Macrophage-mediated tissue response evoked by subchronic
inhalation of lead oxide nanoparticles is associated with the
alteration of phospholipases C and cholesterol transporters

J 2022

Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters

SMUTNÁ, Tereza, Jana DUMKOVÁ, Daniela KRISTEKOVÁ, Markéta LAŠTOVIČKOVÁ, Adriena JEDLIČKOVÁ et. al.

Základní údaje

Originální název

Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters

Autoři

SMUTNÁ, Tereza (203 Česká republika), Jana DUMKOVÁ (203 Česká republika, domácí), Daniela KRISTEKOVÁ (703 Slovensko, domácí), Markéta LAŠTOVIČKOVÁ (203 Česká republika), Adriena JEDLIČKOVÁ (203 Česká republika), Lucie VRLÍKOVÁ (203 Česká republika), Bohumil DOČEKAL (203 Česká republika), Lukáš ALEXA (203 Česká republika), Hana KOTASOVÁ (203 Česká republika, domácí), Vendula PELKOVÁ (203 Česká republika, domácí), Zbyněk VEČEŘA (203 Česká republika), Kamil KŘŮMAL (203 Česká republika), Jiří PETRÁŠ (203 Česká republika), Pavel COUFALÍK (203 Česká republika), Dalibor VŠIANSKÝ (203 Česká republika, domácí), Samuel ZÁCHEJ (203 Česká republika), Dominik PINKAS (203 Česká republika), Jan VONDRÁČEK (203 Česká republika), Aleš HAMPL (203 Česká republika, domácí), Pavel MIKUŠKA (203 Česká republika) a Marcela BUCHTOVÁ (203 Česká republika, garant, domácí)

Vydání

Particle and Fibre Toxicology, London, BioMed Central, 2022, 1743-8977

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30108 Toxicology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.000

Kód RIV

RIV/00216224:14310/22:00126406

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1186/s12989-022-00494-7

UT WoS

000835708500001

Klíčová slova anglicky

Cholesterol metabolism; Inhalation; Lead oxide nanoparticles; Liver macrophages; Lung macrophages.

Štítky

14110517, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2023 13:02, Mgr. Marie Šípková, DiS.

Anotace

V originále

Background: Inhalation of lead oxide nanoparticles (PbO NPs), which are emitted to the environment by high-temperature technological processes, heavily impairs target organs. These nanoparticles pass through the lung barrier and are distributed via the blood into secondary target organs, where they cause numerous pathological alterations. Here, we studied in detail, macrophages as specialized cells involved in the innate and adaptive immune response in selected target organs to unravel their potential involvement in reaction to subchronic PbO NP inhalation. In this context, we also tackled possible alterations in lipid uptake in the lungs and liver, which is usually associated with foam macrophage formation. Results: The histopathological analysis of PbO NP exposed lung revealed serious chronic inflammation of lung tissues. The number of total and foam macrophages was significantly increased in lung, and they contained numerous cholesterol crystals. PbO NP inhalation induced changes in expression of phospholipases C (PLC) as enzymes linked to macrophage-mediated inflammation in lungs. In the liver, the subchronic inhalation of PbO NPs caused predominantly hyperemia, microsteatosis or remodeling of the liver parenchyma, and the number of liver macrophages also significantly was increased. The gene and protein expression of a cholesterol transporter CD36, which is associated with lipid metabolism, was altered in the liver. The amount of selected cholesteryl esters (CE 16:0, CE 18:1, CE 20:4, CE 22:6) in liver tissue was decreased after subchronic PbO NP inhalation, while total and free cholesterol in liver tissue was slightly increased. Gene and protein expression of phospholipase PLCβ1 and receptor CD36 in human hepatocytes were affected also in in vitro experiments after acute PbO NP exposure. No microscopic or serious functional kidney alterations were detected after subchronic PbO NP exposure and CD68 positive cells were present in the physiological mode in its interstitial tissues. Conclusion: Our study revealed the association of increased cholesterol and lipid storage in targeted tissues with the alteration of scavenger receptors and phospholipases C after subchronic inhalation of PbO NPs and yet uncovered processes, which can contribute to steatosis in liver after metal nanoparticles exposure.

Návaznosti

GA20-02203S, projekt VaV

Název: Analýza tkáňové odpovědi na inhalaci nanočástic kovů a mechanismus jejich čištění

Investor: Grantová agentura ČR, Analysis of tissue response to metal nanoparticles inhalation and mechanisms of their clearance

Citovat

SMUTNÁ, Tereza, Jana DUMKOVÁ, Daniela KRISTEKOVÁ, Markéta LAŠTOVIČKOVÁ, Adriena JEDLIČKOVÁ, Lucie VRLÍKOVÁ, Bohumil DOČEKAL, Lukáš ALEXA, Hana KOTASOVÁ, Vendula PELKOVÁ, Zbyněk VEČEŘA, Kamil KŘŮMAL, Jiří PETRÁŠ, Pavel COUFALÍK, Dalibor VŠIANSKÝ, Samuel ZÁCHEJ, Dominik PINKAS, Jan VONDRÁČEK, Aleš HAMPL, Pavel MIKUŠKA a Marcela BUCHTOVÁ. Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters. Particle and Fibre Toxicology. London: BioMed Central, 2022, roč. 19, č. 1, s. 1-28. ISSN 1743-8977. Dostupné z: https://dx.doi.org/10.1186/s12989-022-00494-7.

