| ROZSYPAL, Jan, Juraj ŠEVČÍK, Zdenka BARTOSOVA, Barbora PAPOUSKOVA, David JIROVSKY a Jan HRBÁČ. Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry. Microchemical Journal. Amsterdam: Elsevier, 2022, roč. 183, December, s. 1-8. ISSN 0026-265X. Dostupné z: https://dx.doi.org/10.1016/j.microc.2022.107955. |
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@article{2227879,
author = {Rozsypal, Jan and Ševčík, Juraj and Bartosova, Zdenka and Papouskova, Barbora and Jirovsky, David and Hrbáč, Jan},
article_location = {Amsterdam},
article_number = {December},
doi = {http://dx.doi.org/10.1016/j.microc.2022.107955},
keywords = {Beer antioxidants; Antioxidant capacity; Polyphenols; HPLC; Electrochemical detection},
language = {eng},
issn = {0026-265X},
journal = {Microchemical Journal},
title = {Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0026265X22007834?via%3Dihub},
volume = {183},
year = {2022}
}
TY - JOUR
ID - 2227879
AU - Rozsypal, Jan - Ševčík, Juraj - Bartosova, Zdenka - Papouskova, Barbora - Jirovsky, David - Hrbáč, Jan
PY - 2022
TI - Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry
JF - Microchemical Journal
VL - 183
IS - December
SP - 1-8
EP - 1-8
PB - Elsevier
SN - 0026265X
KW - Beer antioxidants
KW - Antioxidant capacity
KW - Polyphenols
KW - HPLC
KW - Electrochemical detection
UR - https://www.sciencedirect.com/science/article/abs/pii/S0026265X22007834?via%3Dihub
N2 - Electrochemical techniques are rarely applied for the evaluation of total antioxidant capacity of beers due to severe electrode inactivation (fouling) by adsorbed oxidation products of the species present in the samples. In this work, we show that microdialysis online-coupled with amperometry is suitable for beer analysis, permitting also an assay automation. The working electrode of the amperometric detector employs a carbon fiber microelectrode that is regenerated between the analyses by short (1 sec) rapid (50 Hz) sinusoidal potential cycling between 0 and 2.9 V vs Ag/AgCl. This step, along with the sample cleanup by microdialysis effectively removes electrode fouling. The suitability of this approach for beer analysis was studied using eight different beers produced by international manufacturers. The results were compared with standard Kaneda diphenylpicrylhydrazil (DPPH) assay. Steady state currents, obtained at 600 mV vs Ag/AgCl correlate well with the results obtained with Kaneda method and thus can be used for quantification of beers' antioxidant capacities. HPLC-ED of microdialysates with and without DPPH treatment were employed to get an insight into DPPH reactivity with beer components.
ER -
ROZSYPAL, Jan, Juraj ŠEVČÍK, Zdenka BARTOSOVA, Barbora PAPOUSKOVA, David JIROVSKY a Jan HRBÁČ. Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry. \textit{Microchemical Journal}. Amsterdam: Elsevier, 2022, roč.~183, December, s.~1-8. ISSN~0026-265X. Dostupné z: https://dx.doi.org/10.1016/j.microc.2022.107955.
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