Recurrent Plant-Specific Duplications of KNL2 and its Conserved
Function as a Kinetochore Assembly Factor

J 2022

Recurrent Plant-Specific Duplications of KNL2 and its Conserved Function as a Kinetochore Assembly Factor

ZUO, Sheng, Ramakrishna YADALA, Fen YANG, Paul TALBERT, Joerg FUCHS et. al.

Základní údaje

Originální název

Recurrent Plant-Specific Duplications of KNL2 and its Conserved Function as a Kinetochore Assembly Factor

Autoři

ZUO, Sheng (156 Čína, domácí), Ramakrishna YADALA, Fen YANG, Paul TALBERT, Joerg FUCHS, Veit SCHUBERT, Ulkar AHMADLI, Twan RUTTEN, Ales PECINKA, Martin LYSÁK (203 Česká republika, garant, domácí) a Inna LERMONTOVA (276 Německo, domácí)

Vydání

Molecular Biology and Evolution, OXFORD, OXFORD UNIV PRESS, 2022, 0737-4038

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.700

Kód RIV

RIV/00216224:14740/22:00126245

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/molbev/msac123

UT WoS

000819600700002

Klíčová slova anglicky

adaptive evolution; CENH3; centromere; endopolyploidy; gene duplication; kinetochore; KNL2

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2023 13:51, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

KINETOCHORE NULL2 (KNL2) plays key role in the recognition of centromeres and new CENH3 deposition. To gain insight into the origin and diversification of the KNL2 gene, we reconstructed its evolutionary history in the plant kingdom. Our results indicate that the KNL2 gene in plants underwent three independent ancient duplications in ferns, grasses, and eudicots. Additionally, we demonstrated that previously unclassified KNL2 genes could be divided into two clades alpha KNL2 and beta KNL2 in eudicots and gamma KNL2 and delta KNL2 in grasses, respectively. KNL2s of all clades encode the conserved SANTA domain, but only the alpha KNL2 and gamma KNL2 groups additionally encode the CENPC-k motif. In the more numerous eudicot sequences, signatures of positive selection were found in both alpha KNL2 and beta KNL2 clades, suggesting recent or ongoing adaptation. The confirmed centromeric localization of beta KNL2 and mutant analysis suggests that it participates in loading of new CENH3, similarly to alpha KNL2. A high rate of seed abortion was found in heterozygous beta knl2 plants and the germinated homozygous mutants did not develop beyond the seedling stage. Taken together, our study provides a new understanding of the evolutionary diversification of the plant kinetochore assembly gene KNL2, and suggests that the plant-specific duplicated KNL2 genes are involved in centromere and/or kinetochore assembly for preserving genome stability.

Návaznosti

EF17_050/0008496, projekt VaV

Název: MSCAfellow@MUNI

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

Citovat

ZUO, Sheng, Ramakrishna YADALA, Fen YANG, Paul TALBERT, Joerg FUCHS, Veit SCHUBERT, Ulkar AHMADLI, Twan RUTTEN, Ales PECINKA, Martin LYSÁK a Inna LERMONTOVA. Recurrent Plant-Specific Duplications of KNL2 and its Conserved Function as a Kinetochore Assembly Factor. Molecular Biology and Evolution. OXFORD: OXFORD UNIV PRESS, 2022, roč. 39, č. 6, s. nestrankovano, 18 s. ISSN 0737-4038. Dostupné z: https://dx.doi.org/10.1093/molbev/msac123.

@article{1874508,
   author = {Zuo, Sheng and Yadala, Ramakrishna and Yang, Fen and Talbert, Paul and Fuchs, Joerg and Schubert, Veit and Ahmadli, Ulkar and Rutten, Twan and Pecinka, Ales and Lysák, Martin and Lermontova, Inna},
   article_location = {OXFORD},
   article_number = {6},
   doi = {http://dx.doi.org/10.1093/molbev/msac123},
   keywords = {adaptive evolution; CENH3; centromere; endopolyploidy; gene duplication; kinetochore; KNL2},
   language = {eng},
   issn = {0737-4038},
   journal = {Molecular Biology and Evolution},
   title = {Recurrent Plant-Specific Duplications of KNL2 and its Conserved Function as a Kinetochore Assembly Factor},
   url = {https://academic.oup.com/mbe/article/39/6/msac123/6603878?login=true},
   volume = {39},
   year = {2022}
}

TY  - JOUR
ID  - 1874508
AU  - Zuo, Sheng - Yadala, Ramakrishna - Yang, Fen - Talbert, Paul - Fuchs, Joerg - Schubert, Veit - Ahmadli, Ulkar - Rutten, Twan - Pecinka, Ales - Lysák, Martin - Lermontova, Inna
PY  - 2022
TI  - Recurrent Plant-Specific Duplications of KNL2 and its Conserved Function as a Kinetochore Assembly Factor
JF  - Molecular Biology and Evolution
VL  - 39
IS  - 6
SP  - nestrankovano
EP  - nestrankovano
PB  - OXFORD UNIV PRESS
SN  - 07374038
KW  - adaptive evolution
KW  - CENH3
KW  - centromere
KW  - endopolyploidy
KW  - gene duplication
KW  - kinetochore
KW  - KNL2
UR  - https://academic.oup.com/mbe/article/39/6/msac123/6603878?login=true
N2  - KINETOCHORE NULL2 (KNL2) plays key role in the recognition of centromeres and new CENH3 deposition. To gain insight into the origin and diversification of the KNL2 gene, we reconstructed its evolutionary history in the plant kingdom. Our results indicate that the KNL2 gene in plants underwent three independent ancient duplications in ferns, grasses, and eudicots. Additionally, we demonstrated that previously unclassified KNL2 genes could be divided into two clades alpha KNL2 and beta KNL2 in eudicots and gamma KNL2 and delta KNL2 in grasses, respectively. KNL2s of all clades encode the conserved SANTA domain, but only the alpha KNL2 and gamma KNL2 groups additionally encode the CENPC-k motif. In the more numerous eudicot sequences, signatures of positive selection were found in both alpha KNL2 and beta KNL2 clades, suggesting recent or ongoing adaptation. The confirmed centromeric localization of beta KNL2 and mutant analysis suggests that it participates in loading of new CENH3, similarly to alpha KNL2. A high rate of seed abortion was found in heterozygous beta knl2 plants and the germinated homozygous mutants did not develop beyond the seedling stage. Taken together, our study provides a new understanding of the evolutionary diversification of the plant kinetochore assembly gene KNL2, and suggests that the plant-specific duplicated KNL2 genes are involved in centromere and/or kinetochore assembly for preserving genome stability.
ER  -

ZUO, Sheng, Ramakrishna YADALA, Fen YANG, Paul TALBERT, Joerg FUCHS, Veit SCHUBERT, Ulkar AHMADLI, Twan RUTTEN, Ales PECINKA, Martin LYSÁK a Inna LERMONTOVA. Recurrent Plant-Specific Duplications of KNL2 and its Conserved Function as a Kinetochore Assembly Factor. \textit{Molecular Biology and Evolution}. OXFORD: OXFORD UNIV PRESS, 2022, roč.~39, č.~6, s.~nestrankovano, 18 s. ISSN~0737-4038. Dostupné z: https://dx.doi.org/10.1093/molbev/msac123.

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