EXPRESSION OF GENES RESPONSIBLE FOR CELL MORPHOGENESIS INVOLVED
IN DIFFERENTIATION IN PORCINE BUCCAL POUCH MUCOSAL CELLS DURING
LONG-TERM PRIMARY CULTURE AND REAL-TIME PROLIFERATION IN VITRO

J 2017

EXPRESSION OF GENES RESPONSIBLE FOR CELL MORPHOGENESIS INVOLVED IN DIFFERENTIATION IN PORCINE BUCCAL POUCH MUCOSAL CELLS DURING LONG-TERM PRIMARY CULTURE AND REAL-TIME PROLIFERATION IN VITRO

DYSZKIEWICZ-KONWINSKA, M., A. BRYJA, K. JOPEK, J. BUDNA, R. KHOZMI et. al.

Základní údaje

Originální název

EXPRESSION OF GENES RESPONSIBLE FOR CELL MORPHOGENESIS INVOLVED IN DIFFERENTIATION IN PORCINE BUCCAL POUCH MUCOSAL CELLS DURING LONG-TERM PRIMARY CULTURE AND REAL-TIME PROLIFERATION IN VITRO

Autoři

DYSZKIEWICZ-KONWINSKA, M., A. BRYJA, K. JOPEK, J. BUDNA, R. KHOZMI, Michal JEŠETA (203 Česká republika, domácí), D. BUKOWSKA, P. ANTOSIK, M. BRUSKA, M. NOWICKI, M. ZABEL a B. KEMPISTY (garant)

Vydání

Journal of biological regulators and homeostatic agents, Silva Marina, Biolife SAS, 2017, 0393-974X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.397

Kód RIV

RIV/00216224:14110/17:00124636

Organizační jednotka

Lékařská fakulta

UT WoS

000426184600003

Klíčová slova anglicky

mucosal cells; pig; primary culture

Štítky

14110411, rivok

Změněno: 20. 5. 2022 09:27, Mgr. Tereza Miškechová

Anotace

V originále

Recently, using experimental animal model, we demonstrated that porcine buccal pouch mucosal cells reflect increased proliferation capability during primary cultivation in vitro. Although the histological structure and morphogenesis in oral cavity is well recognized, the molecular mechanisms which regulate this process still need further investigation. This study was aimed to analyze the molecular marker expression profile involved in morphogenesis and differentiation capacity of porcine buccal pouch mucosal cells during their long-term primary cultivation in vitro. The experiment was performed on buccal pouch mucosal cells isolated from 80 pubertal crossbred Landrace gilts. After collection, the cells were treated enzymatically and transferred into a primary in vitro culture (IVC) system and cultured for 30 days. The cells were collected for RNA isolation after 7, 15 and 30 days of IVC and were checked for their real-time proliferative status using the RTCA system. We found an increased expression of FN1 and SOX9 genes when calculated against ACTB after 7, and 30 days of IVC, (P less than 0.01, P less than 0.001, respectively). The CXCL12 mRNA was down-regulated after 7, 15 and 30 days of IVC, but not statistically significant. Similar expression profile was observed when calculated against HPRT, however, DAB2 was found to be higher expressed at day 15 of IVC, (P less than 0.05). The cell index measured during real-time cell proliferation was substantially increased between 96 h and 147h of IVC and reached the log phase. Since FN1 and SOX9 revealed significant increase of expression after long-term culture in vitro, it is suggested that expression of these differentiation and stemness genes is accompanied by cell proliferation. Moreover, FN1 and SOX9 might be recognized as new markers of buccal pouch mucosal cell proliferation and differentiation in pigs in in vitro primary culture model.

Citovat

DYSZKIEWICZ-KONWINSKA, M., A. BRYJA, K. JOPEK, J. BUDNA, R. KHOZMI, Michal JEŠETA, D. BUKOWSKA, P. ANTOSIK, M. BRUSKA, M. NOWICKI, M. ZABEL a B. KEMPISTY. EXPRESSION OF GENES RESPONSIBLE FOR CELL MORPHOGENESIS INVOLVED IN DIFFERENTIATION IN PORCINE BUCCAL POUCH MUCOSAL CELLS DURING LONG-TERM PRIMARY CULTURE AND REAL-TIME PROLIFERATION IN VITRO. Journal of biological regulators and homeostatic agents. Silva Marina: Biolife SAS, 2017, roč. 31, č. 4, s. 855-864. ISSN 0393-974X.

