Identification of sex chromosomes using genomic and cytogenetic
methods in a range-expanding spider, Argiope bruennichi
(Araneae: Araneidae)

J 2022

Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae)

SHEFFER, Monica M ., Mathilde CORDELLIER, Martin FORMAN, Malte GREWOLDT, Katharina HOFFMANN et. al.

Základní údaje

Originální název

Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae)

Autoři

SHEFFER, Monica M . (garant), Mathilde CORDELLIER, Martin FORMAN, Malte GREWOLDT, Katharina HOFFMANN, Corinna JENSEN, Matěj KOTZ, Jiří KRÁL, Andreas W. KUSS, Eva LÍZNAROVÁ (203 Česká republika, domácí) a Gabriele UHL

Vydání

BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, ENGLAND, OXFORD UNIV PRESS, 2022, 0024-4066

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10602 Biology , Evolutionary biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.900

Kód RIV

RIV/00216224:14310/22:00126938

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1093/biolinnean/blac039

UT WoS

000800203100001

Klíčová slova anglicky

Araneae; Araneidae; Argiope bruennichi; genome size; karyotype; sex chromosome

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 1. 2023 13:40, Mgr. Marie Šípková, DiS.

Anotace

V originále

Differences between sexes in growth, ecology and behaviour strongly shape species biology. In some animal groups, such as spiders, it is difficult or impossible to identify the sex of juveniles based on external morphology. This information would be useful for field surveys, behavioural experiments and ecological studies, such as those on sex ratios and dispersal. In species with sex chromosomes, sex can be determined based on the specific sex chromosome complement. Additionally, information on the sequence of sex chromosomes provides the basis for studying sex chromosome evolution. We combined cytogenetic and genomic data to identify the sex chromosomes in the sexually dimorphic spider Argiope bruennichi and designed quantitative real-time polymerase chain reaction sex markers. We found that the genome size and GC content of this spider fall into the ranges reported for the majority of araneids. The male karyotype is formed by 24 acrocentric chromosomes with an X(1)X(2)0 sex chromosome system, with little similarity between X chromosomes, suggesting an origin of these chromosomes by X chromosome fission or early duplication of an X chromosome and subsequent independent differentiation of the copies. Our data suggest X chromosomes of similar sizes in A. bruennichi. They are smaller chromosomes of the complement. Our findings open the door to new directions in spider evolutionary and ecological research.

Citovat

SHEFFER, Monica M ., Mathilde CORDELLIER, Martin FORMAN, Malte GREWOLDT, Katharina HOFFMANN, Corinna JENSEN, Matěj KOTZ, Jiří KRÁL, Andreas W. KUSS, Eva LÍZNAROVÁ a Gabriele UHL. Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae). BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY. ENGLAND: OXFORD UNIV PRESS, 2022, roč. 136, č. 3, s. 405-416. ISSN 0024-4066. Dostupné z: https://dx.doi.org/10.1093/biolinnean/blac039.

@article{2225598,
   author = {Sheffer, Monica M . and Cordellier, Mathilde and Forman, Martin and Grewoldt, Malte and Hoffmann, Katharina and Jensen, Corinna and Kotz, Matěj and Král, Jiří and Kuss, Andreas W. and Líznarová, Eva and Uhl, Gabriele},
   article_location = {ENGLAND},
   article_number = {3},
   doi = {http://dx.doi.org/10.1093/biolinnean/blac039},
   keywords = {Araneae; Araneidae; Argiope bruennichi; genome size; karyotype; sex chromosome},
   language = {eng},
   issn = {0024-4066},
   journal = {BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY},
   title = {Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae)},
   url = {https://doi.org/10.1093/biolinnean/blac039},
   volume = {136},
   year = {2022}
}

TY  - JOUR
ID  - 2225598
AU  - Sheffer, Monica M . - Cordellier, Mathilde - Forman, Martin - Grewoldt, Malte - Hoffmann, Katharina - Jensen, Corinna - Kotz, Matěj - Král, Jiří - Kuss, Andreas W. - Líznarová, Eva - Uhl, Gabriele
PY  - 2022
TI  - Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae)
JF  - BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
VL  - 136
IS  - 3
SP  - 405-416
EP  - 405-416
PB  - OXFORD UNIV PRESS
SN  - 00244066
KW  - Araneae
KW  - Araneidae
KW  - Argiope bruennichi
KW  - genome size
KW  - karyotype
KW  - sex chromosome
UR  - https://doi.org/10.1093/biolinnean/blac039
N2  - Differences between sexes in growth, ecology and behaviour strongly shape species biology. In some animal groups, such as spiders, it is difficult or impossible to identify the sex of juveniles based on external morphology. This information would be useful for field surveys, behavioural experiments and ecological studies, such as those on sex ratios and dispersal. In species with sex chromosomes, sex can be determined based on the specific sex chromosome complement. Additionally, information on the sequence of sex chromosomes provides the basis for studying sex chromosome evolution. We combined cytogenetic and genomic data to identify the sex chromosomes in the sexually dimorphic spider Argiope bruennichi and designed quantitative real-time polymerase chain reaction sex markers. We found that the genome size and GC content of this spider fall into the ranges reported for the majority of araneids. The male karyotype is formed by 24 acrocentric chromosomes with an X(1)X(2)0 sex chromosome system, with little similarity between X chromosomes, suggesting an origin of these chromosomes by X chromosome fission or early duplication of an X chromosome and subsequent independent differentiation of the copies. Our data suggest X chromosomes of similar sizes in A. bruennichi. They are smaller chromosomes of the complement. Our findings open the door to new directions in spider evolutionary and ecological research.
ER  -

SHEFFER, Monica M ., Mathilde CORDELLIER, Martin FORMAN, Malte GREWOLDT, Katharina HOFFMANN, Corinna JENSEN, Matěj KOTZ, Jiří KRÁL, Andreas W. KUSS, Eva LÍZNAROVÁ a Gabriele UHL. Identification of sex chromosomes using genomic and cytogenetic methods in a range-expanding spider, Argiope bruennichi (Araneae: Araneidae). \textit{BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY}. ENGLAND: OXFORD UNIV PRESS, 2022, roč.~136, č.~3, s.~405-416. ISSN~0024-4066. Dostupné z: https://dx.doi.org/10.1093/biolinnean/blac039.

Podrobný výpis o publikaci