2022
Bioactive molecules produced by Heterorhabditis bacteriophora after in vitro stimulation by insect tissue
ELIÁŠ, Sara, Jana ILGOVÁ, Jana HURYCHOVÁ, Paola ALBERT POYÁN, Pavel DOBEŠ et. al.Základní údaje
Originální název
Bioactive molecules produced by Heterorhabditis bacteriophora after in vitro stimulation by insect tissue
Název česky
Bioactive molecules produced by Heterorhabditis bacteriophora after in vitro stimulation by insect tissue
Autoři
ELIÁŠ, Sara (276 Německo, domácí), Jana ILGOVÁ (703 Slovensko, domácí), Jana HURYCHOVÁ (203 Česká republika, domácí), Paola ALBERT POYÁN (724 Španělsko, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Martin KAŠNÝ (203 Česká republika) a Pavel HYRŠL (203 Česká republika, garant, domácí)
Vydání
18th meeting IOBC/WPRS Working Group Microbial and Nematode Control of Invertebrate Pests, 2022
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10616 Entomology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Kód RIV
RIV/00216224:14310/22:00129440
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
Heterorhabditis bacteriophora; secreted/excreted products; entomopathogenic nematodes
Klíčová slova anglicky
Heterorhabditis bacteriophora; secreted/excreted products; entomopathogenic nematodes
Příznaky
Mezinárodní význam
Změněno: 12. 7. 2022 15:48, Mgr. Pavel Dobeš, Ph.D.
V originále
Entomopathogenic nematodes produce bioactive molecules referred to as excreted/secreted products (ESPs). The ESPs comprise a mixture of proteins, lipids, glycans, and nucleic acids that can interfere with the host immune system in order to increase the chance of entomopathogenic nematodes to reproduce. Infective juveniles (IJs), the only free-living stage of nematodes, release ESPs typically in the early phase of infection when contacted with host tissues. The spectrum of ESPs has not yet been fully described, however, its quantitative and qualitative changes during the process of nematode infection were observed. Of particular importance are the immune-modulating proteins, such as proteolytic enzymes and their inhibitors, which have not yet been largely investigated. There are several approaches how to in vitro stimulate IJs to produce ESPs. We investigated the possible effect of various induction materials and time-related parameters on the yield of ESPs. We induced IJs of Heterorhabditis bacteriophora using four differently prepared Galleria mellonella homogenates and tested two time points of IJs induction and subsequent ESPs production. Based on our findings, the time of the IJs induction does not significantly affect the protein concentration of collected ESPs. However, we observed that the induction material affects the ESPs protein quantity. The collected ESPs were further characterised by mass spectrometry to identify proteins present in ESPs and suggest their function. According to GO annotation of mass spectrometric data, lipid-binding proteins, peptidase inhibitors, peptidases, and proteins with chitinase activity are among the most abundant groups of ESP components. As one of the major fractions determined by mass spectrometry were peptidases, we further focussed on their proteolytic activity and observed the presence of serine proteases. Our preliminary mass spectrometry data allow us to continue with identification and functional characterization of candidate bioactive molecules.
Česky
Entomopathogenic nematodes produce bioactive molecules referred to as excreted/secreted products (ESPs). The ESPs comprise a mixture of proteins, lipids, glycans, and nucleic acids that can interfere with the host immune system in order to increase the chance of entomopathogenic nematodes to reproduce. Infective juveniles (IJs), the only free-living stage of nematodes, release ESPs typically in the early phase of infection when contacted with host tissues. The spectrum of ESPs has not yet been fully described, however, its quantitative and qualitative changes during the process of nematode infection were observed. Of particular importance are the immune-modulating proteins, such as proteolytic enzymes and their inhibitors, which have not yet been largely investigated. There are several approaches how to in vitro stimulate IJs to produce ESPs. We investigated the possible effect of various induction materials and time-related parameters on the yield of ESPs. We induced IJs of Heterorhabditis bacteriophora using four differently prepared Galleria mellonella homogenates and tested two time points of IJs induction and subsequent ESPs production. Based on our findings, the time of the IJs induction does not significantly affect the protein concentration of collected ESPs. However, we observed that the induction material affects the ESPs protein quantity. The collected ESPs were further characterised by mass spectrometry to identify proteins present in ESPs and suggest their function. According to GO annotation of mass spectrometric data, lipid-binding proteins, peptidase inhibitors, peptidases, and proteins with chitinase activity are among the most abundant groups of ESP components. As one of the major fractions determined by mass spectrometry were peptidases, we further focussed on their proteolytic activity and observed the presence of serine proteases. Our preliminary mass spectrometry data allow us to continue with identification and functional characterization of candidate bioactive molecules.
Návaznosti
| QK1910286, projekt VaV |
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