Sequencing Independent Molecular Typing of Staphylococcus
aureus Isolates: Approach for Infection Control and Clonal
Characterization

J 2022

Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization

DUFKOVÁ, Kristýna, Matěj BEZDÍČEK, Kristina CUPROVA, Dagmar PANTUCKOVA, Marketa NYKRYNOVA et. al.

Základní údaje

Originální název

Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization

Autoři

DUFKOVÁ, Kristýna (203 Česká republika, domácí), Matěj BEZDÍČEK (203 Česká republika, domácí), Kristina CUPROVA (203 Česká republika), Dagmar PANTUCKOVA (203 Česká republika), Marketa NYKRYNOVA (203 Česká republika), Eva BRHELOVA (203 Česká republika), Iva KOCMANOVA (203 Česká republika), Silvie HODOVA (203 Česká republika), Marketa HANSLIANOVA (203 Česká republika), Tomas JUREN (203 Česká republika), Břetislav LIPOVÝ (203 Česká republika), Jiří MAYER (203 Česká republika, domácí) a Martina LENGEROVÁ (203 Česká republika, garant, domácí)

Vydání

Microbiology Spectrum, WASHINGTON, American Society for Microbiology, 2022, 2165-0497

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700

Kód RIV

RIV/00216224:14110/22:00126414

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1128/spectrum.01817-21

UT WoS

000766015800131

Klíčová slova anglicky

MLST; MRSA; MSSA; high-resolution melting; mini-MLST; spa-typing; whole-genome sequencing

Štítky

14110212, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2023 08:17, Mgr. Tereza Miškechová

Anotace

V originále

Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of clinical manifestations. The main aim of the presented study was to determine and optimize a novel sequencing independent approach that enables molecular typing of S. aureus isolates and elucidates the transmission of emergent clones between patients. In total, 987 S. aureus isolates including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were used to evaluate the novel typing approach combining high-resolution melting (HRM) analysis of multilocus sequence typing (MLST) genes (mini-MLST) and spa gene (spa-HRM). The novel approach's discriminatory ability was evaluated by whole-genome sequencing (WGS). The clonal relatedness of tested isolates was set by the BURP and BURST approach using spa and MLST data, respectively. Mini-MLST classified the S. aureus isolates into 38 clusters, followed by spa-HRM classifying the isolates into 101 clusters. The WGS proved HRM-based methods to effectively differentiate between related S. aureus isolates. Visualizing evolutionary relationships among different spa-types provided by the BURP algorithm showed comparable results to MLST/mini-MLST clonal clusters. We proved that the combination of mini-MLST and spa-HRM is rapid, reproducible, and cost-efficient. In addition to high discriminatory ability, the correlation between spa evolutionary relationships and mini-MLST clustering allows the variability in population structure to be monitored. IMPORTANCE Rapid and cost-effective molecular typing tools for Staphylococcus aureus epidemiological applications such as transmission tracking, source attribution and outbreak investigations are highly desirable. High-resolution melting based methods are effective alternative to those based on sequencing. Their good reproducibility and easy performance allow prospective typing of large set of isolates while reaching great discriminatory power. In this study, we established a new epidemiological approach to S. aureus typing. This scheme has the potential to greatly improve epidemiological investigations of S. aureus.

Návaznosti

EF19_073/0016943, projekt VaV

Název: Interní grantová agentura Masarykovy univerzity

MUNI/A/1330/2021, interní kód MU

Název: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX (Akronym: VýDiTeHeMa IX)

Investor: Masarykova univerzita, Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX

MUNI/IGA/1345/2020, interní kód MU

Název: The Development and Application of Fast MLST-based Typing Method for Multi-drug Resistant Bacteria Outbreak Detection

Investor: Masarykova univerzita, The Development and Application of Fast MLST-based Typing Method for Multi-drug Resistant Bacteria Outbreak Detection

Citovat

DUFKOVÁ, Kristýna, Matěj BEZDÍČEK, Kristina CUPROVA, Dagmar PANTUCKOVA, Marketa NYKRYNOVA, Eva BRHELOVA, Iva KOCMANOVA, Silvie HODOVA, Marketa HANSLIANOVA, Tomas JUREN, Břetislav LIPOVÝ, Jiří MAYER a Martina LENGEROVÁ. Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization. Microbiology Spectrum. WASHINGTON: American Society for Microbiology, 2022, roč. 10, č. 1, s. 1-10. ISSN 2165-0497. Dostupné z: https://dx.doi.org/10.1128/spectrum.01817-21.

