Enhancing the sensitivity of laser-induced breakdown
spectroscopy for the detection of nanoparticle-labeled
cancerous tissues

a 2022

Enhancing the sensitivity of laser-induced breakdown spectroscopy for the detection of nanoparticle-labeled cancerous tissues

KAISER, Jozef, Karolína VYTISKOVÁ, Karel NOVOTNÝ, Zdeněk FARKA, Petr SKLÁDAL et. al.

Základní údaje

Originální název

Enhancing the sensitivity of laser-induced breakdown spectroscopy for the detection of nanoparticle-labeled cancerous tissues

Autoři

KAISER, Jozef, Karolína VYTISKOVÁ, Karel NOVOTNÝ, Zdeněk FARKA, Petr SKLÁDAL a Pavel POŘÍZKA

Vydání

European Symposium on Analytical Spectrometry (ESAS) 2022, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-88195-41-2

Příznaky

Mezinárodní význam

Změněno: 18. 9. 2022 12:07, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Fluorescent labels, based on conjugates of antibodies with various nanoparticles, are popular because of their broad detection posibilities (plate readers, microscopes). However, fluorescence is a limiting factor in e.g. multiplexing; only a limited number of dyes can be detected due to the overlaps of dye spectra or optical filters. Thus, novel readout methods are necessary to overcome such limitations. Laser spectroscopy – namely Laser-Induced Breakdown Spectroscopy (LIBS) – is a prospective option in nanoparticle detection. LIBS provides superior performance in terms of throughput and repetition rate enabling large-scale elemental imaging in combination with high lateral resolution. In this talk, we will demonstrate the feasibility of LIBS as a readout method in immunochemical assays and imaging of cancer tissues (breast cancer) through the indirect detection of upconversion nanoparticles (UCNPs). The LIBS scanning enabled detecting the characteristic elemental signature of yttrium as a principal constituent of UCNP, thus indirectly providing a reliable way to differentiate between HER2-positive BT-474 cells and HER2-negative MDA-MB-231 cells. The comparison of results with upconversion optical microscopy and luminescence intensity scanning confirmed that LIBS is a promising alternative for the readout of immunohistochemical samples. Moreover, we have deployed the collinear double-pulse arrangement for enhanced sensitivity and detection capability.

Návaznosti

GA22-27580S, projekt VaV

Název: Laserová spektroskopie v imunostanovení a zobrazování s nanometalickými značkami (Akronym: IMMUNOLIBS)

Investor: Grantová agentura ČR, Laserová spektroskopie v imunostanovení a zobrazování s nanometalickými značkami

Citovat

KAISER, Jozef, Karolína VYTISKOVÁ, Karel NOVOTNÝ, Zdeněk FARKA, Petr SKLÁDAL a Pavel POŘÍZKA. Enhancing the sensitivity of laser-induced breakdown spectroscopy for the detection of nanoparticle-labeled cancerous tissues. In European Symposium on Analytical Spectrometry (ESAS) 2022. 2022. ISBN 978-80-88195-41-2.

@proceedings{2218278,
   author = {Kaiser, Jozef and Vytisková, Karolína and Novotný, Karel and Farka, Zdeněk and Skládal, Petr and Pořízka, Pavel},
   booktitle = {European Symposium on Analytical Spectrometry (ESAS) 2022},
   language = {eng},
   isbn = {978-80-88195-41-2},
   title = {Enhancing the sensitivity of laser-induced breakdown spectroscopy for the detection of nanoparticle-labeled cancerous tissues},
   url = {http://esas-cssc2022.spektroskopie.cz/files/ESAS%202022%20BOOK%20OF%20ABSTRACTS.pdf},
   year = {2022}
}

TY  - CONF
ID  - 2218278
AU  - Kaiser, Jozef - Vytisková, Karolína - Novotný, Karel - Farka, Zdeněk - Skládal, Petr - Pořízka, Pavel
PY  - 2022
TI  - Enhancing the sensitivity of laser-induced breakdown spectroscopy for the detection of nanoparticle-labeled cancerous tissues
SN  - 9788088195412
UR  - http://esas-cssc2022.spektroskopie.cz/files/ESAS%202022%20BOOK%20OF%20ABSTRACTS.pdf
N2  - Fluorescent labels, based on conjugates of antibodies with various nanoparticles, are popular because of their broad detection posibilities (plate readers, microscopes). However, fluorescence is a limiting factor in e.g. multiplexing; only a limited number of dyes can be detected due to the overlaps of dye spectra or optical filters. Thus, novel readout methods are necessary to overcome such limitations. Laser spectroscopy – namely Laser-Induced Breakdown Spectroscopy (LIBS) – is a prospective option in nanoparticle detection. LIBS provides superior performance in terms of throughput and repetition rate enabling large-scale elemental imaging in combination with high lateral resolution. In this talk, we will demonstrate the feasibility of LIBS as a readout method in immunochemical assays and imaging of cancer tissues (breast cancer) through the indirect detection of upconversion nanoparticles (UCNPs). The LIBS scanning enabled detecting the characteristic elemental signature of yttrium as a principal constituent of UCNP, thus indirectly providing a reliable way to differentiate between HER2-positive BT-474 cells and HER2-negative MDA-MB-231 cells. The comparison of results with upconversion optical microscopy and luminescence intensity scanning confirmed that LIBS is a promising alternative for the readout of immunohistochemical samples. Moreover, we have deployed the collinear double-pulse arrangement for enhanced sensitivity and detection capability.
ER  -

KAISER, Jozef, Karolína VYTISKOVÁ, Karel NOVOTNÝ, Zdeněk FARKA, Petr SKLÁDAL a Pavel POŘÍZKA. Enhancing the sensitivity of laser-induced breakdown spectroscopy for the detection of nanoparticle-labeled cancerous tissues. In \textit{European Symposium on Analytical Spectrometry (ESAS) 2022}. 2022. ISBN~978-80-88195-41-2.

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