Phage collection against human and veterinary pathogens

a 2022

Phage collection against human and veterinary pathogens

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ et. al.

Základní údaje

Originální název

Phage collection against human and veterinary pathogens

Autoři

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA a Marek MOŠA

Vydání

Viruses of Microbes 2022, Guimarães, Portugal, 2022, 2022

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Portugalsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Viruses of Microbes 2022

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

antibiotic crisis; phage therapy; phage collection; Staphylococcus aureus; Pseudomonas aeruginosa

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 10. 2022 17:07, Mgr. Tibor Botka, Ph.D.

Anotace

V originále

When the world is facing an antibiotic crisis, it is necessary to investigate the development of alternative strategies. Phage therapy enables the specific treatment of bacterial infections. It is also possible to create highly efficient phage mutants to combat pathogenic bacterial strains that were initially resistant. The great diversity of bacterial strains, even at the species level, requires a variety of phages in collections that would be easily and quickly accessible in case of emergency. We aim to establish a collection of well-characterized phages applicable in human and veterinary medicine and to develop a quick phage isolation protocol, to be able to react to the actual needs of patients. Currently, our collection comprises phages against S. aureus, S. sciuri, P. aeruginosa, E. coli, A. salmonicida, A. hydrophila, K. pneumoniae, A. baumanii, and C. acnes. The phages were isolated using bacterial strains from the hospital environments, animal farms, and Masaryk University collection. The antimicrobial efficiency of phages was examined by spot test. The growth conditions of phages were optimized, and the stability of selected phages was tested. Selected phages were characterized in detail, and their genomes were sequenced. The chosen phages were purified, and their virions were depictured using transmission electron microscopy.

Citovat

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA a Marek MOŠA. Phage collection against human and veterinary pathogens. In Viruses of Microbes 2022, Guimarães, Portugal, 2022. 2022.

@proceedings{2225525,
   author = {Šopíková, Tereza and Štveráková, Dana and Plachá, Kateřina and Benešík, Martin and Komárková, Marie and Botka, Tibor and Peichlová, Monika and Myslovičová, Nicol and Šiborová, Marta and Plevka, Pavel and Moša, Marek},
   booktitle = {Viruses of Microbes 2022, Guimarães, Portugal, 2022},
   keywords = {antibiotic crisis; phage therapy; phage collection; Staphylococcus aureus; Pseudomonas aeruginosa},
   language = {eng},
   title = {Phage collection against human and veterinary pathogens},
   url = {https://skyros-congressos.pt/vom2022/},
   year = {2022}
}

TY  - CONF
ID  - 2225525
AU  - Šopíková, Tereza - Štveráková, Dana - Plachá, Kateřina - Benešík, Martin - Komárková, Marie - Botka, Tibor - Peichlová, Monika - Myslovičová, Nicol - Šiborová, Marta - Plevka, Pavel - Moša, Marek
PY  - 2022
TI  - Phage collection against human and veterinary pathogens
KW  - antibiotic crisis
KW  - phage therapy
KW  - phage collection
KW  - Staphylococcus aureus
KW  - Pseudomonas aeruginosa
UR  - https://skyros-congressos.pt/vom2022/
N2  - When the world is facing an antibiotic crisis, it is necessary to investigate the development of alternative strategies. Phage therapy enables the specific treatment of bacterial infections. It is also possible to create highly efficient phage mutants to combat pathogenic bacterial strains that were initially resistant. The great diversity of bacterial strains, even at the species level, requires a variety of phages in collections that would be easily and quickly accessible in case of emergency. We aim to establish a collection of well-characterized phages applicable in human and veterinary medicine and to develop a quick phage isolation protocol, to be able to react to the actual needs of patients. Currently, our collection comprises phages against S. aureus, S. sciuri, P. aeruginosa, E. coli, A. salmonicida, A. hydrophila, K. pneumoniae, A. baumanii, and C. acnes. The phages were isolated using bacterial strains from the hospital environments, animal farms, and Masaryk University collection. The antimicrobial efficiency of phages was examined by spot test. The growth conditions of phages were optimized, and the stability of selected phages was tested. Selected phages were characterized in detail, and their genomes were sequenced. The chosen phages were purified, and their virions were depictured using transmission electron microscopy.
ER  -

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA a Marek MOŠA. Phage collection against human and veterinary pathogens. In \textit{Viruses of Microbes 2022, Guimarães, Portugal, 2022}. 2022.

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