OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH
THE AIM OF DETECTION OF DANGEROUS VIRUSES

D 2021

OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES

BANÁŠ, Dominik, Ondřej RYCHLÝ, Stefano SALMISTRARO, Ahmet DAVUT AKSU, Miroslava KRZYŽÁNKOVÁ et. al.

Základní údaje

Originální název

OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES

Autoři

BANÁŠ, Dominik (703 Slovensko, domácí), Ondřej RYCHLÝ, Stefano SALMISTRARO, Ahmet DAVUT AKSU (792 Turecko, domácí), Miroslava KRZYŽÁNKOVÁ a René KIZEK (203 Česká republika, domácí)

Vydání

Ostrava, Proceedings 12th International Conference on Nanomaterials - Research & Application, od s. 324-329, 6 s. 2021

Nakladatel

TANGER LTD

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

40301 Veterinary science

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14310/21:00129991

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-87294-98-7

ISSN

DOI

http://dx.doi.org/10.37904/nanocon.2020.3748

UT WoS

000664505500055

Klíčová slova anglicky

Emergency biosensor; nucleic acid; oligonucleotide; quantum dots; super paramagnetic nanoparticle

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 6. 2023 19:20, Mgr. Marie Šípková, DiS.

Anotace

V originále

African Swine Fever Virus (ASFV) is a DNA virus of the Asfivirus genus of the Asfarviridae family that is found in blood, body fluids, and internal organs. ASFV was described more than 40 years ago. This virus spreads pandemically and the mortality rate of the virus-related disease ranges from 90 to 100 %. The aim of this study was to propose the detection of specific nucleic acid of ASFV using electrochemical hybridization biosensor. Determination of DNA was conducted by AdTSV DPV a CV (potential 0 V, end potential -1.8 V, step potential 5 mV, modulation amplitude 25 mV, 0.2 M acetate buffer pH 5.0). The volume of analysed sample was 10 mu L. CV signals CA (log -0.0373x, r 0.99, Ep -1.30 V) and P peak (log -0.0801x, r 0.99, Ep -1.52 V) were observed. To increase the sensitivity, a modification of ODN with CdTe was proposed. The CdTe signal was observed around the potential of -0.56 V and for modified ODNs the signal was -0.58 V. SPION were prepared to capture DNA. The interaction of DNA (PCR fragment, 280 bp) with SPION was very fast within 30 s. The technique will be further used for a microfluidic system.

Citovat

BANÁŠ, Dominik, Ondřej RYCHLÝ, Stefano SALMISTRARO, Ahmet DAVUT AKSU, Miroslava KRZYŽÁNKOVÁ a René KIZEK. OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES. In Proceedings 12th International Conference on Nanomaterials - Research & Application. Ostrava: TANGER LTD, 2021, s. 324-329. ISBN 978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3748.

@inproceedings{1875861,
   author = {Banáš, Dominik and Rychlý, Ondřej and Salmistraro, Stefano and Davut Aksu, Ahmet and Krzyžánková, Miroslava and Kizek, René},
   address = {Ostrava},
   booktitle = {Proceedings 12th International Conference on Nanomaterials - Research & Application},
   doi = {http://dx.doi.org/10.37904/nanocon.2020.3748},
   keywords = {Emergency biosensor; nucleic acid; oligonucleotide; quantum dots; super paramagnetic nanoparticle},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Ostrava},
   isbn = {978-80-87294-98-7},
   pages = {324-329},
   publisher = {TANGER LTD},
   title = {OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES},
   url = {https://www.confer.cz/nanocon/2020/3748-optimization-of-nucleic-acid-binding-to-magnetic-particles-with-the-aim-of-detection-of-dangerous-viruses},
   year = {2021}
}

TY  - JOUR
ID  - 1875861
AU  - Banáš, Dominik - Rychlý, Ondřej - Salmistraro, Stefano - Davut Aksu, Ahmet - Krzyžánková, Miroslava - Kizek, René
PY  - 2021
TI  - OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES
PB  - TANGER LTD
CY  - Ostrava
SN  - 9788087294987
KW  - Emergency biosensor
KW  - nucleic acid
KW  - oligonucleotide
KW  - quantum dots
KW  - super paramagnetic nanoparticle
UR  - https://www.confer.cz/nanocon/2020/3748-optimization-of-nucleic-acid-binding-to-magnetic-particles-with-the-aim-of-detection-of-dangerous-viruses
N2  - African Swine Fever Virus (ASFV) is a DNA virus of the Asfivirus genus of the Asfarviridae family that is found in blood, body fluids, and internal organs. ASFV was described more than 40 years ago. This virus spreads pandemically and the mortality rate of the virus-related disease ranges from 90 to 100 %. The aim of this study was to propose the detection of specific nucleic acid of ASFV using electrochemical hybridization biosensor. Determination of DNA was conducted by AdTSV DPV a CV (potential 0 V, end potential -1.8 V, step potential 5 mV, modulation amplitude 25 mV, 0.2 M acetate buffer pH 5.0). The volume of analysed sample was 10 mu L. CV signals CA (log -0.0373x, r 0.99, Ep -1.30 V) and P peak (log -0.0801x, r 0.99, Ep -1.52 V) were observed. To increase the sensitivity, a modification of ODN with CdTe was proposed. The CdTe signal was observed around the potential of -0.56 V and for modified ODNs the signal was -0.58 V. SPION were prepared to capture DNA. The interaction of DNA (PCR fragment, 280 bp) with SPION was very fast within 30 s. The technique will be further used for a microfluidic system.
ER  -

BANÁŠ, Dominik, Ondřej RYCHLÝ, Stefano SALMISTRARO, Ahmet DAVUT AKSU, Miroslava KRZYŽÁNKOVÁ a René KIZEK. OPTIMIZATION OF NUCLEIC ACID BINDING TO MAGNETIC PARTICLES WITH THE AIM OF DETECTION OF DANGEROUS VIRUSES. In \textit{Proceedings 12th International Conference on Nanomaterials - Research \&{} Application}. Ostrava: TANGER LTD, 2021, s.~324-329. ISBN~978-80-87294-98-7. Dostupné z: https://dx.doi.org/10.37904/nanocon.2020.3748.

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