Topology of microtubules and actin in the life cycle of
Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

J 1999

Topology of microtubules and actin in the life cycle of Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

SLANINOVÁ, Iva, Judit KUCSERA a Augustin SVOBODA

Základní údaje

Originální název

Topology of microtubules and actin in the life cycle of Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

Autoři

SLANINOVÁ, Iva (203 Česká republika), Judit KUCSERA a Augustin SVOBODA (203 Česká republika)

Vydání

Antonie van Leeuwenhoek, Dordrecht (The Netherlands), Kluwer Academic Publishers, 1999, 0003-6072

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.423

Kód RIV

RIV/00216224:14110/99:00001302

Organizační jednotka

Lékařská fakulta

UT WoS

000082271500011

Klíčová slova anglicky

actin

Štítky

Actin

Změněno: 6. 11. 2003 17:34, prof. MUDr. Iva Slaninová, Ph.D.

Anotace

V originále

The morphology of budding and conjugating cells and associated changes in microtubules and actin distribution were studied in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) by phase-contrast and fluorescence microscopy. The non-budding interphase cell showed a nucleus situated in the central position and bundles of cytoplasmic microtubules either stretching parallel to the longitudinal cell axis or randomly distributed in the cell; none of these, however, were in apparent contact with the nucleus. During mitosis, the nucleus divided in the daughter cell, cytoplasmic microtubules disappeared and were replaced by a spindle. The cytoplasmic microtubules reappeared after mitosis had finished. Actin patches were present both in the bud and the mother cell. Cells were induced to mate by nitrogen depletion. Partner cells fused by conjugation projections where actin patches had been accumulated. Cell fusion resulted in a zygote that produced a holobasidium with parallel bundles of microtubules extended along the basidiophore axis and with actin patches concentrated at the apex. The fused nucleus moved towards the tip of the basidiophore . During this movement, nuclear division was taking place; the nuclei were eventually distributed to basidiospores. Mitochondria visualised by means of DIOC6(3) appeared as vesicles of various sizes; their large amounts were found, often lying adjacent to microtubules, in the cytoplasm of both vegetative cells and zygotes.

Návaznosti

GA204/97/P097, projekt VaV

Název: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Grantová agentura ČR, Úloha cytoskeletu a buněčné stěny v polarizaci buňky

GA204/97/1150, projekt VaV

Název: Buněčná stěna a morfogeneze kvasinkové buňky: role cytoskeletu

Investor: Grantová agentura ČR, Buněčná stěna a morfogeneze kvasinkové buňky: role cytoskeletu

Citovat

SLANINOVÁ, Iva, Judit KUCSERA a Augustin SVOBODA. Topology of microtubules and actin in the life cycle of Xanthophyllomyces dendrorhous (Phaffia rhodozyma). Antonie van Leeuwenhoek. Dordrecht (The Netherlands): Kluwer Academic Publishers, 1999, roč. 75, č. 4, s. 361-368. ISSN 0003-6072.

@article{219331,
   author = {Slaninová, Iva and Kucsera, Judit and Svoboda, Augustin},
   article_location = {Dordrecht (The Netherlands)},
   article_number = {4},
   keywords = {actin},
   language = {eng},
   issn = {0003-6072},
   journal = {Antonie van Leeuwenhoek},
   title = {Topology of microtubules and actin in the life cycle of Xanthophyllomyces dendrorhous (Phaffia rhodozyma)},
   volume = {75},
   year = {1999}
}

TY  - JOUR
ID  - 219331
AU  - Slaninová, Iva - Kucsera, Judit - Svoboda, Augustin
PY  - 1999
TI  - Topology of microtubules and actin in the life cycle of Xanthophyllomyces dendrorhous (Phaffia rhodozyma)
JF  - Antonie van Leeuwenhoek
VL  - 75
IS  - 4
SP  - 361
EP  - 361
PB  - Kluwer Academic Publishers
SN  - 00036072
KW  - actin
N2  - The morphology of budding and conjugating cells and associated changes in microtubules and actin distribution were studied in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) by phase-contrast and fluorescence microscopy. The non-budding interphase cell showed a nucleus situated in the central position and bundles of cytoplasmic microtubules either stretching parallel to the longitudinal cell axis or randomly distributed in the cell; none of these, however, were in apparent contact with the nucleus. During mitosis, the nucleus divided in the daughter cell, cytoplasmic microtubules disappeared and were replaced by a spindle. The cytoplasmic microtubules reappeared after mitosis had finished. Actin patches were present both in the bud and the mother cell. Cells were induced to mate by nitrogen depletion. Partner cells fused by conjugation projections where actin patches had been accumulated. Cell fusion resulted in a zygote that produced a holobasidium with parallel bundles of microtubules extended along the basidiophore axis and with actin patches concentrated at the apex. The fused nucleus moved towards the tip of the basidiophore . During this movement, nuclear division was taking place; the nuclei were eventually distributed to basidiospores. Mitochondria visualised by means of DIOC6(3) appeared as vesicles of various sizes; their large amounts were found, often lying adjacent to microtubules, in the cytoplasm of both vegetative cells and zygotes.
ER  -

SLANINOVÁ, Iva, Judit KUCSERA a Augustin SVOBODA. Topology of microtubules and actin in the life cycle of Xanthophyllomyces dendrorhous (Phaffia rhodozyma). \textit{Antonie van Leeuwenhoek}. Dordrecht (The Netherlands): Kluwer Academic Publishers, 1999, roč.~75, č.~4, s.~361-368. ISSN~0003-6072.

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