| FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ, Petr KLÁN, Hisataka KOBAYASHI a Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. Journal of the American Chemical Society. Washington: American Chemical Society, 2022, roč. 144, č. 25, s. 11075-11080. ISSN 0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.2c02962. |
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@article{1883610,
author = {Fukushima, Hiroshi and Matikonda, Siddharth S. and Usama, Syed Muhammad and Furusawa, Aki and Kato, Takuya and Štacková, Lenka and Klán, Petr and Kobayashi, Hisataka and Schnermann, Martin J.},
article_location = {Washington},
article_number = {25},
doi = {http://dx.doi.org/10.1021/jacs.2c02962},
keywords = {LYMPH-NODE; IN-VIVO; IDENTIFICATION; TRACKING; EGFP},
language = {eng},
issn = {0002-7863},
journal = {Journal of the American Chemical Society},
title = {Cyanine Phototruncation Enables Spatiotemporal Cell Labeling},
url = {https://pubs.acs.org/doi/10.1021/jacs.2c02962},
volume = {144},
year = {2022}
}
TY - JOUR
ID - 1883610
AU - Fukushima, Hiroshi - Matikonda, Siddharth S. - Usama, Syed Muhammad - Furusawa, Aki - Kato, Takuya - Štacková, Lenka - Klán, Petr - Kobayashi, Hisataka - Schnermann, Martin J.
PY - 2022
TI - Cyanine Phototruncation Enables Spatiotemporal Cell Labeling
JF - Journal of the American Chemical Society
VL - 144
IS - 25
SP - 11075-11080
EP - 11075-11080
PB - American Chemical Society
SN - 00027863
KW - LYMPH-NODE
KW - IN-VIVO
KW - IDENTIFICATION
KW - TRACKING
KW - EGFP
UR - https://pubs.acs.org/doi/10.1021/jacs.2c02962
N2 - Photoconvertible tracking strategies assess the dynamic migration of cell populations. Here we develop phototruncation-assisted cell tracking (PACT) and apply it to evaluate the migration of immune cells into tumor-draining lymphatics. This method is enabled by a recently discovered cyanine photoconversion reaction that leads to the two-carbon truncation and consequent blue-shift of these commonly used probes. By examining substituent effects on the heptamethine cyanine chromophore, we find that introduction of a single methoxy group increases the yield of the phototruncation reaction in neutral buffer by almost 8-fold. When converted to a membrane-bound cell-tracking variant, this probe can be applied in a series of in vitro and in vivo experiments. These include quantitative, time-dependent measurements of the migration of immune cells from tumors to tumor-draining lymph nodes. Unlike previously reported cellular photoconversion approaches, this method does not require genetic engineering and uses near-infrared (NIR) wavelengths. Overall, PACT provides a straightforward approach to label cell populations with spatiotemporal control.
ER -
FUKUSHIMA, Hiroshi, Siddharth S. MATIKONDA, Syed Muhammad USAMA, Aki FURUSAWA, Takuya KATO, Lenka ŠTACKOVÁ, Petr KLÁN, Hisataka KOBAYASHI a Martin J. SCHNERMANN. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling. \textit{Journal of the American Chemical Society}. Washington: American Chemical Society, 2022, roč.~144, č.~25, s.~11075-11080. ISSN~0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.2c02962.
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