Selected ginsenosides interfere efficiently with hepatitis B
virus mRNA expression levels and suppress viral surface antigen
secretion.

J 2022

Selected ginsenosides interfere efficiently with hepatitis B virus mRNA expression levels and suppress viral surface antigen secretion.

DURAISAMY, Ganesh S., Eunji JO, Ivana HUVAROVÁ, Kyu-Ho P. PARK, Zbyněk HEGER et. al.

Základní údaje

Originální název

Selected ginsenosides interfere efficiently with hepatitis B virus mRNA expression levels and suppress viral surface antigen secretion.

Autoři

DURAISAMY, Ganesh S., Eunji JO, Ivana HUVAROVÁ, Kyu-Ho P. PARK, Zbyněk HEGER, Vojtěch ADAM, Daniel RŮŽEK (203 Česká republika, domácí), Marc P. WINDISCH a Andrew D. MILLER (garant)

Vydání

Heliyon, Elsevier, 2022, 2405-8440

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.000

Kód RIV

RIV/00216224:14310/22:00126712

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.heliyon.2022.e10465

UT WoS

000862260600017

Klíčová slova anglicky

Ginsenoside; Hepatitis B virus; Lamivudine; Nucleoside/nucleotide analogues; Hepatitis B surface protein; Hepatitis B surface antigen; Chronic hepatitis B virus; Drug combinations

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 1. 2023 14:36, Mgr. Marie Šípková, DiS.

Anotace

V originále

Ginsenosides are a class of natural steroid glycosides and triterpene saponins found in Panax ginseng. After screening of a commercial ginsenoside compound library for low cellular cytotoxicity and the ability to mediate efficient reductions in hepatitis B virus (HBV) mRNA expression levels in HepG2.2.15 cells, three ginsenosides (Rg6, Rh4, and Rb3) are selected. Thereafter, using the same cellular model, all three ginsenosides are shown to mediate efficient, selective inhibition of HBV mRNA expression levels, and also interfere with the secretion of both HBV particles and hepatitis B surface antigen (HBsAg). Drug combination studies are performed in both HepG2.2.15 and HBV-infected HepG2-NTCPsec+ cell models with the selected ginsenosides and lamivudine (LMV), a nucleoside analogue used to treat chronic hepatitis B (CHB) infections. These studies, involving RT-qPCR and ELISA, suggest that Rh4/LMV combinations in particular act synergistically to inhibit the secretion of HBV particles and HBsAg. Therefore, on the assumption that appropriate in vivo data are in future agreement, Rh4, in particular, might be used in combination with nucleoside/nucleotide analogues (NUCs) to devise an effective, cost-efficient combination therapy for the treatment of patients with CHB infections.

Citovat

DURAISAMY, Ganesh S., Eunji JO, Ivana HUVAROVÁ, Kyu-Ho P. PARK, Zbyněk HEGER, Vojtěch ADAM, Daniel RŮŽEK, Marc P. WINDISCH a Andrew D. MILLER. Selected ginsenosides interfere efficiently with hepatitis B virus mRNA expression levels and suppress viral surface antigen secretion. Heliyon. Elsevier, 2022, roč. 8, č. 9, s. 1-13. ISSN 2405-8440. Dostupné z: https://dx.doi.org/10.1016/j.heliyon.2022.e10465.

@article{2219059,
   author = {Duraisamy, Ganesh S. and Jo, Eunji and Huvarová, Ivana and Park, KyuandHo P. and Heger, Zbyněk and Adam, Vojtěch and Růžek, Daniel and Windisch, Marc P. and Miller, Andrew D.},
   article_number = {9},
   doi = {http://dx.doi.org/10.1016/j.heliyon.2022.e10465},
   keywords = {Ginsenoside; Hepatitis B virus; Lamivudine; Nucleoside/nucleotide analogues; Hepatitis B surface protein; Hepatitis B surface antigen; Chronic hepatitis B virus; Drug combinations},
   language = {eng},
   issn = {2405-8440},
   journal = {Heliyon},
   title = {Selected ginsenosides interfere efficiently with hepatitis B virus mRNA expression levels and suppress viral surface antigen secretion.},
   url = {https://doi.org/10.1016/j.heliyon.2022.e10465},
   volume = {8},
   year = {2022}
}

TY  - JOUR
ID  - 2219059
AU  - Duraisamy, Ganesh S. - Jo, Eunji - Huvarová, Ivana - Park, Kyu-Ho P. - Heger, Zbyněk - Adam, Vojtěch - Růžek, Daniel - Windisch, Marc P. - Miller, Andrew D.
PY  - 2022
TI  - Selected ginsenosides interfere efficiently with hepatitis B virus mRNA expression levels and suppress viral surface antigen secretion.
JF  - Heliyon
VL  - 8
IS  - 9
SP  - 1-13
EP  - 1-13
PB  - Elsevier
SN  - 24058440
KW  - Ginsenoside
KW  - Hepatitis B virus
KW  - Lamivudine
KW  - Nucleoside/nucleotide analogues
KW  - Hepatitis B surface protein
KW  - Hepatitis B surface antigen
KW  - Chronic hepatitis B virus
KW  - Drug combinations
UR  - https://doi.org/10.1016/j.heliyon.2022.e10465
N2  - Ginsenosides are a class of natural steroid glycosides and triterpene saponins found in Panax ginseng. After screening of a commercial ginsenoside compound library for low cellular cytotoxicity and the ability to mediate efficient reductions in hepatitis B virus (HBV) mRNA expression levels in HepG2.2.15 cells, three ginsenosides (Rg6, Rh4, and Rb3) are selected. Thereafter, using the same cellular model, all three ginsenosides are shown to mediate efficient, selective inhibition of HBV mRNA expression levels, and also interfere with the secretion of both HBV particles and hepatitis B surface antigen (HBsAg). Drug combination studies are performed in both HepG2.2.15 and HBV-infected HepG2-NTCPsec+ cell models with the selected ginsenosides and lamivudine (LMV), a nucleoside analogue used to treat chronic hepatitis B (CHB) infections. These studies, involving RT-qPCR and ELISA, suggest that Rh4/LMV combinations in particular act synergistically to inhibit the secretion of HBV particles and HBsAg. Therefore, on the assumption that appropriate in vivo data are in future agreement, Rh4, in particular, might be used in combination with nucleoside/nucleotide analogues (NUCs) to devise an effective, cost-efficient combination therapy for the treatment of patients with CHB infections.
ER  -

DURAISAMY, Ganesh S., Eunji JO, Ivana HUVAROVÁ, Kyu-Ho P. PARK, Zbyněk HEGER, Vojtěch ADAM, Daniel RŮŽEK, Marc P. WINDISCH a Andrew D. MILLER. Selected ginsenosides interfere efficiently with hepatitis B virus mRNA expression levels and suppress viral surface antigen secretion. \textit{Heliyon}. Elsevier, 2022, roč.~8, č.~9, s.~1-13. ISSN~2405-8440. Dostupné z: https://dx.doi.org/10.1016/j.heliyon.2022.e10465.

Podrobný výpis o publikaci