Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar ...
|GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography. In Proceeding of 13th International Conference. Bratislava: Slovenská chemická společnosť pri SAV, Bratislava, 1999. p. 25-26. ISBN 1335-5236.
|Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography
|GLATZ, Zdeněk and Hana MAŠLAŇOVÁ.
|Bratislava, Proceeding of 13th International Conference, p. 25-26, 1999.
|Slovenská chemická společnosť pri SAV, Bratislava
|Type of outcome
|Field of Study
|10600 1.6 Biological sciences
|Country of publisher
|is not subject to a state or trade secret
|RIV identification code
|Faculty of Science
|Keywords in English
|Glutathione; MEKC; 2;2-dipyridyldisulphide
|2, 2-dipyridyldisulphide, Glutathione, MEKC
|Changed by: prof. RNDr. Zdeněk Glatz, CSc., učo 1865. Changed: 28/2/2001 10:00.
|The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.
|GA525/97/0340, research and development project
|Name: Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů
|Investor: Czech Science Foundation, Activity of Thiobacillus ferrooxidans in relation to ecotoxicology of sulfide wastes
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