2022
Validation of Medicinal Leeches (Hirudo medicinalis) as a Non-invasive Blood Sampling Tool for Hematology and Biochemistry Profiling in Mammals
KVAPIL, Pavel, Oldřich TOMÁŠEK, Eva BÁRTOVÁ, Mojca HAREJ, Marjan KASTELIC et. al.Základní údaje
Originální název
Validation of Medicinal Leeches (Hirudo medicinalis) as a Non-invasive Blood Sampling Tool for Hematology and Biochemistry Profiling in Mammals
Autoři
KVAPIL, Pavel (705 Slovinsko, garant), Oldřich TOMÁŠEK (203 Česká republika, domácí), Eva BÁRTOVÁ (203 Česká republika), Mojca HAREJ (705 Slovinsko), Marjan KASTELIC (705 Slovinsko), Tit PRIMOŽIČ (705 Slovinsko), Nikola KAŠPÁRKOVÁ (203 Česká republika) a Jožko RAČNIK (705 Slovinsko)
Vydání
Frontiers in Veterinary Science, Frontiers Media SA, 2022, 2297-1769
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10613 Zoology
Stát vydavatele
Švýcarsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 3.200
Kód RIV
RIV/00216224:14310/22:00129184
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000760800300001
Klíčová slova anglicky
hematology; biochemistry; non-invasive blood sampling; medicinal leech; Hirudo medicinalis
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 11. 7. 2023 09:54, Mgr. Marie Šípková, DiS.
Anotace
V originále
Blood sampling is a challenging procedure in many captive animals. Although manual restraint or anesthesia are usually possible, they entail intense stress and a high risk of injuries or organ failure. Blood sampling using medicinal leeches (Hirudo medicinalis) represents a promising non-invasive alternative to venipuncture; however, leech blood meal was to date used only for qualitative analyses such as genetic or serological screenings. Hence, the aim of this study was to evaluate the suitability of the leech blood sampling method for quantification of hematological and biochemical parameters. Medicinal leeches were manually applied on 67 zoo animals of eleven species, and control blood samples were obtained by venipuncture of the jugular vein. The leeches drew up to 20 ml of blood in 20 to 55 min. Although most hematological and biochemical parameters were significantly altered in leech-derived samples, their values showed strong (r = 0.62–0.79; 10/24 parameters) to very strong (r > 0.8; 13/24 parameters) correlations with venipuncture in all blood parameters, except for sodium (r = 0.39). As the parameter alterations and correlations were similar among species, simple cross-species regression formulas were sufficient to correct the alterations, thereby ensuring good repeatability between leeches and venipuncture in most parameters. Our data thus suggest that medicinal leeches can be used as a reliable non-invasive and stress-reducing alternative to standard venipuncture, even for quantitative assays. This opens new opportunities for a significant improvement to animal welfare in zoological gardens, conservation programmes, and ecophysiological research, where quantification of blood parameters is often needed.
Návaznosti
GA21-22160S, projekt VaV |
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