Potential estrogenic background in aquatic laboratory
cultivations

J 2022

Potential estrogenic background in aquatic laboratory cultivations

SYCHROVÁ, Eliška, Marie SMUTNÁ, Kateřina NOVÁKOVÁ a Klára HILSCHEROVÁ

Základní údaje

Originální název

Potential estrogenic background in aquatic laboratory cultivations

Autoři

SYCHROVÁ, Eliška (203 Česká republika, domácí), Marie SMUTNÁ (203 Česká republika, domácí), Kateřina NOVÁKOVÁ (203 Česká republika, domácí) a Klára HILSCHEROVÁ (203 Česká republika, garant, domácí)

Vydání

Aquatic toxicology, AMSTERDAM, ELSEVIER SCIENCE BV, 2022, 0166-445X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10617 Marine biology, freshwater biology, limnology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.500

Kód RIV

RIV/00216224:14310/22:00126176

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.aquatox.2022.106169

UT WoS

000796190900002

Klíčová slova anglicky

Estrogenicity; Aquatic medium; Alkylphenols; Bisphenol A; Aeration

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 6. 2022 22:03, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Aquatic biotests are important tools targeting various effects in ecotoxicology, including endocrine disruption. Unintentional exposure of bioassay organisms to endocrine disruptors during cultivation or testing may interfere with assessed endpoints. We illustrate this issue on the example of laboratory phytoplankton cultivation, where possible sources of estrogenic compounds have been revealed. Fifty-four blank samples (water and fresh or cultivated growth media) were assessed by in vitro biotests for their estrogenicity, and major known estrogens originating from plastic materials, bisphenol A and alkylphenols, were analyzed in selected samples. The samples of freshly prepared growth medium elicited weak estrogenic response in bioassays and some samples of the aerated media caused responses even above the 50% of maximum of the reference compound (17??-estradiol, E2), while the samples from diverse laboratory water sources did not show significant estrogenic activity. The results identified substances contained in the growth medium as minor but reproducible contributors to estrogenicity in the cultivations. Sporadic but significant effects (up to 4.9 ng E2 equivalent/L) can be ascribed to compounds released from the used plastic materials during aeration of the cultivations. The potential sources of uninten-tional exposure to estrogenic compounds need to be considered in aquatic cultivations and biotests, since they could impact their outcomes, especially in arrangements assessing reproduction or whole life cycle biotests, or production of bioactive compounds by phytoplankton. The findings emphasize the necessity to assess all relevant blanks, ideally by sensitive high throughput in vitro assays that reflect also unknown pollutants and minimize all potential sources of background contamination. In vitro assays show very good applicability for this purpose since they enable to screen for any background estrogenicity of the used media and materials without the need of analyzing individual compounds, which often might not be known.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

GX20-04676X, projekt VaV

Název: Holistická charakterizace expozice a potenciálních účinků komplexních směsí chemických látek ve vodním prostředí

Investor: Grantová agentura ČR, Holistická charakterizace expozice a potenciálních účinků komplexních směsí chemických látek ve vodním prostředí

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

Citovat

SYCHROVÁ, Eliška, Marie SMUTNÁ, Kateřina NOVÁKOVÁ a Klára HILSCHEROVÁ. Potential estrogenic background in aquatic laboratory cultivations. Aquatic toxicology. AMSTERDAM: ELSEVIER SCIENCE BV, 2022, roč. 247, June 2022, s. 1-4. ISSN 0166-445X. Dostupné z: https://dx.doi.org/10.1016/j.aquatox.2022.106169.

@article{1863608,
   author = {Sychrová, Eliška and Smutná, Marie and Nováková, Kateřina and Hilscherová, Klára},
   article_location = {AMSTERDAM},
   article_number = {June 2022},
   doi = {http://dx.doi.org/10.1016/j.aquatox.2022.106169},
   keywords = {Estrogenicity; Aquatic medium; Alkylphenols; Bisphenol A; Aeration},
   language = {eng},
   issn = {0166-445X},
   journal = {Aquatic toxicology},
   title = {Potential estrogenic background in aquatic laboratory cultivations},
   url = {https://www.sciencedirect.com/science/article/pii/S0166445X22000959?via%3Dihub},
   volume = {247},
   year = {2022}
}

TY  - JOUR
ID  - 1863608
AU  - Sychrová, Eliška - Smutná, Marie - Nováková, Kateřina - Hilscherová, Klára
PY  - 2022
TI  - Potential estrogenic background in aquatic laboratory cultivations
JF  - Aquatic toxicology
VL  - 247
IS  - June 2022
SP  - 1-4
EP  - 1-4
PB  - ELSEVIER SCIENCE BV
SN  - 0166445X
KW  - Estrogenicity
KW  - Aquatic medium
KW  - Alkylphenols
KW  - Bisphenol A
KW  - Aeration
UR  - https://www.sciencedirect.com/science/article/pii/S0166445X22000959?via%3Dihub
N2  - Aquatic biotests are important tools targeting various effects in ecotoxicology, including endocrine disruption. Unintentional exposure of bioassay organisms to endocrine disruptors during cultivation or testing may interfere with assessed endpoints. We illustrate this issue on the example of laboratory phytoplankton cultivation, where possible sources of estrogenic compounds have been revealed. Fifty-four blank samples (water and fresh or cultivated growth media) were assessed by in vitro biotests for their estrogenicity, and major known estrogens originating from plastic materials, bisphenol A and alkylphenols, were analyzed in selected samples. The samples of freshly prepared growth medium elicited weak estrogenic response in bioassays and some samples of the aerated media caused responses even above the 50% of maximum of the reference compound (17??-estradiol, E2), while the samples from diverse laboratory water sources did not show significant estrogenic activity. The results identified substances contained in the growth medium as minor but reproducible contributors to estrogenicity in the cultivations. Sporadic but significant effects (up to 4.9 ng E2 equivalent/L) can be ascribed to compounds released from the used plastic materials during aeration of the cultivations. The potential sources of uninten-tional exposure to estrogenic compounds need to be considered in aquatic cultivations and biotests, since they could impact their outcomes, especially in arrangements assessing reproduction or whole life cycle biotests, or production of bioactive compounds by phytoplankton. The findings emphasize the necessity to assess all relevant blanks, ideally by sensitive high throughput in vitro assays that reflect also unknown pollutants and minimize all potential sources of background contamination. In vitro assays show very good applicability for this purpose since they enable to screen for any background estrogenicity of the used media and materials without the need of analyzing individual compounds, which often might not be known.
ER  -

SYCHROVÁ, Eliška, Marie SMUTNÁ, Kateřina NOVÁKOVÁ a Klára HILSCHEROVÁ. Potential estrogenic background in aquatic laboratory cultivations. \textit{Aquatic toxicology}. AMSTERDAM: ELSEVIER SCIENCE BV, 2022, roč.~247, June 2022, s.~1-4. ISSN~0166-445X. Dostupné z: https://dx.doi.org/10.1016/j.aquatox.2022.106169.

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