@article{1854515,
   author = {DyszkiewiczandKonwinska, M. and Bryja, A. and Jopek, K. and Budna, J. and Khozmi, R. and Ješeta, Michal and Bukowska, D. and Antosik, P. and Bruska, M. and Nowicki, M. and Zabel, M. and Kempisty, B.},
   article_location = {Silva Marina},
   article_number = {4},
   keywords = {mucosal cells; pig; primary culture},
   language = {eng},
   issn = {0393-974X},
   journal = {Journal of biological regulators and homeostatic agents},
   title = {EXPRESSION OF GENES RESPONSIBLE FOR CELL MORPHOGENESIS INVOLVED IN DIFFERENTIATION IN PORCINE BUCCAL POUCH MUCOSAL CELLS DURING LONG-TERM PRIMARY CULTURE AND REAL-TIME PROLIFERATION IN VITRO},
   url = {https://pubmed.ncbi.nlm.nih.gov/29254288/},
   volume = {31},
   year = {2017}
}

TY  - JOUR
ID  - 1854515
AU  - Dyszkiewicz-Konwinska, M. - Bryja, A. - Jopek, K. - Budna, J. - Khozmi, R. - Ješeta, Michal - Bukowska, D. - Antosik, P. - Bruska, M. - Nowicki, M. - Zabel, M. - Kempisty, B.
PY  - 2017
TI  - EXPRESSION OF GENES RESPONSIBLE FOR CELL MORPHOGENESIS INVOLVED IN DIFFERENTIATION IN PORCINE BUCCAL POUCH MUCOSAL CELLS DURING LONG-TERM PRIMARY CULTURE AND REAL-TIME PROLIFERATION IN VITRO
JF  - Journal of biological regulators and homeostatic agents
VL  - 31
IS  - 4
SP  - 855-864
EP  - 855-864
PB  - Biolife SAS
SN  - 0393974X
KW  - mucosal cells
KW  - pig
KW  - primary culture
UR  - https://pubmed.ncbi.nlm.nih.gov/29254288/
N2  - Recently, using experimental animal model, we demonstrated that porcine buccal pouch mucosal cells reflect increased proliferation capability during primary cultivation in vitro. Although the histological structure and morphogenesis in oral cavity is well recognized, the molecular mechanisms which regulate this process still need further investigation. This study was aimed to analyze the molecular marker expression profile involved in morphogenesis and differentiation capacity of porcine buccal pouch mucosal cells during their long-term primary cultivation in vitro. The experiment was performed on buccal pouch mucosal cells isolated from 80 pubertal crossbred Landrace gilts. After collection, the cells were treated enzymatically and transferred into a primary in vitro culture (IVC) system and cultured for 30 days. The cells were collected for RNA isolation after 7, 15 and 30 days of IVC and were checked for their real-time proliferative status using the RTCA system. We found an increased expression of FN1 and SOX9 genes when calculated against ACTB after 7, and 30 days of IVC, (P less than 0.01, P less than 0.001, respectively). The CXCL12 mRNA was down-regulated after 7, 15 and 30 days of IVC, but not statistically significant. Similar expression profile was observed when calculated against HPRT, however, DAB2 was found to be higher expressed at day 15 of IVC, (P less than 0.05). The cell index measured during real-time cell proliferation was substantially increased between 96 h and 147h of IVC and reached the log phase. Since FN1 and SOX9 revealed significant increase of expression after long-term culture in vitro, it is suggested that expression of these differentiation and stemness genes is accompanied by cell proliferation. Moreover, FN1 and SOX9 might be recognized as new markers of buccal pouch mucosal cell proliferation and differentiation in pigs in in vitro primary culture model.
ER  -

DYSZKIEWICZ-KONWINSKA, M., A. BRYJA, K. JOPEK, J. BUDNA, R. KHOZMI, Michal JEŠETA, D. BUKOWSKA, P. ANTOSIK, M. BRUSKA, M. NOWICKI, M. ZABEL a B. KEMPISTY. EXPRESSION OF GENES RESPONSIBLE FOR CELL MORPHOGENESIS INVOLVED IN DIFFERENTIATION IN PORCINE BUCCAL POUCH MUCOSAL CELLS DURING LONG-TERM PRIMARY CULTURE AND REAL-TIME PROLIFERATION IN VITRO. \textit{Journal of biological regulators and homeostatic agents}. Silva Marina: Biolife SAS, 2017, roč.~31, č.~4, s.~855-864. ISSN~0393-974X.

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