J 2022

Harmonized Quality Assurance/Quality Control Provisions for Nontargeted Measurement of Urinary Pesticide Biomarkers in the HBM4EU Multisite SPECIMEn Study

VITALE, Chiara Maria, Arjen LOMMEN, Carolin HUBER, Kevin WAGNER, Garlito Molina BORJA et. al.

Základní údaje

Originální název

Harmonized Quality Assurance/Quality Control Provisions for Nontargeted Measurement of Urinary Pesticide Biomarkers in the HBM4EU Multisite SPECIMEn Study

Autoři

VITALE, Chiara Maria (380 Itálie, domácí), Arjen LOMMEN, Carolin HUBER, Kevin WAGNER, Garlito Molina BORJA, Rosalie NIJSSEN, Elliott James PRICE (826 Velká Británie a Severní Irsko, domácí), Marco BLOKLAND, van Tricht FREDERIKE, Hans G J MOL, Martin KRAUSS, Laurent DEBRAUWER, Olga PARDO, Nuria LEON, Jana KLÁNOVÁ (203 Česká republika, domácí) a Jean-Philippe ANTIGNAC (garant)

Vydání

Analytical chemistry, American Chemical Society, 2022, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 7.400

Kód RIV

RIV/00216224:14310/22:00126753

Organizační jednotka

Přírodovědecká fakulta

DOI

UT WoS

000818957300001

Klíčová slova anglicky

Anatomy; Extraction; Mass spectrometry; Quality management; Solvents

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 6. 12. 2022 11:22, Mgr. Marie Šípková, DiS.

Anotace

V originále

A set of quality assurance/quality control (QA/QC) criteria for nontargeted measurement of pesticide exposure markers in a large-scale study of human urine has been proposed and applied across five laboratories within the HBM4EU project. Quality control material, including reference standards and fortified pooled urine samples (QC urine) were prepared in a centralized way and distributed across participants to monitor analytical performance and consistency of the liquid chromatography coupled to high-resolution mass spectrometry data generated with a harmonized workflow. Signal intensities, mass accuracy, and retention times of selected QA/QC markers covering a broad range of physicochemical properties were monitored across QC solvent standards, QC urine samples, study urine samples, and procedural blanks, setting acceptance thresholds for repeatability and accuracy. Overall, results showed high repeatability of the collected data. The RSDs of the signal intensities were typically below 20-30% in QC and study samples, with good stability of the chromatographic separation (retention time drift within 2-4 s intrabatch and 5 s interbatch) and excellent mass accuracy (average error < 2 ppm). The use of the proposed criteria allowed for the identification of handling errors, instrumental issues, and potential batch effects. This is the first elaboration of harmonized QA/QC criteria applied across multiple laboratories to assess the quality of data generated by nontargeted analysis of human samples.

Návaznosti

EF17_043/0009632, projekt VaV
Název: CETOCOEN Excellence
EF20_079/0017045, projekt VaV
Název: MSCAfellow4@MUNI
LM2018121, projekt VaV
Název: Výzkumná infrastruktura RECETOX (Akronym: RECETOX RI)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX RI
733032, interní kód MU
Název: European Human Biomonitoring Initiative (Akronym: HBM4EU)
Investor: Evropská unie, European Human Biomonitoring Initiative, Health, demographic change and wellbeing (Societal Challenges)
857560, interní kód MU
(Kód CEP: EF17_043/0009632)
Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)
Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation