GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography. In Proceeding of 13th International Conference. Bratislava: Slovenská chemická společnosť pri SAV, Bratislava, 1999. p. 25-26. ISBN 1335-5236.
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Basic information
Original name Application of 2,2-Dipyridyldisulphide for Specific Determination of Glutathione during Micellar Electrokinetic Chromatography
Authors GLATZ, Zdeněk and Hana MAŠLAŇOVÁ.
Edition Bratislava, Proceeding of 13th International Conference, p. 25-26, 1999.
Publisher Slovenská chemická společnosť pri SAV, Bratislava
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Slovakia
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/99:00003221
Organization unit Faculty of Science
ISBN 1335-5236
Keywords in English Glutathione; MEKC; 2;2-dipyridyldisulphide
Tags 2, 2-dipyridyldisulphide, Glutathione, MEKC
Changed by Changed by: prof. RNDr. Zdeněk Glatz, CSc., učo 1865. Changed: 28/2/2001 10:00.
The on-column reaction of glutathione with the reactive disulphide 2,2-dipyridyldisulphide was used for the specific detection of glutathione during micellar electrokinetic chromatography: In this set-up the front end of the capillary is used as the reaction chamber. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively in the capillary as two discrete plugs separated with short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione on column mixing and reaction occur. This reaction is rapid and quantitative, because thiol form is stabilised by thiol-thione tautomerism. This fact also causes a large shift of the absorption maxima toward longer wavelength. Glutathione is thus quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amounts of the 2-thiopyridone that is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm.
GA525/97/0340, research and development projectName: Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů
Investor: Czech Science Foundation, Activity of Thiobacillus ferrooxidans in relation to ecotoxicology of sulfide wastes
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