In situ cryo-electron tomography of enterovirus cell entry

a 2022

In situ cryo-electron tomography of enterovirus cell entry

TREBICHALSKÁ, Zuzana, Liya MUKHAMEDOVA, Aygul ISHEMGULOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK et. al.

Základní údaje

Originální název

In situ cryo-electron tomography of enterovirus cell entry

Autoři

TREBICHALSKÁ, Zuzana, Liya MUKHAMEDOVA, Aygul ISHEMGULOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK, Jiří NOVÁČEK a Pavel PLEVKA

Vydání

XVIII Discussions in Structural Molecular Biology and 5th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology), 2022

Další údaje

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Středoevropský technologický institut

ISSN

Příznaky

Mezinárodní význam

Změněno: 31. 8. 2022 09:23, Mgr. Zuzana Trebichalská

Anotace

V originále

Enteroviruses from the family Picornaviridae are human pathogens that cause a range of diseases from the common cold to severe brain inflammation. Despite the societal and economic impact of enteroviruses, the available treatments are only symptomatic. The enterovirus cell entry and the release of the viruses from endosomes are potential targets for antiviral therapeutics. However, the details of these phenomena are not well understood. Here, we used in situ cryo-electron tomography to visualize the cell entry and genome release of human rhinovirus 2. We observed endosome membrane remodelling and breakage followed by virus escape into the cytoplasm. We demonstrate that the endosome disruption is mediated by overactivation of a cellular mechanism by showing that endocytosis of very-low-density lipoprotein, the natural substrate of rhinovirus 2 receptor, also results in endosome disruption. The described mechanism of rhinovirus 2 cell entry is supported by data collected on other enteroviruses. Our results give evidence of the cellular mechanisms these viruses employ to enter cell hosts. We acknowledge the Cryo-electron microscopy and Tomography Core Facility of CEITEC MU for their support in data collection and analysis.

Návaznosti

GX19-25982X, projekt VaV

Název: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Grantová agentura ČR, Structural study of enterovirus replication in situ

Citovat

TREBICHALSKÁ, Zuzana, Liya MUKHAMEDOVA, Aygul ISHEMGULOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK, Jiří NOVÁČEK a Pavel PLEVKA. In situ cryo-electron tomography of enterovirus cell entry. In XVIII Discussions in Structural Molecular Biology and 5th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology). 2022. ISSN 1805-4382.

@proceedings{2214279,
   author = {Trebichalská, Zuzana and Mukhamedova, Liya and Ishemgulova, Aygul and Šmerdová, Lenka and Füzik, Tibor and Nováček, Jiří and Plevka, Pavel},
   booktitle = {XVIII Discussions in Structural Molecular Biology and 5th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology)},
   title = {In situ cryo-electron tomography of enterovirus cell entry},
   year = {2022}
}

TY  - CONF
ID  - 2214279
AU  - Trebichalská, Zuzana - Mukhamedova, Liya - Ishemgulova, Aygul - Šmerdová, Lenka - Füzik, Tibor - Nováček, Jiří - Plevka, Pavel
PY  - 2022
TI  - In situ cryo-electron tomography of enterovirus cell entry
N2  - Enteroviruses from the family Picornaviridae are human pathogens that cause a range of diseases from the common cold to severe brain inflammation. Despite the societal and economic impact of enteroviruses, the available treatments are only symptomatic. The enterovirus cell entry and the release of the viruses from endosomes are potential targets for antiviral therapeutics. However, the details of these phenomena are not well understood. Here, we used in situ cryo-electron tomography to visualize the cell entry and genome release of human rhinovirus 2. We observed endosome membrane remodelling and breakage followed by virus escape into the cytoplasm. We demonstrate that the endosome disruption is mediated by overactivation of a cellular mechanism by showing that endocytosis of very-low-density lipoprotein, the natural substrate of rhinovirus 2 receptor, also results in endosome disruption. The described mechanism of rhinovirus 2 cell entry is supported by data collected on other enteroviruses. Our results give evidence of the cellular mechanisms these viruses employ to enter cell hosts. We acknowledge the Cryo-electron microscopy and Tomography Core Facility of CEITEC MU for their support in data collection and analysis.
ER  -

TREBICHALSKÁ, Zuzana, Liya MUKHAMEDOVA, Aygul ISHEMGULOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK, Jiří NOVÁČEK a Pavel PLEVKA. In situ cryo-electron tomography of enterovirus cell entry. In \textit{XVIII Discussions in Structural Molecular Biology and 5th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology)}. 2022. ISSN~1805-4382.

Podrobný výpis o publikaci