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@proceedings{2230725, author = {Šimečková, Hana and Obořilová, Radka and Pastucha, Matěj and Přibyl, Jan and Skládal, Petr and Pantůček, Roman and Mašlaňová, Ivana and Farka, Zdeněk}, booktitle = {Genetická konference GSGM 2022 a XXIX. Genetické dny}, keywords = {Bacteriophage; Atomic force microscopy; AFM; Surface plasmon resonance; SPR; Staphylococcus aureus; Lysis; Phage therapy}, language = {eng}, isbn = {978-80-7302-182-5}, title = {Phage-mediated bacterial lysis studied by AFM and SPR}, url = {https://gsgm2022.sci.muni.cz/}, year = {2022} }
TY - CONF ID - 2230725 AU - Šimečková, Hana - Obořilová, Radka - Pastucha, Matěj - Přibyl, Jan - Skládal, Petr - Pantůček, Roman - Mašlaňová, Ivana - Farka, Zdeněk PY - 2022 TI - Phage-mediated bacterial lysis studied by AFM and SPR SN - 9788073021825 KW - Bacteriophage KW - Atomic force microscopy KW - AFM KW - Surface plasmon resonance KW - SPR KW - Staphylococcus aureus KW - Lysis KW - Phage therapy UR - https://gsgm2022.sci.muni.cz/ N2 - Since the emergence and spread of multidrug-resistant bacterial strains exceed the development of new antimicrobial agents, an amount of research aimed at finding new therapeutic approaches is fostering. Suitable alternatives to combat bacterial infections include bacterial viruses (bacteriophages) or lytic enzymes (enzybiotics) as possible replacements or enhancers of conventional antibiotics. Phage therapy uses lytic phages to kill the bacterial host as a result of the virus life cycle, thus working with dynamic, living, and evolving entities. Detailed characterization of phage-mediated bacterial lysis is, therefore, essential. Biosensor-based techniques are sensitive and rapid analytical methods that provide detailed insight into lytic processes. As a surface imaging technique, atomic force microscopy (AFM) can be used to visualize cells and measure their mechanical properties. Biosensors based on surface plasmon resonance (SPR) record the interactions between biomacromolecules or small particles. Both of these approaches allow for monitoring immobilized cells under native conditions and in real-time. In our work, we investigate the effect of lytic antimicrobials against the pathogen Staphylococcus aureus. The study is focused on bacterial lysis mediated by the phage vB_SauP_P68 (P68) and the enzyme lysostaphin. AFM enabled a high-resolution investigation of topographical and biomechanical properties at the single cell level. The SPR experiments completed the work with the information on the kinetics of agent-bacterium interaction. The results are believed to help fight against bacterial infections and support the development of phage therapy. ER -
ŠIMEČKOVÁ, Hana, Radka OBOŘILOVÁ, Matěj PASTUCHA, Jan PŘIBYL, Petr SKLÁDAL, Roman PANTŮČEK, Ivana MAŠLAŇOVÁ a Zdeněk FARKA. Phage-mediated bacterial lysis studied by AFM and SPR. In \textit{Genetická konference GSGM 2022 a XXIX. Genetické dny}. 2022. ISBN~978-80-7302-182-5.
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