Preparative protein concentration from biofluids by
epitachophoresis

J 2022

Preparative protein concentration from biofluids by epitachophoresis

HRUŠKOVÁ, Helena, Ivona VORÁČOVÁ, Markéta LAŠTOVIČKOVÁ, Michael KILLINGER, František FORET et. al.

Základní údaje

Originální název

Preparative protein concentration from biofluids by epitachophoresis

Autoři

HRUŠKOVÁ, Helena (203 Česká republika, domácí), Ivona VORÁČOVÁ (garant), Markéta LAŠTOVIČKOVÁ, Michael KILLINGER (203 Česká republika, domácí) a František FORET

Vydání

Journal of Chromatography A, Elsevier B.V. 2022, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100

Kód RIV

RIV/00216224:14310/22:00127094

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chroma.2022.463591

UT WoS

000882393600006

Klíčová slova anglicky

Proteins; Epitachophoresis; Concentration; Urine; Growth medium

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 1. 2023 10:14, Mgr. Marie Šípková, DiS.

Anotace

V originále

With increasing demands on protein analyses in complex biological matrices, the insistence on developing new sample preparation techniques is rising. Recently, we introduced a new displacement electrophoresis technique (epitachophoresis) and instrumentation for preparative concentration and cleaning of DNA samples. This work describes the possibility of applying this device to protein samples. We have developed a method for the epitachophoretic concentration of proteins in a cationic mode and tested it by concentrating and collecting the protein zones from complex biological matrices (urine and growth medium). Under optimized conditions, we have obtained recoveries up to 99%. Furthermore, the applicability of the developed method was proven by concentrating and collecting the cytochrome c zone from a HeLa cell line growth medium, where the protein cytochrome c was released during cell apoptosis.

Citovat

HRUŠKOVÁ, Helena, Ivona VORÁČOVÁ, Markéta LAŠTOVIČKOVÁ, Michael KILLINGER a František FORET. Preparative protein concentration from biofluids by epitachophoresis. Journal of Chromatography A. Elsevier B.V., 2022, roč. 1685, December, s. 1-6. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2022.463591.

@article{2229941,
   author = {Hrušková, Helena and Voráčová, Ivona and Laštovičková, Markéta and Killinger, Michael and Foret, František},
   article_number = {December},
   doi = {http://dx.doi.org/10.1016/j.chroma.2022.463591},
   keywords = {Proteins; Epitachophoresis; Concentration; Urine; Growth medium},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Preparative protein concentration from biofluids by epitachophoresis},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967322007828?via%3Dihub},
   volume = {1685},
   year = {2022}
}

TY  - JOUR
ID  - 2229941
AU  - Hrušková, Helena - Voráčová, Ivona - Laštovičková, Markéta - Killinger, Michael - Foret, František
PY  - 2022
TI  - Preparative protein concentration from biofluids by epitachophoresis
JF  - Journal of Chromatography A
VL  - 1685
IS  - December
SP  - 1-6
EP  - 1-6
PB  - Elsevier B.V.
SN  - 00219673
KW  - Proteins
KW  - Epitachophoresis
KW  - Concentration
KW  - Urine
KW  - Growth medium
UR  - https://www.sciencedirect.com/science/article/pii/S0021967322007828?via%3Dihub
N2  - With increasing demands on protein analyses in complex biological matrices, the insistence on developing new sample preparation techniques is rising. Recently, we introduced a new displacement electrophoresis technique (epitachophoresis) and instrumentation for preparative concentration and cleaning of DNA samples. This work describes the possibility of applying this device to protein samples. We have developed a method for the epitachophoretic concentration of proteins in a cationic mode and tested it by concentrating and collecting the protein zones from complex biological matrices (urine and growth medium). Under optimized conditions, we have obtained recoveries up to 99%. Furthermore, the applicability of the developed method was proven by concentrating and collecting the cytochrome c zone from a HeLa cell line growth medium, where the protein cytochrome c was released during cell apoptosis.
ER  -

HRUŠKOVÁ, Helena, Ivona VORÁČOVÁ, Markéta LAŠTOVIČKOVÁ, Michael KILLINGER a František FORET. Preparative protein concentration from biofluids by epitachophoresis. \textit{Journal of Chromatography A}. Elsevier B.V., 2022, roč.~1685, December, s.~1-6. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2022.463591.

Podrobný výpis o publikaci