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@proceedings{2223865,
author = {Pinďáková, Eliška and Danihlík, Jiří and Dostálková, Silvie and Petřivalský, Marek and Dobeš, Pavel and Hurychová, Jana and Hyršl, Pavel},
booktitle = {EurBee 9 – 9th European Congress of Apidology},
keywords = {Oxidative stress; Lipid peroxidation; Varroa destructor},
language = {eng},
title = {Oxidative stress associated with varroa parasitism: evaluation of lipid peroxidation in infested bees},
year = {2022}
}
TY - CONF
ID - 2223865
AU - Pinďáková, Eliška - Danihlík, Jiří - Dostálková, Silvie - Petřivalský, Marek - Dobeš, Pavel - Hurychová, Jana - Hyršl, Pavel
PY - 2022
TI - Oxidative stress associated with varroa parasitism: evaluation of lipid peroxidation in infested bees
KW - Oxidative stress
KW - Lipid peroxidation
KW - Varroa destructor
N2 - Varroa destructor mites are responsible for the most severe problems with honey bee health worldwide. They cause a disease named varroosis. The mites transmit a wide array of highly lethal viruses to honey bee colonies, typically the deformed wing virus (DWV). Bee responses to external and internal stressors include the production of reactive oxygen species (ROS), which occurs as a part of humoral immunity. High ROS production or impaired functions of the antioxidant system result in ROS accumulation and oxidative stress, leading to damage to essential biomolecules such as lipids, proteins, and nucleic acids. Under disturbed redox balance, cellular lipids represent a primary target of ROSinduced oxidative modifications. The process of lipid peroxidation can be assessed either at the level of reactive primary products, lipid hydroperoxides (LOOH) or at the level of stable terminal products represented by malondialdehyde (MDA). This study aimed to determine lipid peroxidation levels in adult honey bees (Apis mellifera) after infestation by Varroa destructor mites during the pupal period. The collected bees were kept in plastic cages in a thermostat (34 ± 1 °C, 80 % relative humidity, dark) for 10 days and fed with a sucrose solution (1:1 w/v; sucrose/ water) and pollen ad libitum. Levels of LOOHs were quantified by the xylenol orange (FOX2) method, whereas MDA levels were determined by the thiobarbituric acid (TBARS) method. Bee infestation resulted in significantly decreased levels of both lipid peroxidation parameters compared to the control non-infested group, with the same declining trend for LOOH and MDA. These results do not conform with the hypothesis that mite attack induces oxidative stress and damage in Varroa-infested bees. The observed decline of lipid peroxidation will be addressed in further studies. This research was funded by The Ministry of Agriculture of the Czech Republic grant QK1910286.
ER -
PINĎÁKOVÁ, Eliška, Jiří DANIHLÍK, Silvie DOSTÁLKOVÁ, Marek PETŘIVALSKÝ, Pavel DOBEŠ, Jana HURYCHOVÁ a Pavel HYRŠL. Oxidative stress associated with varroa parasitism: evaluation of lipid peroxidation in infested bees. In \textit{EurBee 9 – 9th European Congress of Apidology}. 2022.
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