Sensitive amperometric biosensor for the determination of
biogenic and synthetic amines using pea seedling amine oxidase:
a novel approach for enzyme immobilisation

J 1999

Sensitive amperometric biosensor for the determination of biogenic and synthetic amines using pea seedling amine oxidase: a novel approach for enzyme immobilisation

WIMMEROVÁ, Michaela a Lumír MACHOLÁN

Základní údaje

Originální název

Sensitive amperometric biosensor for the determination of biogenic and synthetic amines using pea seedling amine oxidase: a novel approach for enzyme immobilisation

Autoři

WIMMEROVÁ, Michaela (203 Česká republika, garant) a Lumír MACHOLÁN (203 Česká republika)

Vydání

Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 1999, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.020

Kód RIV

RIV/00216224:14310/99:00008547

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

biosensor; amine oxidase; immobilisation

Štítky

amine oxidase, biosensor, immobilisation

Změněno: 11. 3. 2004 16:15, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

V originále

We prepared a new inorganic sorbent based on modified triazine (2-[4,6-bis (aminoethylamine)-1,3,5-triazine]-Silasorb; BAT-Silasorb) which binds pea seedlings amine oxidase (PSAO) very tightly without loss of its catalytic activity. This unique feature as well as the wide substrate specificity of PSAO was successfully utilised in the construction of an amperometric biosensor based on a carbon paste electrode for the fast and sensitive detection of various amines at a formal potential 0 mV versus Ag:AgCl reference electrode. The reaction layer of the biosensor is created by the direct immobilisation of PSAO at the electrode surface via affinity carrier BAT-Silasorb. Used arrangement facilitates a simple restoration of the inactive biosensor. An amperometric signal results from horseradish peroxidase catalysed reduction of H2O2, a secondary product of the oxidative deamination of amines, catalysed by PSAO. The sensor was used for the basic characterisation of 55 biogenic and synthetic amines, from numerous mono-, di- and polyamines to various hydroxy-, thio-, benzyl- and aromatic derivatives in order to establish its suitability as a postcolumn detector. Its high sensitivity to putrescine, a limit of detection of 10 nmol/l (determined with respect to a signal-to-noise ratio 3:1), a linear range of current response to 0.01-100 mmol/l concentration of substrate and good reproducibility all indicate that the sensor could be applied to future industrial and clinical analyses.

Návaznosti

MSM 143100005, záměr

Název: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Citovat

WIMMEROVÁ, Michaela a Lumír MACHOLÁN. Sensitive amperometric biosensor for the determination of biogenic and synthetic amines using pea seedling amine oxidase: a novel approach for enzyme immobilisation. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 1999, roč. 14, 8-9, s. 695-702. ISSN 0956-5663.

@article{214772,
   author = {Wimmerová, Michaela and Macholán, Lumír},
   article_location = {Oxford},
   article_number = {8-9},
   keywords = {biosensor; amine oxidase; immobilisation},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors & Bioelectronics},
   title = {Sensitive amperometric biosensor for the determination of biogenic and synthetic amines using pea seedling amine oxidase: a novel approach for enzyme immobilisation},
   volume = {14},
   year = {1999}
}

TY  - JOUR
ID  - 214772
AU  - Wimmerová, Michaela - Macholán, Lumír
PY  - 1999
TI  - Sensitive amperometric biosensor for the determination of biogenic and synthetic amines using pea seedling amine oxidase: a novel approach for enzyme immobilisation
JF  - Biosensors & Bioelectronics
VL  - 14
IS  - 8-9
SP  - 695
EP  - 695
PB  - Elsevier Advanced Technology
SN  - 09565663
KW  - biosensor
KW  - amine oxidase
KW  - immobilisation
N2  - We prepared a new inorganic sorbent based on modified triazine (2-[4,6-bis (aminoethylamine)-1,3,5-triazine]-Silasorb; BAT-Silasorb) which binds pea seedlings amine oxidase (PSAO) very tightly without loss of its catalytic activity. This unique feature as well as the wide substrate specificity of PSAO was successfully utilised in the construction of an amperometric biosensor based on a carbon paste electrode for the fast and sensitive detection of various amines at a formal potential 0 mV versus Ag:AgCl reference electrode. The reaction layer of the biosensor is created by the direct immobilisation of PSAO at the electrode surface via affinity carrier BAT-Silasorb. Used arrangement facilitates a simple restoration of the inactive biosensor. An amperometric signal results from horseradish peroxidase catalysed reduction of H2O2, a secondary product of the oxidative deamination of amines, catalysed by PSAO. The sensor was used for the basic characterisation of 55 biogenic and synthetic amines, from numerous mono-, di- and polyamines to various hydroxy-, thio-, benzyl- and aromatic derivatives in order to establish its suitability as a postcolumn detector. Its high sensitivity to putrescine, a limit of detection of 10 nmol/l (determined with respect to a signal-to-noise ratio 3:1), a linear range of current response to 0.01-100 mmol/l concentration of substrate and good reproducibility all indicate that the sensor could be applied to future industrial and clinical analyses.
ER  -

WIMMEROVÁ, Michaela a Lumír MACHOLÁN. Sensitive amperometric biosensor for the determination of biogenic and synthetic amines using pea seedling amine oxidase: a novel approach for enzyme immobilisation. \textit{Biosensors \&{} Bioelectronics}. Oxford: Elsevier Advanced Technology, 1999, roč.~14, 8-9, s.~695-702. ISSN~0956-5663.

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