Proteases of Schistosoma mansoni and Fasciola hepatica eggs

a 2021

Proteases of Schistosoma mansoni and Fasciola hepatica eggs

PETERKOVÁ, Kristýna, Jiří VOREL, Jana ILGOVÁ, Lucie ŠKORPÍKOVÁ, Pavel OSTAŠOV et. al.

Základní údaje

Originální název

Proteases of Schistosoma mansoni and Fasciola hepatica eggs

Autoři

PETERKOVÁ, Kristýna, Jiří VOREL, Jana ILGOVÁ, Lucie ŠKORPÍKOVÁ, Pavel OSTAŠOV, Lukáš KONEČNÝ, Martin KAŠNÝ, Martin HORN a Jan DVOŘÁK

Vydání

26th Helminthological Days, 2021

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-906644-9-4

Klíčová slova anglicky

Proteases, protease inhibitors, Fasciola hepatica, Schistosoma mansoni, eggs, immun response

Změněno: 16. 6. 2022 12:29, Mgr. Lucie Škorpíková, Ph.D.

Anotace

V originále

Eggs of blood fluke Schistosoma mansoni are the main causative agent of clinical manifestations of a disease called schistosomiasis. In the definitive host, eggs are laid in the mesenteric veins and then migrate through the intestinal wall to the gut and outer environment. A large proportion of eggs, however, become entrapped in host tissues, typically the liver, causing inflammation and tissue damage. During this process, eggs secrete molecules to modulate host immune response. We hypothesize that proteases and protease inhibitors play an important role during the infection. Our focus in this project is to identify them and measure their transcriptional levels in undeveloped and developed eggs. Additionally, we included a comparison with eggs of another trematode Fasciola hepatica. These eggs pass from host bile ducts to the gut lumen and outer environment without any known pathological consequences for the host and without penetrating the tissues. Therefore, we employ this organism as a useful comparative subject to decipher which proteases and inhibitors are common and which are unique for each of them. We isolated RNA from several developmental stages of eggs of both organisms, prepared cDNA libraries, performed Illumina sequencing and analyzed transcripts using a pipeline of bioinformatics tools. By synthesis of database identifications and manual curation, we obtained a dataset of expressed proteases and protease inhibitors. Next, we identified orthologous proteins for both species and compared expression levels between them.

Návaznosti

GA19-17269S, projekt VaV

Název: Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace (Akronym: Proteolýza vajec červů)

Investor: Grantová agentura ČR, Proteolýza ve vejcích parazitických červů: úloha v patologii a její regulace

Citovat

PETERKOVÁ, Kristýna, Jiří VOREL, Jana ILGOVÁ, Lucie ŠKORPÍKOVÁ, Pavel OSTAŠOV, Lukáš KONEČNÝ, Martin KAŠNÝ, Martin HORN a Jan DVOŘÁK. Proteases of Schistosoma mansoni and Fasciola hepatica eggs. In 26th Helminthological Days. 2021. ISBN 978-80-906644-9-4.

@proceedings{1860577,
   author = {Peterková, Kristýna and Vorel, Jiří and Ilgová, Jana and Škorpíková, Lucie and Ostašov, Pavel and Konečný, Lukáš and Kašný, Martin and Horn, Martin and Dvořák, Jan},
   booktitle = {26th Helminthological Days},
   keywords = {Proteases, protease inhibitors, Fasciola hepatica, Schistosoma mansoni, eggs, immun response},
   language = {eng},
   isbn = {978-80-906644-9-4},
   title = {Proteases of Schistosoma mansoni and Fasciola hepatica eggs},
   year = {2021}
}

TY  - CONF
ID  - 1860577
AU  - Peterková, Kristýna - Vorel, Jiří - Ilgová, Jana - Škorpíková, Lucie - Ostašov, Pavel - Konečný, Lukáš - Kašný, Martin - Horn, Martin - Dvořák, Jan
PY  - 2021
TI  - Proteases of Schistosoma mansoni and Fasciola hepatica eggs
SN  - 9788090664494
KW  - Proteases, protease inhibitors, Fasciola hepatica, Schistosoma mansoni, eggs, immun response
N2  - Eggs of blood fluke Schistosoma mansoni are the main causative agent of clinical manifestations of a disease called schistosomiasis. In the definitive host, eggs are laid in the mesenteric veins and then migrate through the intestinal wall to the gut and outer environment. A large proportion of eggs, however, become entrapped in host tissues, typically the liver, causing inflammation and tissue damage. During this process, eggs secrete molecules to modulate host immune response. We hypothesize that proteases and protease inhibitors play an important role during the infection. Our focus in this project is to identify them and measure their transcriptional levels in undeveloped and developed eggs. Additionally, we included a comparison with eggs of another trematode Fasciola hepatica. These eggs pass from host bile ducts to the gut lumen and outer environment without any known pathological consequences for the host and without penetrating the tissues. Therefore, we employ this organism as a useful comparative subject to decipher which proteases and inhibitors are common and which are unique for each of them. We isolated RNA from several developmental stages of eggs of both organisms, prepared cDNA libraries, performed Illumina sequencing and analyzed transcripts using a pipeline of bioinformatics tools. By synthesis of database identifications and manual curation, we obtained a dataset of expressed proteases and protease inhibitors. Next, we identified orthologous proteins for both species and compared expression levels between them.
ER  -

PETERKOVÁ, Kristýna, Jiří VOREL, Jana ILGOVÁ, Lucie ŠKORPÍKOVÁ, Pavel OSTAŠOV, Lukáš KONEČNÝ, Martin KAŠNÝ, Martin HORN a Jan DVOŘÁK. Proteases of Schistosoma mansoni and Fasciola hepatica eggs. In \textit{26th Helminthological Days}. 2021. ISBN~978-80-906644-9-4.

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