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@article{2212611,
author = {Urazbakhtin, Shamil and Smirnova, Anastasia and Volakhava, Anastasiya and Zerkalenkova, Elena and Salyutina, Maria and Doubek, Michael and Jelinkova, Hana and Khudainazarova, Nelly and Volchkov, Egor and Belyaeva, Laima and Komech, Ekaterina and Pavlová, Šárka and Lebedev, Yuri and Plevová, Karla and Olshanskaya, Yulia and Komkov, Alexander and Mamedov, Ilgar},
article_location = {Basel},
article_number = {3},
doi = {http://dx.doi.org/10.3390/ijms23031756},
keywords = {acute leukemia; retroelements; mobile elements; tumor-specific insertions},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {The Absence of Retroelement Activity Is Characteristic for Childhood Acute Leukemias and Adult Acute Lymphoblastic Leukemia},
url = {https://www.mdpi.com/1422-0067/23/3/1756},
volume = {23},
year = {2022}
}
TY - JOUR
ID - 2212611
AU - Urazbakhtin, Shamil - Smirnova, Anastasia - Volakhava, Anastasiya - Zerkalenkova, Elena - Salyutina, Maria - Doubek, Michael - Jelinkova, Hana - Khudainazarova, Nelly - Volchkov, Egor - Belyaeva, Laima - Komech, Ekaterina - Pavlová, Šárka - Lebedev, Yuri - Plevová, Karla - Olshanskaya, Yulia - Komkov, Alexander - Mamedov, Ilgar
PY - 2022
TI - The Absence of Retroelement Activity Is Characteristic for Childhood Acute Leukemias and Adult Acute Lymphoblastic Leukemia
JF - International Journal of Molecular Sciences
VL - 23
IS - 3
SP - 1756
EP - 1756
PB - Multidisciplinary Digital Publishing Institute
SN - 14220067
KW - acute leukemia
KW - retroelements
KW - mobile elements
KW - tumor-specific insertions
UR - https://www.mdpi.com/1422-0067/23/3/1756
N2 - Retroelements (RE) have been proposed as important players in cancerogenesis. Different cancer types are characterized by a different level of tumor-specific RE insertions. In previous studies, small cohorts of hematological malignancies, such as acute myeloid leukemia, multiple myeloma, and chronic lymphocytic leukemia have been characterized by a low level of RE insertional activity. Acute lymphoblastic leukemia (ALL) in adults and childhood acute leukemias have not been studied in this context. We performed a search for new RE insertions (Alu and L1) in 44 childhood ALL, 14 childhood acute myeloid leukemia, and 14 adult ALL samples using a highly sensitive NGS-based approach. First, we evaluated the method sensitivity revealing the 1% detection threshold for the proportion of cells with specific RE insertion. Following this result, we did not identify new tumor-specific RE insertions in the tested cohort of acute leukemia samples at the established level of sensitivity. Additionally, we analyzed the transcription levels of active L1 copies and found them increased. Thus, the increased transcription of active L1 copies is not sufficient for overt elevation of L1 retrotranspositional activity in leukemia.
ER -
URAZBAKHTIN, Shamil, Anastasia SMIRNOVA, Anastasiya VOLAKHAVA, Elena ZERKALENKOVA, Maria SALYUTINA, Michael DOUBEK, Hana JELINKOVA, Nelly KHUDAINAZAROVA, Egor VOLCHKOV, Laima BELYAEVA, Ekaterina KOMECH, Šárka PAVLOVÁ, Yuri LEBEDEV, Karla PLEVOVÁ, Yulia OLSHANSKAYA, Alexander KOMKOV a Ilgar MAMEDOV. The Absence of Retroelement Activity Is Characteristic for Childhood Acute Leukemias and Adult Acute Lymphoblastic Leukemia. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2022, roč.~23, č.~3, s.~1756-1768. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms23031756.
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