Elucidating the life cycle of human enteroviruses in situ

a 2021

Elucidating the life cycle of human enteroviruses in situ

TREBICHALSKÁ, Zuzana, Jiří NOVÁČEK a Pavel PLEVKA

Základní údaje

Originální název

Elucidating the life cycle of human enteroviruses in situ

Autoři

TREBICHALSKÁ, Zuzana, Jiří NOVÁČEK a Pavel PLEVKA

Vydání

1st Student Conference in Structural Biology, 2021

Další údaje

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

Příznaky

Mezinárodní význam

Změněno: 4. 9. 2022 08:50, Mgr. Zuzana Trebichalská

Anotace

V originále

Human echovirus 18 is a member of the Enterovirus B species. It has diverse tissue tropism: the cells of the nervous system, lower respiratory tract, and gastrointestinal system. The long-term effects of viral encephalitis are acquired brain injury, memory loss, and altered consciousness [1]. Furthermore, it has been reported that this virus is connected to chronic gastrointestinal diseases, particularly, to Crohn’s disease [2]. In this project, we characterize the interactions of cell and Echovirus 18 particles and the genome delivery in situ. We study the replication cycle to determine the mechanisms of recombination that cause the emergence of new viral strains. By observation of the virion assembly, we identify the mechanism of genome packaging. We use the focused-ion beam milling (FIBM) to prepare lamellae of the infected cells and image them using cryo-electron microscopy. The thin (200 to 300 nm) lamellae are used to record tomographic tilt series [3, 4]. The image alignment is followed by the 3D reconstruction of cell sections [5]. The reconstructed parts of cells provide information about the positioning and interactions of the macromolecular complexes of the cell with the virion assemblies and their intermediate states. Since enteroviruses are a homogeneous group of viruses, the data for human enterovirus 18 will be relevant also as a reference for further experiments on other enterovirus species. 1. M. C. Huang, S. M. Wang, Y. W. Hsu, H. C. Lin, C. Y. Chi, C. C. Liu, Pediatrics, 118, (2006), e1785. 2. J. Chia, A. Chia, D. Wang, R. El-Habbal, S. Jyotheeswaran, E. McCloud, Open J Gastroenterol, 9, (2019) 174. 3. A. Rigort, F. J. Bäuerlein, E. Villa, M. Eibauer, T. Laugks, W. Baumeister, J. M. Plitzko, Proc Natl Acad Sci U.S.A., 109, (2012), 4449. 4. M. Schaffer, J. Mahamid, B. D. Engel, T. Laugks, W. Baumeister, J. M. Plitzko, J Struct Biol, 197 (2017), 73. 5. J. M. Plitzko, B. Schuler, P. Selenko, Curr Opin Struc Biol, 46, (2017), 110. We acknowledge the Cryo-electron microscopy and Tomography Core Facility of CEITEC MU for their support in data collection and analysis.

Návaznosti

GX19-25982X, projekt VaV

Název: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Grantová agentura ČR, Structural study of enterovirus replication in situ

Citovat

TREBICHALSKÁ, Zuzana, Jiří NOVÁČEK a Pavel PLEVKA. Elucidating the life cycle of human enteroviruses in situ. In 1st Student Conference in Structural Biology. 2021.

@proceedings{2215017,
   author = {Trebichalská, Zuzana and Nováček, Jiří and Plevka, Pavel},
   booktitle = {1st Student Conference in Structural Biology},
   title = {Elucidating the life cycle of human enteroviruses in situ},
   url = {http://cssb.structbio.org/1st-student-conference-in-structural-biology/},
   year = {2021}
}

TY  - CONF
ID  - 2215017
AU  - Trebichalská, Zuzana - Nováček, Jiří - Plevka, Pavel
PY  - 2021
TI  - Elucidating the life cycle of human enteroviruses in situ
UR  - http://cssb.structbio.org/1st-student-conference-in-structural-biology/
N2  - Human echovirus 18 is a member of the Enterovirus B species. It has diverse tissue tropism: the cells of the nervous system, lower respiratory tract, and gastrointestinal system. The long-term effects of viral encephalitis are acquired brain injury, memory loss, and altered consciousness [1]. Furthermore, it has been reported that this virus is connected to chronic gastrointestinal diseases, particularly, to Crohn’s disease [2]. In this project, we characterize the interactions of cell and Echovirus 18 particles and the genome delivery in situ. We study the replication cycle to determine the mechanisms of recombination that cause the emergence of new viral strains. By observation of the virion assembly, we identify the mechanism of genome packaging. We use the focused-ion beam milling (FIBM) to prepare lamellae of the infected cells and image them using cryo-electron microscopy. The thin (200 to 300 nm) lamellae are used to record tomographic tilt series [3, 4]. The image alignment is followed by the 3D reconstruction of cell sections [5]. The reconstructed parts of cells provide information about the positioning and interactions of the macromolecular complexes of the cell with the virion assemblies and their intermediate states. Since enteroviruses are a homogeneous group of viruses, the data for human enterovirus 18 will be relevant also as a reference for further experiments on other enterovirus species. 1. M. C. Huang, S. M. Wang, Y. W. Hsu, H. C. Lin, C. Y. Chi, C. C. Liu, Pediatrics, 118, (2006), e1785. 2. J. Chia, A. Chia, D. Wang, R. El-Habbal, S. Jyotheeswaran, E. McCloud, Open J Gastroenterol, 9, (2019) 174. 3. A. Rigort, F. J. Bäuerlein, E. Villa, M. Eibauer, T. Laugks, W. Baumeister, J. M. Plitzko, Proc Natl Acad Sci U.S.A., 109, (2012), 4449. 4. M. Schaffer, J. Mahamid, B. D. Engel, T. Laugks, W. Baumeister, J. M. Plitzko, J Struct Biol, 197 (2017), 73. 5. J. M. Plitzko, B. Schuler, P. Selenko, Curr Opin Struc Biol, 46, (2017), 110. We acknowledge the Cryo-electron microscopy and Tomography Core Facility of CEITEC MU for their support in data collection and analysis.
ER  -

TREBICHALSKÁ, Zuzana, Jiří NOVÁČEK a Pavel PLEVKA. Elucidating the life cycle of human enteroviruses in situ. In \textit{1st Student Conference in Structural Biology}. 2021.

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