Enteroviruses reach cytoplasm by endosome disruption

a 2022

Enteroviruses reach cytoplasm by endosome disruption

TREBICHALSKÁ, Zuzana, Aygul ISHEMGULOVA, Liya MUKHAMEDOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK et. al.

Základní údaje

Originální název

Enteroviruses reach cytoplasm by endosome disruption

Autoři

TREBICHALSKÁ, Zuzana, Aygul ISHEMGULOVA, Liya MUKHAMEDOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK, Jiří NOVÁČEK a Pavel PLEVKA

Vydání

5th Austrian Cryo-EM Symposium, 2022

Další údaje

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

Příznaky

Mezinárodní význam

Změněno: 12. 9. 2022 13:55, Mgr. Zuzana Trebichalská

Anotace

V originále

Enteroviruses, a large group of non-enveloped picornaviruses, are human pathogens causing a range of diseases, from the common cold to poliomyelitis. To initiate infecti- on, enteroviruses enter cells by receptor-mediated endocytosis. However, the details of enterovirus genome delivery are not well understood. In this project, we used cryo-electron tomography of thin parts of cells grown on electron microscopy grids to characterize the interactions of the cell and enterovirus particles in situ. The reconstructed areas of cells provide information about the endo- some membrane remodeling and disruption, followed by virus release into the cyto- plasm. We demonstrate that the endosome disruption is mediated by overactivation of a cellular mechanism by showing that endocytosis of very-low-density lipoprotein, the natural substrate of rhinovirus 2 receptor, also results in endosome disruption. The described mechanism of human rhinovirus 2 cell entry is supported by data col- lected on other enteroviruses. Our results give overview of the cellular mechanisms that enteroviruses utilize to en- ter cell hosts. The enterovirus cell entry and the release of the viruses from endoso- mes are potential targets for antiviral therapeutics.

Návaznosti

GX19-25982X, projekt VaV

Název: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Grantová agentura ČR, Structural study of enterovirus replication in situ

Citovat

TREBICHALSKÁ, Zuzana, Aygul ISHEMGULOVA, Liya MUKHAMEDOVA, Lenka ŠMERDOVÁ, Tibor FÜZIK, Jiří NOVÁČEK a Pavel PLEVKA. Enteroviruses reach cytoplasm by endosome disruption. In 5th Austrian Cryo-EM Symposium. 2022.

@proceedings{2217079,
   author = {Trebichalská, Zuzana and Ishemgulova, Aygul and Mukhamedova, Liya and Šmerdová, Lenka and Füzik, Tibor and Nováček, Jiří and Plevka, Pavel},
   booktitle = {5th Austrian Cryo-EM Symposium},
   title = {Enteroviruses reach cytoplasm by endosome disruption},
   url = {https://www.imp.ac.at/research/cryo-em-symposium/},
   year = {2022}
}

TY  - CONF
ID  - 2217079
AU  - Trebichalská, Zuzana - Ishemgulova, Aygul - Mukhamedova, Liya - Šmerdová, Lenka - Füzik, Tibor - Nováček, Jiří - Plevka, Pavel
PY  - 2022
TI  - Enteroviruses reach cytoplasm by endosome disruption
UR  - https://www.imp.ac.at/research/cryo-em-symposium/
N2  - Enteroviruses, a large group of non-enveloped picornaviruses, are human pathogens causing a range of diseases, from the common cold to poliomyelitis. To initiate infecti- on, enteroviruses enter cells by receptor-mediated endocytosis. However, the details of enterovirus genome delivery are not well understood. In this project, we used cryo-electron tomography of thin parts of cells grown on electron microscopy grids to characterize the interactions of the cell and enterovirus particles in situ. The reconstructed areas of cells provide information about the endo- some membrane remodeling and disruption, followed by virus release into the cyto- plasm. We demonstrate that the endosome disruption is mediated by overactivation of a cellular mechanism by showing that endocytosis of very-low-density lipoprotein, the natural substrate of rhinovirus 2 receptor, also results in endosome disruption. The described mechanism of human rhinovirus 2 cell entry is supported by data col- lected on other enteroviruses. Our results give overview of the cellular mechanisms that enteroviruses utilize to en- ter cell hosts. The enterovirus cell entry and the release of the viruses from endoso- mes are potential targets for antiviral therapeutics.
ER  -

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