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@proceedings{2225525, author = {Šopíková, Tereza and Štveráková, Dana and Plachá, Kateřina and Benešík, Martin and Komárková, Marie and Botka, Tibor and Peichlová, Monika and Myslovičová, Nicol and Šiborová, Marta and Plevka, Pavel and Moša, Marek}, booktitle = {Viruses of Microbes 2022, Guimarães, Portugal, 2022}, keywords = {antibiotic crisis; phage therapy; phage collection; Staphylococcus aureus; Pseudomonas aeruginosa}, language = {eng}, title = {Phage collection against human and veterinary pathogens}, url = {https://skyros-congressos.pt/vom2022/}, year = {2022} }
TY - CONF ID - 2225525 AU - Šopíková, Tereza - Štveráková, Dana - Plachá, Kateřina - Benešík, Martin - Komárková, Marie - Botka, Tibor - Peichlová, Monika - Myslovičová, Nicol - Šiborová, Marta - Plevka, Pavel - Moša, Marek PY - 2022 TI - Phage collection against human and veterinary pathogens KW - antibiotic crisis KW - phage therapy KW - phage collection KW - Staphylococcus aureus KW - Pseudomonas aeruginosa UR - https://skyros-congressos.pt/vom2022/ N2 - When the world is facing an antibiotic crisis, it is necessary to investigate the development of alternative strategies. Phage therapy enables the specific treatment of bacterial infections. It is also possible to create highly efficient phage mutants to combat pathogenic bacterial strains that were initially resistant. The great diversity of bacterial strains, even at the species level, requires a variety of phages in collections that would be easily and quickly accessible in case of emergency. We aim to establish a collection of well-characterized phages applicable in human and veterinary medicine and to develop a quick phage isolation protocol, to be able to react to the actual needs of patients. Currently, our collection comprises phages against S. aureus, S. sciuri, P. aeruginosa, E. coli, A. salmonicida, A. hydrophila, K. pneumoniae, A. baumanii, and C. acnes. The phages were isolated using bacterial strains from the hospital environments, animal farms, and Masaryk University collection. The antimicrobial efficiency of phages was examined by spot test. The growth conditions of phages were optimized, and the stability of selected phages was tested. Selected phages were characterized in detail, and their genomes were sequenced. The chosen phages were purified, and their virions were depictured using transmission electron microscopy. ER -
ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA a Marek MOŠA. Phage collection against human and veterinary pathogens. In \textit{Viruses of Microbes 2022, Guimarães, Portugal, 2022}. 2022.
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