Specific Determination of Glutathione using Micellar
Elektrokinetic Chromatography and On-Column Reaction with
2,2-dipyridyldisulphide

D 1999

Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ

Basic information

Original name

Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide

Authors

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ

Edition

Amsterdam, Netherlands, Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands, p. 10-10, 1999

Publisher

Elsevier, Netherlands

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

is not subject to a state or trade secret

RIV identification code

RIV/00216224:14310/99:00003210

Organization unit

Faculty of Science

Keywords in English

Micellar Elektrokinetic Chromatography; On-Column Derivatization; Thiols; Glutathione

Abstract

In the original language

The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.

Links

GA525/97/0340, research and development project

Name: Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů

Investor: Czech Science Foundation, Activity of Thiobacillus ferrooxidans in relation to ecotoxicology of sulfide wastes

Cite

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide. In Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands. Amsterdam, Netherlands: Elsevier, Netherlands, 1999, p. 10.

@inproceedings{205215,
   author = {Glatz, Zdeněk and Mašlaňová, Hana},
   address = {Amsterdam, Netherlands},
   booktitle = {Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands},
   keywords = {Micellar Elektrokinetic Chromatography; On-Column Derivatization; Thiols; Glutathione},
   language = {eng},
   location = {Amsterdam, Netherlands},
   pages = {10-10},
   publisher = {Elsevier, Netherlands},
   title = {Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide},
   year = {1999}
}

TY  - CONF
ID  - 205215
AU  - Glatz, Zdeněk - Mašlaňová, Hana
PY  - 1999
TI  - Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide
PB  - Elsevier, Netherlands
CY  - Amsterdam, Netherlands
KW  - Micellar Elektrokinetic Chromatography
KW  - On-Column Derivatization
KW  - Thiols
KW  - Glutathione
N2  - The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.
ER  -

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Specific Determination of Glutathione using Micellar Elektrokinetic Chromatography and On-Column Reaction with 2,2-dipyridyldisulphide. In \textit{Book of Abstract, 1st International Symposium on Separations in the Biosciences, Amsterdam, Netherlands}. Amsterdam, Netherlands: Elsevier, Netherlands, 1999, p.~10.

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