Cyanine Phototruncation Enables Spatiotemporal Cell Labeling

J 2022

Cyanine Phototruncation Enables Spatiotemporal Cell Labeling

FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO et. al.

Základní údaje

Originální název

Cyanine Phototruncation Enables Spatiotemporal Cell Labeling

Autoři

FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ (203 Česká republika, domácí), Petr KLÁN (203 Česká republika, garant, domácí), Hisataka KOBAYASHI a Martin J. SCHNERMANN

Vydání

Journal of the American Chemical Society, Washington, American Chemical Society, 2022, 0002-7863

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 15.000

Kód RIV

RIV/00216224:14310/22:00126376

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1021/jacs.2c02962

UT WoS

000820974300001

Klíčová slova anglicky

LYMPH-NODE; IN-VIVO; IDENTIFICATION; TRACKING; EGFP

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2023 23:13, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here we develop phototruncation-assisted cell tracking (PACT) and apply it to evaluate the migration of immune cells into tumor-draining lymphatics. This method is enabled by a recently discovered cyanine photoconversion reaction that leads to the two-carbon truncation and consequent blue-shift of these commonly used probes. By examining substituent effects on the heptamethine cyanine chromophore, we find that introduction of a single methoxy group increases the yield of the phototruncation reaction in neutral buffer by almost 8-fold. When converted to a membrane-bound cell-tracking variant, this probe can be applied in a series of in vitro and in vivo experiments. These include quantitative, time-dependent measurements of the migration of immune cells from tumors to tumor-draining lymph nodes. Unlike previously reported cellular photoconversion approaches, this method does not require genetic engineering and uses near-infrared (NIR) wavelengths. Overall, PACT provides a straightforward approach to label cell populations with spatiotemporal control.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

LM2018121, projekt VaV

Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

Citovat

FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ, Petr KLÁN, Hisataka KOBAYASHI a Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. Journal of the American Chemical Society. Washington: American Chemical Society, 2022, roč. 144, č. 25, s. 11075-11080. ISSN 0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.2c02962.

@article{1883610,
   author = {Fukushima, Hiroshi and Matikonda, Siddharth S. and Usama, Syed Muhammad and Furusawa, Aki and Kato, Takuya and Štacková, Lenka and Klán, Petr and Kobayashi, Hisataka and Schnermann, Martin J.},
   article_location = {Washington},
   article_number = {25},
   doi = {http://dx.doi.org/10.1021/jacs.2c02962},
   keywords = {LYMPH-NODE; IN-VIVO; IDENTIFICATION; TRACKING; EGFP},
   language = {eng},
   issn = {0002-7863},
   journal = {Journal of the American Chemical Society},
   title = {Cyanine Phototruncation Enables Spatiotemporal Cell Labeling},
   url = {https://pubs.acs.org/doi/10.1021/jacs.2c02962},
   volume = {144},
   year = {2022}
}

TY  - JOUR
ID  - 1883610
AU  - Fukushima, Hiroshi - Matikonda, Siddharth S. - Usama, Syed Muhammad - Furusawa, Aki - Kato, Takuya - Štacková, Lenka - Klán, Petr - Kobayashi, Hisataka - Schnermann, Martin J.
PY  - 2022
TI  - Cyanine Phototruncation Enables Spatiotemporal Cell Labeling
JF  - Journal of the American Chemical Society
VL  - 144
IS  - 25
SP  - 11075-11080
EP  - 11075-11080
PB  - American Chemical Society
SN  - 00027863
KW  - LYMPH-NODE
KW  - IN-VIVO
KW  - IDENTIFICATION
KW  - TRACKING
KW  - EGFP
UR  - https://pubs.acs.org/doi/10.1021/jacs.2c02962
N2  - Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here we develop phototruncation-assisted cell tracking (PACT) and apply it to evaluate the migration of immune cells into tumor-draining lymphatics. This method is enabled by a recently discovered cyanine photoconversion reaction that leads to the two-carbon truncation and consequent blue-shift of these commonly used probes. By examining substituent effects on the heptamethine cyanine chromophore, we find that introduction of a single methoxy group increases the yield of the phototruncation reaction in neutral buffer by almost 8-fold. When converted to a membrane-bound cell-tracking variant, this probe can be applied in a series of in vitro and in vivo experiments. These include quantitative, time-dependent measurements of the migration of immune cells from tumors to tumor-draining lymph nodes. Unlike previously reported cellular photoconversion approaches, this method does not require genetic engineering and uses near-infrared (NIR) wavelengths. Overall, PACT provides a straightforward approach to label cell populations with spatiotemporal control.
ER  -

FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ, Petr KLÁN, Hisataka KOBAYASHI a Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2022, roč.~144, č.~25, s.~11075-11080. ISSN~0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.2c02962.

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