Od buňky k medicíně (Akronym: CELLVIEW)

Investor: Masarykova univerzita, Od buňky k medicíně

Citovat

MACHÁČEK, Martin, Olga ŠVECOVÁ a Markéta BÉBAROVÁ. Combination of Sildenafil and Ba2+ at a Low Concentration Show a Significant Synergistic Inhibition of Inward Rectifier Potassium Current Resulting in Action Potential Prolongation. Frontiers in Pharmacology. LAUSANNE: FRONTIERS MEDIA S, 2022, roč. 13, April 2022, s. 1-9. ISSN 1663-9812. Dostupné z: https://dx.doi.org/10.3389/fphar.2022.829952.

@article{1853625,
   author = {Macháček, Martin and Švecová, Olga and Bébarová, Markéta},
   article_location = {LAUSANNE},
   article_number = {April 2022},
   doi = {http://dx.doi.org/10.3389/fphar.2022.829952},
   keywords = {arrhythmia; barium; cardiomyocytes; inward rectifier potassium current; sildenafil; synergy},
   language = {eng},
   issn = {1663-9812},
   journal = {Frontiers in Pharmacology},
   title = {Combination of Sildenafil and Ba2+ at a Low Concentration Show a Significant Synergistic Inhibition of Inward Rectifier Potassium Current Resulting in Action Potential Prolongation},
   url = {https://www.frontiersin.org/articles/10.3389/fphar.2022.829952/full},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 1853625
AU  - Macháček, Martin - Švecová, Olga - Bébarová, Markéta
PY  - 2022
TI  - Combination of Sildenafil and Ba2+ at a Low Concentration Show a Significant Synergistic Inhibition of Inward Rectifier Potassium Current Resulting in Action Potential Prolongation
JF  - Frontiers in Pharmacology
VL  - 13
IS  - April 2022
SP  - 1-9
EP  - 1-9
PB  - FRONTIERS MEDIA S
SN  - 16639812
KW  - arrhythmia
KW  - barium
KW  - cardiomyocytes
KW  - inward rectifier potassium current
KW  - sildenafil
KW  - synergy
UR  - https://www.frontiersin.org/articles/10.3389/fphar.2022.829952/full
N2  - Sildenafil (Viagra) is a vasodilator mainly used in the treatment of erectile dysfunction. Atrial or ventricular fibrillation may rarely occur as a side effect during sildenafil therapy. Although changes in inward rectifier potassium currents including IK1 are known to contribute to the pathogenesis of fibrillation, the effect of sildenafil on IK1 has not been studied. In experiments, Ba2+ is used as a specific inhibitor of IK1 at high concentrations (usually 100 µM). Being an environmental contaminant, it is also present in the human body; Ba2+ plasmatic concentrations up to 1.5 µM are usually reported in the general population. This study was primarily aimed to investigate changes of IK1 induced by sildenafil in a wide range of concentrations (0.1–100 µM). Additionally, the effect of combination of sildenafil and Ba2+ at selected clinically-relevant concentrations was tested, at 0.1 µM both on IK1 and on the action potential duration (APD). Experiments were performed by the whole-cell patch-clamp technique on enzymatically isolated rat ventricular cardiomyocytes, mostly at 23°C with the exception of APD measurements which were performed at 37°C as well. Sildenafil caused a significant, reversible, and concentration-dependent inhibition of IK1 that did not differ at −50 and −110 mV. Simultaneous application of sildenafil and Ba2+ at 0.1 µM revealed a massive inhibition of both inward and outward components of IK1 (this synergy was missing at other tested combinations). The combined effect at 0.1 µM (45.7 ± 5.7 and 43.0 ± 6.9% inhibition at −50 and −110 mV, respectively) was significantly higher than a simple sum of almost negligible effects of the individual substances and it led to a significant prolongation of APD at both 23 and 37°C. To our knowledge, similar potentiation of the drug-channel interaction has not been described. The observed massive inhibition of IK1 induced by a combined action of the vasodilator sildenafil and environmental contaminant Ba2+ at a low concentration and resulting in a significant APD prolongation may contribute to the genesis of arrhythmias observed in some patients treated with sildenafil.
ER  -

MACHÁČEK, Martin, Olga ŠVECOVÁ a Markéta BÉBAROVÁ. Combination of Sildenafil and Ba2+ at a Low Concentration Show a Significant Synergistic Inhibition of Inward Rectifier Potassium Current Resulting in Action Potential Prolongation. \textit{Frontiers in Pharmacology}. LAUSANNE: FRONTIERS MEDIA S, 2022, roč.~13, April 2022, s.~1-9. ISSN~1663-9812. Dostupné z: https://dx.doi.org/10.3389/fphar.2022.829952.

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