Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography

GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography. In Proceeding of 13th International Conference. Bratislava: Slovenská chemická společnosť pri SAV, Bratislava, 1999, p. 25-26. ISBN 1335-5236.

Basic information

• Original name: Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography
• Authors: GLATZ, Zdeněk and Hana MAŠLAŇOVÁ.
• Edition: Bratislava, Proceeding of 13th International Conference, p. 25-26, 1999.
• Publisher: Slovenská chemická společnosť pri SAV, Bratislava

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/99:00003221
• Organization unit: Faculty of Science
• ISBN: 1335-5236
• Keywords in English: Glutathione; MEKC; 2;2-dipyridyldisulphide
• Tags: 2, 2-dipyridyldisulphide, Glutathione, MEKC

Abstract

The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.

Links

• GA525/97/0340, research and development project: Name: Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů

Investor: Czech Science Foundation, Activity of Thiobacillus ferrooxidans in relation to ecotoxicology of sulfide wastes