@article{2210926,
   author = {Smutná, Tereza and Dumková, Jana and Kristeková, Daniela and Laštovičková, Markéta and Jedličková, Adriena and Vrlíková, Lucie and Dočekal, Bohumil and Alexa, Lukáš and Kotasová, Hana and Pelková, Vendula and Večeřa, Zbyněk and Křůmal, Kamil and Petráš, Jiří and Coufalík, Pavel and Všianský, Dalibor and Záchej, Samuel and Pinkas, Dominik and Vondráček, Jan and Hampl, Aleš and Mikuška, Pavel and Buchtová, Marcela},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s12989-022-00494-7},
   keywords = {Cholesterol metabolism; Inhalation; Lead oxide nanoparticles; Liver macrophages; Lung macrophages.},
   language = {eng},
   issn = {1743-8977},
   journal = {Particle and Fibre Toxicology},
   title = {Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters},
   url = {https://doi.org/10.1186/s12989-022-00494-7},
   volume = {19},
   year = {2022}
}

TY  - JOUR
ID  - 2210926
AU  - Smutná, Tereza - Dumková, Jana - Kristeková, Daniela - Laštovičková, Markéta - Jedličková, Adriena - Vrlíková, Lucie - Dočekal, Bohumil - Alexa, Lukáš - Kotasová, Hana - Pelková, Vendula - Večeřa, Zbyněk - Křůmal, Kamil - Petráš, Jiří - Coufalík, Pavel - Všianský, Dalibor - Záchej, Samuel - Pinkas, Dominik - Vondráček, Jan - Hampl, Aleš - Mikuška, Pavel - Buchtová, Marcela
PY  - 2022
TI  - Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters
JF  - Particle and Fibre Toxicology
VL  - 19
IS  - 1
SP  - 1-28
EP  - 1-28
PB  - BioMed Central
SN  - 17438977
KW  - Cholesterol metabolism
KW  - Inhalation
KW  - Lead oxide nanoparticles
KW  - Liver macrophages
KW  - Lung macrophages.
UR  - https://doi.org/10.1186/s12989-022-00494-7
N2  - Background: Inhalation of lead oxide nanoparticles (PbO NPs), which are emitted to the environment by high-temperature technological processes, heavily impairs target organs. These nanoparticles pass through the lung barrier and are distributed via the blood into secondary target organs, where they cause numerous pathological alterations. Here, we studied in detail, macrophages as specialized cells involved in the innate and adaptive immune response in selected target organs to unravel their potential involvement in reaction to subchronic PbO NP inhalation. In this context, we also tackled possible alterations in lipid uptake in the lungs and liver, which is usually associated with foam macrophage formation. Results: The histopathological analysis of PbO NP exposed lung revealed serious chronic inflammation of lung tissues. The number of total and foam macrophages was significantly increased in lung, and they contained numerous cholesterol crystals. PbO NP inhalation induced changes in expression of phospholipases C (PLC) as enzymes linked to macrophage-mediated inflammation in lungs. In the liver, the subchronic inhalation of PbO NPs caused predominantly hyperemia, microsteatosis or remodeling of the liver parenchyma, and the number of liver macrophages also significantly was increased. The gene and protein expression of a cholesterol transporter CD36, which is associated with lipid metabolism, was altered in the liver. The amount of selected cholesteryl esters (CE 16:0, CE 18:1, CE 20:4, CE 22:6) in liver tissue was decreased after subchronic PbO NP inhalation, while total and free cholesterol in liver tissue was slightly increased. Gene and protein expression of phospholipase PLCβ1 and receptor CD36 in human hepatocytes were affected also in in vitro experiments after acute PbO NP exposure. No microscopic or serious functional kidney alterations were detected after subchronic PbO NP exposure and CD68 positive cells were present in the physiological mode in its interstitial tissues. Conclusion: Our study revealed the association of increased cholesterol and lipid storage in targeted tissues with the alteration of scavenger receptors and phospholipases C after subchronic inhalation of PbO NPs and yet uncovered processes, which can contribute to steatosis in liver after metal nanoparticles exposure.
ER  -

SMUTNÁ, Tereza, Jana DUMKOVÁ, Daniela KRISTEKOVÁ, Markéta LAŠTOVIČKOVÁ, Adriena JEDLIČKOVÁ, Lucie VRLÍKOVÁ, Bohumil DOČEKAL, Lukáš ALEXA, Hana KOTASOVÁ, Vendula PELKOVÁ, Zbyněk VEČEŘA, Kamil KŘŮMAL, Jiří PETRÁŠ, Pavel COUFALÍK, Dalibor VŠIANSKÝ, Samuel ZÁCHEJ, Dominik PINKAS, Jan VONDRÁČEK, Aleš HAMPL, Pavel MIKUŠKA a Marcela BUCHTOVÁ. Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters. \textit{Particle and Fibre Toxicology}. London: BioMed Central, 2022, roč.~19, č.~1, s.~1-28. ISSN~1743-8977. Dostupné z: https://dx.doi.org/10.1186/s12989-022-00494-7.

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