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@article{1862251, author = {Folta, Adam and Sasinkova, Markéta and Ďuriníková, Anna and Drncová, Marie and Weinbergerová, Barbora and Mayer, Jiří and Ježíšková, Ivana}, article_location = {DORDRECHT}, article_number = {8}, doi = {http://dx.doi.org/10.1007/s11033-022-07363-8}, keywords = {Nucleophosmine 1; Acute myeloid leukemia; Quantitative PCR standards; Colony selection; Cloning}, language = {eng}, issn = {0301-4851}, journal = {Molecular Biology Reports}, title = {Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia.}, url = {https://link.springer.com/article/10.1007/s11033-022-07363-8}, volume = {49}, year = {2022} }
TY - JOUR ID - 1862251 AU - Folta, Adam - Sasinkova, Markéta - Ďuriníková, Anna - Drncová, Marie - Weinbergerová, Barbora - Mayer, Jiří - Ježíšková, Ivana PY - 2022 TI - Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia. JF - Molecular Biology Reports VL - 49 IS - 8 SP - 8169-8172 EP - 8169-8172 PB - SPRINGER SN - 03014851 KW - Nucleophosmine 1 KW - Acute myeloid leukemia KW - Quantitative PCR standards KW - Colony selection KW - Cloning UR - https://link.springer.com/article/10.1007/s11033-022-07363-8 N2 - Background NPM1 plasmid standards are required for absolute quantification of minimal residual disease in acute myeloid leukemia patients. The standards are usually obtained, next to commercially constructed gene fragments, from transgenic bacteria colonies. However, this procedure is laborious and very time consuming. Methods and results We have developed a PCR method that speeds up, simplifies, and streamlines the process of preparing NPM1 plasmid standards. The method is based on a combination of three primers, two surrounding the usual NPM1 mutation position and one over the mutation site. With this method, we were able to clearly distinguish plasmids with at least 15 different NPM1 mutations from the wild-type NPM1 plasmid. Conclusions With the new approach, preparing NPM1 plasmid standards is easier, identifying NPM1-positive colonies is possible in less than a day and moreover, for a lower price than commercially constructed gene fragments. ER -
FOLTA, Adam, Markéta SASINKOVA, Anna ĎURINÍKOVÁ, Marie DRNCOVÁ, Barbora WEINBERGEROVÁ, Jiří MAYER a Ivana JEŽÍŠKOVÁ. Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia. \textit{Molecular Biology Reports}. DORDRECHT: SPRINGER, 2022, roč.~49, č.~8, s.~8169-8172. ISSN~0301-4851. Dostupné z: https://dx.doi.org/10.1007/s11033-022-07363-8.
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