@article{2211021,
   author = {Dufková, Kristýna and Bezdíček, Matěj and Cuprova, Kristina and Pantuckova, Dagmar and Nykrynova, Marketa and Brhelova, Eva and Kocmanova, Iva and Hodova, Silvie and Hanslianova, Marketa and Juren, Tomas and Lipový, Břetislav and Mayer, Jiří and Lengerová, Martina},
   article_location = {WASHINGTON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1128/spectrum.01817-21},
   keywords = {MLST; MRSA; MSSA; high-resolution melting; mini-MLST; spa-typing; whole-genome sequencing},
   language = {eng},
   issn = {2165-0497},
   journal = {Microbiology Spectrum},
   title = {Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization},
   url = {https://journals.asm.org/doi/10.1128/spectrum.01817-21},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 2211021
AU  - Dufková, Kristýna - Bezdíček, Matěj - Cuprova, Kristina - Pantuckova, Dagmar - Nykrynova, Marketa - Brhelova, Eva - Kocmanova, Iva - Hodova, Silvie - Hanslianova, Marketa - Juren, Tomas - Lipový, Břetislav - Mayer, Jiří - Lengerová, Martina
PY  - 2022
TI  - Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization
JF  - Microbiology Spectrum
VL  - 10
IS  - 1
SP  - 1-10
EP  - 1-10
PB  - American Society for Microbiology
SN  - 21650497
KW  - MLST
KW  - MRSA
KW  - MSSA
KW  - high-resolution melting
KW  - mini-MLST
KW  - spa-typing
KW  - whole-genome sequencing
UR  - https://journals.asm.org/doi/10.1128/spectrum.01817-21
N2  - Staphylococcus aureus is a major bacterial human pathogen that causes a wide variety of clinical manifestations. The main aim of the presented study was to determine and optimize a novel sequencing independent approach that enables molecular typing of S. aureus isolates and elucidates the transmission of emergent clones between patients. In total, 987 S. aureus isolates including both methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were used to evaluate the novel typing approach combining high-resolution melting (HRM) analysis of multilocus sequence typing (MLST) genes (mini-MLST) and spa gene (spa-HRM). The novel approach's discriminatory ability was evaluated by whole-genome sequencing (WGS). The clonal relatedness of tested isolates was set by the BURP and BURST approach using spa and MLST data, respectively. Mini-MLST classified the S. aureus isolates into 38 clusters, followed by spa-HRM classifying the isolates into 101 clusters. The WGS proved HRM-based methods to effectively differentiate between related S. aureus isolates. Visualizing evolutionary relationships among different spa-types provided by the BURP algorithm showed comparable results to MLST/mini-MLST clonal clusters. We proved that the combination of mini-MLST and spa-HRM is rapid, reproducible, and cost-efficient. In addition to high discriminatory ability, the correlation between spa evolutionary relationships and mini-MLST clustering allows the variability in population structure to be monitored. IMPORTANCE Rapid and cost-effective molecular typing tools for Staphylococcus aureus epidemiological applications such as transmission tracking, source attribution and outbreak investigations are highly desirable. High-resolution melting based methods are effective alternative to those based on sequencing. Their good reproducibility and easy performance allow prospective typing of large set of isolates while reaching great discriminatory power. In this study, we established a new epidemiological approach to S. aureus typing. This scheme has the potential to greatly improve epidemiological investigations of S. aureus.
ER  -

DUFKOVÁ, Kristýna, Matěj BEZDÍČEK, Kristina CUPROVA, Dagmar PANTUCKOVA, Marketa NYKRYNOVA, Eva BRHELOVA, Iva KOCMANOVA, Silvie HODOVA, Marketa HANSLIANOVA, Tomas JUREN, Břetislav LIPOVÝ, Jiří MAYER a Martina LENGEROVÁ. Sequencing Independent Molecular Typing of Staphylococcus aureus Isolates: Approach for Infection Control and Clonal Characterization. \textit{Microbiology Spectrum}. WASHINGTON: American Society for Microbiology, 2022, roč.~10, č.~1, s.~1-10. ISSN~2165-0497. Dostupné z: https://dx.doi.org/10.1128/spectrum.01817-21.

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