Podrobný výpis o publikaci

PREISLER, J. a E. S. YEUNG. Laser Photodissociation of Insulin Ions Generated by Matrix-Assisted Laser Desorption. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 1997, roč. 69, č. 21, s. 4390-4398. ISSN 0003-2700.

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Základní údaje
Originální název	Laser Photodissociation of Insulin Ions Generated by Matrix-Assisted Laser Desorption
Název česky	Laserová fotodisociace insulinových iontů tvořených laserovou desorpcí za účasti matrice
Autoři	PREISLER, J. (203 Česká republika, garant) a E. S. YEUNG (840 Spojené státy).
Vydání	Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 1997, 0003-2700.

Další údaje
Originální jazyk	angličtina
Typ výsledku	Článek v odborném periodiku
Obor	10406 Analytical chemistry
Stát vydavatele	Spojené státy
Utajení	není předmětem státního či obchodního tajemství
Impakt faktor	Impact factor: 4.743
Organizační jednotka	Přírodovědecká fakulta
UT WoS	A1997YD67800020
Klíčová slova anglicky	laser; photodissociation; MALDI; matrix; desorption; insulin;
Štítky	Desorption, insulin, laser, MALDI, matrix, photodissociation
Změnil	Změnil: prof. Mgr. Jan Preisler, Ph.D., učo 45329. Změněno: 28. 6. 2009 00:03.

Anotace
A novel method for studying the ionization step of matrixassisted laser desorption/ionization (MALDI) is demonstrated. A 193-nm pulse from an ArF excimer laser is used to photodissociate a portion of a plume of insulin ions generated by MALDI. Laser photodissociation (LPD) creates a "hole", i.e., a negative spike in the insulin peak in the time-of-flight (TOF) mass spectrum. The position of the hole in the mass spectrum provides useful measurements of the characteristics (position, time, and velocity) of insulin ions shortly after their creation. Although the performance of the method can be further improved, the data obtained could be used to refine our current understanding of MALDI and to improve the resolution of MALDI-TOFMS.

Anotace

A novel method for studying the ionization step of matrixassisted laser desorption/ionization (MALDI) is demonstrated. A 193-nm pulse from an ArF excimer laser is used to photodissociate a portion of a plume of insulin ions generated by MALDI. Laser photodissociation (LPD) creates a "hole", i.e., a negative spike in the insulin peak in the time-of-flight (TOF) mass spectrum. The position of the hole in the mass spectrum provides useful measurements of the characteristics (position, time, and velocity) of insulin ions shortly after their creation. Although the performance of the method can be further improved, the data obtained could be used to refine our current understanding of MALDI and to improve the resolution of MALDI-TOFMS.