ROZSYPAL, Jan, Juraj ŠEVČÍK, Zdenka BARTOSOVA, Barbora PAPOUSKOVA, David JIROVSKY a Jan HRBÁČ. Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry. Microchemical Journal. Amsterdam: Elsevier, 2022, roč. 183, December, s. 1-8. ISSN 0026-265X. Dostupné z: https://dx.doi.org/10.1016/j.microc.2022.107955. |
Další formáty:
BibTeX
LaTeX
RIS
@article{2227879, author = {Rozsypal, Jan and Ševčík, Juraj and Bartosova, Zdenka and Papouskova, Barbora and Jirovsky, David and Hrbáč, Jan}, article_location = {Amsterdam}, article_number = {December}, doi = {http://dx.doi.org/10.1016/j.microc.2022.107955}, keywords = {Beer antioxidants; Antioxidant capacity; Polyphenols; HPLC; Electrochemical detection}, language = {eng}, issn = {0026-265X}, journal = {Microchemical Journal}, title = {Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry}, url = {https://www.sciencedirect.com/science/article/abs/pii/S0026265X22007834?via%3Dihub}, volume = {183}, year = {2022} }
TY - JOUR ID - 2227879 AU - Rozsypal, Jan - Ševčík, Juraj - Bartosova, Zdenka - Papouskova, Barbora - Jirovsky, David - Hrbáč, Jan PY - 2022 TI - Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry JF - Microchemical Journal VL - 183 IS - December SP - 1-8 EP - 1-8 PB - Elsevier SN - 0026265X KW - Beer antioxidants KW - Antioxidant capacity KW - Polyphenols KW - HPLC KW - Electrochemical detection UR - https://www.sciencedirect.com/science/article/abs/pii/S0026265X22007834?via%3Dihub N2 - Electrochemical techniques are rarely applied for the evaluation of total antioxidant capacity of beers due to severe electrode inactivation (fouling) by adsorbed oxidation products of the species present in the samples. In this work, we show that microdialysis online-coupled with amperometry is suitable for beer analysis, permitting also an assay automation. The working electrode of the amperometric detector employs a carbon fiber microelectrode that is regenerated between the analyses by short (1 sec) rapid (50 Hz) sinusoidal potential cycling between 0 and 2.9 V vs Ag/AgCl. This step, along with the sample cleanup by microdialysis effectively removes electrode fouling. The suitability of this approach for beer analysis was studied using eight different beers produced by international manufacturers. The results were compared with standard Kaneda diphenylpicrylhydrazil (DPPH) assay. Steady state currents, obtained at 600 mV vs Ag/AgCl correlate well with the results obtained with Kaneda method and thus can be used for quantification of beers' antioxidant capacities. HPLC-ED of microdialysates with and without DPPH treatment were employed to get an insight into DPPH reactivity with beer components. ER -
ROZSYPAL, Jan, Juraj ŠEVČÍK, Zdenka BARTOSOVA, Barbora PAPOUSKOVA, David JIROVSKY a Jan HRBÁČ. Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry. \textit{Microchemical Journal}. Amsterdam: Elsevier, 2022, roč.~183, December, s.~1-8. ISSN~0026-265X. Dostupné z: https://dx.doi.org/10.1016/j.microc.2022.107955.
|