Bioactive molecules produced by Heterorhabditis bacteriophora
affects the phenoloxidase system of Galleria mellonella

a 2022

Bioactive molecules produced by Heterorhabditis bacteriophora affects the phenoloxidase system of Galleria mellonella

ELIÁŠ, Sara, Jana HURYCHOVÁ, Pavel DOBEŠ, Duarte Nuno TOUBARRO TIAGO, Nelson SIMÕES et. al.

Základní údaje

Originální název

Bioactive molecules produced by Heterorhabditis bacteriophora affects the phenoloxidase system of Galleria mellonella

Autoři

ELIÁŠ, Sara (276 Německo, garant, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Duarte Nuno TOUBARRO TIAGO (620 Portugalsko), Nelson SIMÕES (620 Portugalsko) a Pavel HYRŠL (203 Česká republika, domácí)

Vydání

Seventh International Congress of Nematology, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10602 Biology , Evolutionary biology

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/22:00129439

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Heterorhabditis bacteriophora; phenoloxidase; Galleria mellonella; excreted/secreted products

Změněno: 1. 6. 2022 16:28, doc. RNDr. Pavel Hyršl, Ph.D.

Anotace

V originále

Entomopathogenic nematodes are able to produce bioactive molecules referred as excreted/secreted products (ESPs). The ESPs are small molecules, proteins and nucleic acids which can interfere with the host immune system in order to increase the chance of entomopathogenic nematodes reproduction. We tested the effect of ESPs obtained from Heterorhabditis bacteriophora on immune system of Galleria mellonella larvae. The results indicated suppression of phenoloxidase activity after administration of isolated ESPs. Subsequently, the isolated ESPs were purified in two steps to obtain and identify the active fraction. Firstly, the ESPs were purified based on their charge resulting in five distinct fractions. Two of them inhibited phenoloxidase activity of G. mellonella larvae. These active fractions were subjected to second purification step in which ESPs were separated based on their molecular weight. We obtained seven fractions and we tested their effect on phenoloxidase activity. The purified molecules in fraction with molecular mass of 38 kDa significantly inhibited phenoloxidase activity of G. mellonella larvae. Oncoming analysis of purified active molecules are focused on their identification by mass spectroscopy. The immunosuppressive nature of ESPs is possible to utilize as the biological control of insect pests in agriculture. It is of note that some ESPs contain also compounds with high bioactivity which are able to affect the mammalian immunity or act as the antibiotic agent. Because of these properties, the ESPs can be used as an interesting source of bioactive molecules with potential use in pharmacology. This study was supported by grant from The Ministry of Agriculture of the Czech Republic (QK1910286).

Návaznosti

QK1910286, projekt VaV

Název: Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

Investor: Ministerstvo zemědělství ČR, Efektivní postupy a strategie pro zvládání včelích chorob a udržitelný chov včelstev

Citovat

ELIÁŠ, Sara, Jana HURYCHOVÁ, Pavel DOBEŠ, Duarte Nuno TOUBARRO TIAGO, Nelson SIMÕES a Pavel HYRŠL. Bioactive molecules produced by Heterorhabditis bacteriophora affects the phenoloxidase system of Galleria mellonella. In Seventh International Congress of Nematology. 2022.

@proceedings{1857240,
   author = {Eliáš, Sara and Hurychová, Jana and Dobeš, Pavel and Toubarro Tiago, Duarte Nuno and Simões, Nelson and Hyršl, Pavel},
   booktitle = {Seventh International Congress of Nematology},
   keywords = {Heterorhabditis bacteriophora; phenoloxidase; Galleria mellonella; excreted/secreted products},
   language = {eng},
   title = {Bioactive molecules produced by Heterorhabditis bacteriophora affects the phenoloxidase system of Galleria mellonella},
   year = {2022}
}

TY  - CONF
ID  - 1857240
AU  - Eliáš, Sara - Hurychová, Jana - Dobeš, Pavel - Toubarro Tiago, Duarte Nuno - Simões, Nelson - Hyršl, Pavel
PY  - 2022
TI  - Bioactive molecules produced by Heterorhabditis bacteriophora affects the phenoloxidase system of Galleria mellonella
KW  - Heterorhabditis bacteriophora
KW  - phenoloxidase
KW  - Galleria mellonella
KW  - excreted/secreted products
N2  - Entomopathogenic nematodes are able to produce bioactive molecules referred as excreted/secreted products (ESPs). The ESPs are small molecules, proteins and nucleic acids which can interfere with the host immune system in order to increase the chance of entomopathogenic nematodes reproduction. We tested the effect of ESPs obtained from Heterorhabditis bacteriophora on immune system of Galleria mellonella larvae. The results indicated suppression of phenoloxidase activity after administration of isolated ESPs. Subsequently, the isolated ESPs were purified in two steps to obtain and identify the active fraction. Firstly, the ESPs were purified based on their charge resulting in five distinct fractions. Two of them inhibited phenoloxidase activity of G. mellonella larvae. These active fractions were subjected to second purification step in which ESPs were separated based on their molecular weight. We obtained seven fractions and we tested their effect on phenoloxidase activity. The purified molecules in fraction with molecular mass of 38 kDa significantly inhibited phenoloxidase activity of G. mellonella larvae. Oncoming analysis of purified active molecules are focused on their identification by mass spectroscopy. The immunosuppressive nature of ESPs is possible to utilize as the biological control of insect pests in agriculture. It is of note that some ESPs contain also compounds with high bioactivity which are able to affect the mammalian immunity or act as the antibiotic agent. Because of these properties, the ESPs can be used as an interesting source of bioactive molecules with potential use in pharmacology. This study was supported by grant from The Ministry of Agriculture of the Czech Republic (QK1910286).
ER  -

ELIÁŠ, Sara, Jana HURYCHOVÁ, Pavel DOBEŠ, Duarte Nuno TOUBARRO TIAGO, Nelson SIMÕES a Pavel HYRŠL. Bioactive molecules produced by Heterorhabditis bacteriophora affects the phenoloxidase system of Galleria mellonella. In \textit{Seventh International Congress of Nematology}. 2022.

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