Measurement of Liver Stiffness using Atomic Force Microscopy
Coupled with Polarization Microscopy

J 2022

Measurement of Liver Stiffness using Atomic Force Microscopy Coupled with Polarization Microscopy

OJHA, Srikant; Jan PŘIBYL; Šimon KLIMOVIČ; Daniel HADRABA; Marketa JIROUSKOVA et al.

Základní údaje

Originální název

Measurement of Liver Stiffness using Atomic Force Microscopy Coupled with Polarization Microscopy

Autoři

OJHA, Srikant; Jan PŘIBYL ; Šimon KLIMOVIČ; Daniel HADRABA; Marketa JIROUSKOVA a Martin GREGOR

Vydání

Journal of Visualized Experiments, JoVE, 2022, 1940-087X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.200

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/22:00126479

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

MAGNETIC-RESONANCE ELASTOGRAPHY; MR ELASTOGRAPHY; MECHANICAL-PROPERTIES; RAT-LIVER; FIBROSIS; INDENTATION; HOMEOSTASIS; DIAGNOSIS; MODEL

Štítky

14313050, CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 10. 2024 11:38, Mgr. Adéla Pešková

Anotace

V originále

Matrix stiffening has been recognized as one of the key drivers of the progression of liver fibrosis. It has profound effects on various aspects of cell behavior such as cell function, differentiation, and motility. However, as these processes are not homogeneous throughout the whole organ, it has become increasingly important to understand changes in the mechanical properties of tissues on the cellular level. To be able to monitor the stiffening of collagen-rich areas within the liver lobes, this paper presents a protocol for measuring liver tissue elastic moduli with high spatial precision by atomic force microscopy (AFM). AFM is a sensitive method with the potential to characterize local mechanical properties, calculated as Young's (also referred to as elastic) modulus. AFM coupled with polarization microscopy can be used to specifically locate the areas of fibrosis development based on the birefringence of collagen fibers in tissues. Using the presented protocol, we characterized the stiffness of collagen-rich areas from fibrotic mouse livers and corresponding areas in the livers of control mice. A prominent increase in the stiffness of collagen-positive areas was observed with fibrosis development. The presented protocol allows for a highly reproducible method of AFM measurement, due to the use of mildly fixed liver tissue, that can be used to further the understanding of disease-initiated changes in local tissue mechanical properties and their effect on the fate of neighboring cells.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

EF18_046/0016045, projekt VaV

Název: Modernizace národní infrastruktury pro biologické a medicínské zobrazování Czech-BioImaging

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LX22NPO5102, projekt VaV

Název: Národní ústav pro výzkum rakoviny (Akronym: NÚVR)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro výzkum rakoviny, 5.1 EXCELES

Přiložené soubory

Measurement_of_Liver_Stiffness_Using_Atomic_Force_Microscopy_Coupled_with_Polarization_Microscopy.pdf

Citovat

OJHA, Srikant; Jan PŘIBYL; Šimon KLIMOVIČ; Daniel HADRABA; Marketa JIROUSKOVA a Martin GREGOR. Measurement of Liver Stiffness using Atomic Force Microscopy Coupled with Polarization Microscopy. Journal of Visualized Experiments. JoVE, 2022, roč. 2022, č. 185, s. 1-21. ISSN 1940-087X. Dostupné z: https://doi.org/10.3791/63974.

@article{2212607,
   author = {Ojha, Srikant and Přibyl, Jan and Klimovič, Šimon and Hadraba, Daniel and Jirouskova, Marketa and Gregor, Martin},
   article_number = {185},
   doi = {https://doi.org/10.3791/63974},
   keywords = {MAGNETIC-RESONANCE ELASTOGRAPHY; MR ELASTOGRAPHY; MECHANICAL-PROPERTIES; RAT-LIVER; FIBROSIS; INDENTATION; HOMEOSTASIS; DIAGNOSIS; MODEL},
   language = {eng},
   issn = {1940-087X},
   journal = {Journal of Visualized Experiments},
   title = {Measurement of Liver Stiffness using Atomic Force Microscopy Coupled with Polarization Microscopy},
   url = {https://www.jove.com/t/63974/measurement-liver-stiffness-using-atomic-force-microscopy-coupled},
   volume = {2022},
   year = {2022}
}

TY  - JOUR
ID  - 2212607
AU  - Ojha, Srikant - Přibyl, Jan - Klimovič, Šimon - Hadraba, Daniel - Jirouskova, Marketa - Gregor, Martin
PY  - 2022
TI  - Measurement of Liver Stiffness using Atomic Force Microscopy Coupled with Polarization Microscopy
JF  - Journal of Visualized Experiments
VL  - 2022
IS  - 185
SP  - 1-21
EP  - 1-21
PB  - JoVE
SN  - 1940087X
KW  - MAGNETIC-RESONANCE ELASTOGRAPHY
KW  - MR ELASTOGRAPHY
KW  - MECHANICAL-PROPERTIES
KW  - RAT-LIVER
KW  - FIBROSIS
KW  - INDENTATION
KW  - HOMEOSTASIS
KW  - DIAGNOSIS
KW  - MODEL
UR  - https://www.jove.com/t/63974/measurement-liver-stiffness-using-atomic-force-microscopy-coupled
N2  - Matrix stiffening has been recognized as one of the key drivers of the progression of liver fibrosis. It has profound effects on various aspects of cell behavior such as cell function, differentiation, and motility. However, as these processes are not homogeneous throughout the whole organ, it has become increasingly important to understand changes in the mechanical properties of tissues on the cellular level. To be able to monitor the stiffening of collagen-rich areas within the liver lobes, this paper presents a protocol for measuring liver tissue elastic moduli with high spatial precision by atomic force microscopy (AFM). AFM is a sensitive method with the potential to characterize local mechanical properties, calculated as Young's (also referred to as elastic) modulus. AFM coupled with polarization microscopy can be used to specifically locate the areas of fibrosis development based on the birefringence of collagen fibers in tissues. Using the presented protocol, we characterized the stiffness of collagen-rich areas from fibrotic mouse livers and corresponding areas in the livers of control mice. A prominent increase in the stiffness of collagen-positive areas was observed with fibrosis development. The presented protocol allows for a highly reproducible method of AFM measurement, due to the use of mildly fixed liver tissue, that can be used to further the understanding of disease-initiated changes in local tissue mechanical properties and their effect on the fate of neighboring cells.
ER  -

OJHA, Srikant; Jan PŘIBYL; Šimon KLIMOVIČ; Daniel HADRABA; Marketa JIROUSKOVA a Martin GREGOR. Measurement of Liver Stiffness using Atomic Force Microscopy Coupled with Polarization Microscopy. \textit{Journal of Visualized Experiments}. JoVE, 2022, roč.~2022, č.~185, s.~1-21. ISSN~1940-087X. Dostupné z: https://doi.org/10.3791/63974.

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