Phage collection against human and veterinary pathogens

ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA and Marek MOŠA. Phage collection against human and veterinary pathogens. In Viruses of Microbes 2022, Guimarães, Portugal, 2022. 2022.

Basic information

• Original name: Phage collection against human and veterinary pathogens
• Authors: ŠOPÍKOVÁ, Tereza, Dana ŠTVERÁKOVÁ, Kateřina PLACHÁ, Martin BENEŠÍK, Marie KOMÁRKOVÁ, Tibor BOTKA, Monika PEICHLOVÁ, Nicol MYSLOVIČOVÁ, Marta ŠIBOROVÁ, Pavel PLEVKA and Marek MOŠA.
• Edition: Viruses of Microbes 2022, Guimarães, Portugal, 2022, 2022.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Portugal
• Confidentiality degree: is not subject to a state or trade secret
• WWW: Viruses of Microbes 2022
• Organization unit: Faculty of Science
• Keywords in English: antibiotic crisis; phage therapy; phage collection; Staphylococcus aureus; Pseudomonas aeruginosa
• Tags: International impact, Reviewed

Abstract

When the world is facing an antibiotic crisis, it is necessary to investigate the development of alternative strategies. Phage therapy enables the specific treatment of bacterial infections. It is also possible to create highly efficient phage mutants to combat pathogenic bacterial strains that were initially resistant. The great diversity of bacterial strains, even at the species level, requires a variety of phages in collections that would be easily and quickly accessible in case of emergency. We aim to establish a collection of well-characterized phages applicable in human and veterinary medicine and to develop a quick phage isolation protocol, to be able to react to the actual needs of patients. Currently, our collection comprises phages against S. aureus, S. sciuri, P. aeruginosa, E. coli, A. salmonicida, A. hydrophila, K. pneumoniae, A. baumanii, and C. acnes. The phages were isolated using bacterial strains from the hospital environments, animal farms, and Masaryk University collection. The antimicrobial efficiency of phages was examined by spot test. The growth conditions of phages were optimized, and the stability of selected phages was tested. Selected phages were characterized in detail, and their genomes were sequenced. The chosen phages were purified, and their virions were depictured using transmission electron microscopy.