| HRUŠKOVÁ, Helena, Ivona VORÁČOVÁ, Markéta LAŠTOVIČKOVÁ, Michael KILLINGER a František FORET. Preparative protein concentration from biofluids by epitachophoresis. Journal of Chromatography A. Elsevier B.V., 2022, roč. 1685, December, s. 1-6. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2022.463591. |
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@article{2229941,
author = {Hrušková, Helena and Voráčová, Ivona and Laštovičková, Markéta and Killinger, Michael and Foret, František},
article_number = {December},
doi = {http://dx.doi.org/10.1016/j.chroma.2022.463591},
keywords = {Proteins; Epitachophoresis; Concentration; Urine; Growth medium},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Preparative protein concentration from biofluids by epitachophoresis},
url = {https://www.sciencedirect.com/science/article/pii/S0021967322007828?via%3Dihub},
volume = {1685},
year = {2022}
}
TY - JOUR
ID - 2229941
AU - Hrušková, Helena - Voráčová, Ivona - Laštovičková, Markéta - Killinger, Michael - Foret, František
PY - 2022
TI - Preparative protein concentration from biofluids by epitachophoresis
JF - Journal of Chromatography A
VL - 1685
IS - December
SP - 1-6
EP - 1-6
PB - Elsevier B.V.
SN - 00219673
KW - Proteins
KW - Epitachophoresis
KW - Concentration
KW - Urine
KW - Growth medium
UR - https://www.sciencedirect.com/science/article/pii/S0021967322007828?via%3Dihub
N2 - With increasing demands on protein analyses in complex biological matrices, the insistence on developing new sample preparation techniques is rising. Recently, we introduced a new displacement electrophoresis technique (epitachophoresis) and instrumentation for preparative concentration and cleaning of DNA samples. This work describes the possibility of applying this device to protein samples. We have developed a method for the epitachophoretic concentration of proteins in a cationic mode and tested it by concentrating and collecting the protein zones from complex biological matrices (urine and growth medium). Under optimized conditions, we have obtained recoveries up to 99%. Furthermore, the applicability of the developed method was proven by concentrating and collecting the cytochrome c zone from a HeLa cell line growth medium, where the protein cytochrome c was released during cell apoptosis.
ER -
HRUŠKOVÁ, Helena, Ivona VORÁČOVÁ, Markéta LAŠTOVIČKOVÁ, Michael KILLINGER a František FORET. Preparative protein concentration from biofluids by epitachophoresis. \textit{Journal of Chromatography A}. Elsevier B.V., 2022, roč.~1685, December, s.~1-6. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2022.463591.
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