Effective NPM1 plasmid standards selection for
minimal/measurable residual disease monitoring in acute myeloid
leukemia.

J 2022

Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia.

FOLTA, Adam, Markéta SASINKOVA, Anna ĎURINÍKOVÁ, Marie DRNCOVÁ, Barbora WEINBERGEROVÁ et. al.

Základní údaje

Originální název

Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia.

Autoři

FOLTA, Adam (203 Česká republika), Markéta SASINKOVA (203 Česká republika), Anna ĎURINÍKOVÁ (703 Slovensko, domácí), Marie DRNCOVÁ (203 Česká republika, domácí), Barbora WEINBERGEROVÁ (203 Česká republika, domácí), Jiří MAYER (203 Česká republika, domácí) a Ivana JEŽÍŠKOVÁ (203 Česká republika, garant, domácí)

Vydání

Molecular Biology Reports, DORDRECHT, SPRINGER, 2022, 0301-4851

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30205 Hematology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.800

Kód RIV

RIV/00216224:14110/22:00126108

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s11033-022-07363-8

UT WoS

000812606600010

Klíčová slova anglicky

Nucleophosmine 1; Acute myeloid leukemia; Quantitative PCR standards; Colony selection; Cloning

Štítky

14110212, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 12. 2022 12:37, Mgr. Tereza Miškechová

Anotace

V originále

Background NPM1 plasmid standards are required for absolute quantification of minimal residual disease in acute myeloid leukemia patients. The standards are usually obtained, next to commercially constructed gene fragments, from transgenic bacteria colonies. However, this procedure is laborious and very time consuming. Methods and results We have developed a PCR method that speeds up, simplifies, and streamlines the process of preparing NPM1 plasmid standards. The method is based on a combination of three primers, two surrounding the usual NPM1 mutation position and one over the mutation site. With this method, we were able to clearly distinguish plasmids with at least 15 different NPM1 mutations from the wild-type NPM1 plasmid. Conclusions With the new approach, preparing NPM1 plasmid standards is easier, identifying NPM1-positive colonies is possible in less than a day and moreover, for a lower price than commercially constructed gene fragments.

Návaznosti

MUNI/A/1330/2021, interní kód MU

Název: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX (Akronym: VýDiTeHeMa IX)

Investor: Masarykova univerzita, Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX

Citovat

FOLTA, Adam, Markéta SASINKOVA, Anna ĎURINÍKOVÁ, Marie DRNCOVÁ, Barbora WEINBERGEROVÁ, Jiří MAYER a Ivana JEŽÍŠKOVÁ. Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia. Molecular Biology Reports. DORDRECHT: SPRINGER, 2022, roč. 49, č. 8, s. 8169-8172. ISSN 0301-4851. Dostupné z: https://dx.doi.org/10.1007/s11033-022-07363-8.

@article{1862251,
   author = {Folta, Adam and Sasinkova, Markéta and Ďuriníková, Anna and Drncová, Marie and Weinbergerová, Barbora and Mayer, Jiří and Ježíšková, Ivana},
   article_location = {DORDRECHT},
   article_number = {8},
   doi = {http://dx.doi.org/10.1007/s11033-022-07363-8},
   keywords = {Nucleophosmine 1; Acute myeloid leukemia; Quantitative PCR standards; Colony selection; Cloning},
   language = {eng},
   issn = {0301-4851},
   journal = {Molecular Biology Reports},
   title = {Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia.},
   url = {https://link.springer.com/article/10.1007/s11033-022-07363-8},
   volume = {49},
   year = {2022}
}

TY  - JOUR
ID  - 1862251
AU  - Folta, Adam - Sasinkova, Markéta - Ďuriníková, Anna - Drncová, Marie - Weinbergerová, Barbora - Mayer, Jiří - Ježíšková, Ivana
PY  - 2022
TI  - Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia.
JF  - Molecular Biology Reports
VL  - 49
IS  - 8
SP  - 8169-8172
EP  - 8169-8172
PB  - SPRINGER
SN  - 03014851
KW  - Nucleophosmine 1
KW  - Acute myeloid leukemia
KW  - Quantitative PCR standards
KW  - Colony selection
KW  - Cloning
UR  - https://link.springer.com/article/10.1007/s11033-022-07363-8
N2  - Background NPM1 plasmid standards are required for absolute quantification of minimal residual disease in acute myeloid leukemia patients. The standards are usually obtained, next to commercially constructed gene fragments, from transgenic bacteria colonies. However, this procedure is laborious and very time consuming. Methods and results We have developed a PCR method that speeds up, simplifies, and streamlines the process of preparing NPM1 plasmid standards. The method is based on a combination of three primers, two surrounding the usual NPM1 mutation position and one over the mutation site. With this method, we were able to clearly distinguish plasmids with at least 15 different NPM1 mutations from the wild-type NPM1 plasmid. Conclusions With the new approach, preparing NPM1 plasmid standards is easier, identifying NPM1-positive colonies is possible in less than a day and moreover, for a lower price than commercially constructed gene fragments.
ER  -

FOLTA, Adam, Markéta SASINKOVA, Anna ĎURINÍKOVÁ, Marie DRNCOVÁ, Barbora WEINBERGEROVÁ, Jiří MAYER a Ivana JEŽÍŠKOVÁ. Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia. \textit{Molecular Biology Reports}. DORDRECHT: SPRINGER, 2022, roč.~49, č.~8, s.~8169-8172. ISSN~0301-4851. Dostupné z: https://dx.doi.org/10.1007/s11033-022-07363-8.

Podrobný výpis o publikaci